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Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.21.0461    [Accepted] Published online January 5, 2022.
Lactic acid bacteria strains selected from fermented total mixed rations improve ensiling and in vitro rumen fermentation characteristics of corn stover silage
Kailang Huang1  , Hongwei Chen1  , Yalu Liu1  , Qihua Hong2  , Bin Yang1,*  , Jiakun Wang1,* 
1Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China
2The Experimental Teaching Center, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China
Correspondence:  Bin Yang,Email: rayyangbin@163.com
Jiakun Wang, Tel: +86-571-8898-2389, Fax: +86-571-88982930, Email: jiakunwang@zju.edu.cn
Received: 9 October 2021   • Revised: 26 November 2021   • Accepted: 30 December 2021
This study identified the major lactic acid bacteria (LAB) strains from different fermented total mixed rations (FTMRs) via metataxonomic analysis and evaluate the ability of their standard strain as ensiling inoculants for corn stover silage.
The bacterial composition of eight FTMRs were analyzed by 16S rDNA sequencing. Corn stover was ensiled without LAB inoculation (control) or with 1×106 cfu/g LAB standard strain (Lactobacillus vaginalis, Lactobacillus reuteri, Lactobacillus helveticus, or Lactobacillus paralimentarius) selected from the FTMRs or 10 g/t commercial silage inoculant (CSI) around 25 °C for 56 days. For each inoculation, a portion of the silage was sampled to analyze ensiling characteristics at time intervals of 0, 1, 3, 7, 14, 28 and 56 days, Gas production (GP), microbial crude protein (MCP) and volatile fatty acids (VFAs) as the measurements of rumen fermentation characteristics were evaluated in vitro with the silages of 56 days after 72 h incubation.
Lactobacillus covered > 85% relative abundance of all FTMRs, in which L. pontis, L. vaginalis, L. reuteri, L. helveticus, and L. paralimentarius showed > 4% in specific FTMRs. CSI, L. helveticus, and L. paralimentarius accelerated the decline of silage pH. Silage inoculated with L. paralimentarius and CSI produced more lactic acid the early 14 days. Silage inoculated with L. paralimentarius produced less acetic acid and butyric acid. For the in vitro rumen fermentation, silage inoculated with CSI produced more potential GP, isobutyric acid, and isovaleric acid; silage inoculated with L. helveticus produced more potential GP and isovaleric acid, silage inoculated with L. paralimentarius or L. reuteri produced more potential GP only.


The standard strain L. paralimentarius (DSM 13238) is a promising ensiling inoculant for corn stover silage. The findings provide clues on strategies to select LAB to improve the quality of silage.
Keywords: Corn Stover Silage; Ensiling Characteristics; In vitro Rumen Fermentation; Lactobacillus; Metataxonomic Analysis

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