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Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.21.0497    [Accepted] Published online March 2, 2022.
Genetic diversity and population structure of Mongolian regional horses with 14 Microsatellite markers
Jihye Yun1  , Baatartsogt Oyungerel2  , Hong Sik Kong1,3,4,* 
1Department of Applied Biotechnology, The Graduate School of Hankyong National University, Anseong 17579, Korea
2School of Animal Science and Biotechnology, Mongolian University of Life Sciences, Zaisan 17024, Ulaanbaatar, Mongolia
3Genomic Informatics Center, Hankyong National University, Anseong 17579, Korea
4Gyeonggi Regional Research Center, Hankyong National University, Anseong 17579, Korea
Correspondence:  Hong Sik Kong, Tel: +82-31-670-5334, Fax: +82-504-842-4615, Email: kebinkhs@hknu.ac.kr
Received: 10 November 2021   • Revised: 10 January 2022   • Accepted: 16 February 2022
Abstract
Objective
This study aimed to identify the genetic diversity and population structure of Mongolian horse populations according to the province of residence (Khentii - KTP, Uvs - USP, Omnogovi and Dundgovi - GOP, Khovsgol - KGP) using 14 microsatellite (MS) markers.
Methods
A total of 269 whole blood samples were obtained from the four populations (KTP, USP, GOP, KGP) geographically distinct provinces. Multiplex PCR was conducted using 14 MS markers (AHT4, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG6, HTG7, and VHL20), as recommended by the International Society for Animal Genetics. Capillary electrophoresis was conducted using the amplified PCR products, alleles were determined. Alleles were used for statistical analysis of genetic variability, Nei’s DA genetic distance, principal coordinate analysis (PCoA), factorial corresponding analysis (FCA), and population structure.
Results
On average, the number of alleles, expected heterozygosity (HExp), observed heterozygosity (HObs), and polymorphic information content (PIC) among all populations were 11.43, 0.772, 0.757, and 0.737, respectively. In the PCoA and FCA, GOP and KGP were genetically distinct from other populations, and the KTP and USP showed a close relationship. The two cluster identified using Nei’s DA genetic distance analysis and population structure highlighted the presence of structurally clear genetic separation.

Conclusion

Overall, the results of this study suggest that genetic diversity between KTP and USP was low, and that between GOP and KGP was high. It is thought that these results will help in the effective preservation and improvement of Mongolian horses through genetic diversity analysis and phylogenetic relationships.
Keywords: Genetic Diversity; Horse; Microsatellites; Mongolia; Relationships; Structure


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