Identification of a Novel SNP Associated with Meat Quality in C/EBP慣 Gene of Korean Cattle

Shin, S. C.; Kang, M. J.; Chung, E. R.

doi:2007.20.4.466

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 2007;20(4): 466-470.
https://doi.org/10.5713/ajas.2007.466 Published online January 24, 2007.

Identification of a Novel SNP Associated with Meat Quality in C/EBP慣 Gene of Korean Cattle

S. C. Shin, M. J. Kang, E. R. Chung^*

Correspondence: E. R. Chung,

Abstract

CCAAT/enhancer binding protein ??(C/EBP?? plays an important role in lipid deposition and adipocyte differentiation. In order to find genetic markers to improve the meat quality of Korean cattle, the bovine C/EBP??gene was chosen as a candidate gene to investigate its association with carcass and meat quality traits in Korean cattle. A single nucleotide polymorphism (SNP) was identified at position 271 (A/C substitution) of coding region in the C/EBP??gene. A PCR-RFLP procedure with restriction enzyme SmaI was developed for determining the marker genotypes. The frequencies of alleles C and A and were 0.374 and 0.626, respectively. The genotype frequencies for CC, AC and AA were 12.9, 49.0 and 38.1%, respectively, in Korean cattle population. The frequencies of genotype were in agreement with Hardy-Weinberg equilibrium. Association analysis indicated that the gene-specific SNP marker of C/EBP??showed a significant association with marbling score (p<0.05). The animals with AA genotype had higher marbling score than those with the AC or CC genotype. Although further studies are needed to validate our results, the C/EBP??gene could be useful as a genetic marker for carcass and meat quality traits in Korean cattle.

Keywords: C/EBP??Gene; SNP Marker; Meat Quality; Korean Cattle