|
Stock No. | Botanical name | Scientific name | Family name | Partb |
---|---|---|---|---|
034–052 | Pomegranate | Punica granatum | Punicaceae | L+S+F |
015–042 | Brich | Betula schmidtii | Betulaceae | S |
019–076 | Ginkgo | Ginkgo biloba | Ginkgoaceae | L |
001–053 | Camellia | Camellia japonica | Theaceae | L |
026–020 | Tricuspid cudrania | Cudrania tricuspidata | Moraceae | L |
Target species | Primer sequence (5′→3′) | References |
---|---|---|
Total bacteria |
F: CGG CAA CGA GCG CAA CCC R: CCA TTG TAG CAC GTG TGT AGC C |
Denman and McSweeney (2006) |
Fibrobacter succinogenes |
F: GTT CGG AAT TAC TGG GCG TAA A R: CGC CTG CCC CTG AAC TAT C |
Denman and McSweeney (2006) |
Ruminococcus albus |
F: CCC TAA AAG CAG TCT TAG TTC G R: CCT CCT TGC GGT TAG AAC A |
Koike and Kobayashi (2001) |
Ruminocuccus flavefaciens |
F: CGA ACG GAG ATA ATT TGA GTT TAC TTA GG R: CGG TCT CTG TAT GTT ATG AGG TAT TAC C |
Denman and McSweeney (2006) |
Methanogenic archaea |
F: TTC GGT GGA TCD CAR AGR GC R: GBA RGT CGW AWC CGT AGA ATC C |
Denman et al. (2007) |
Ciliate protozoa |
F: GAG CTA ATA CAT GCT AAG GC R: CCC TCA CTA CAA TCG AGA TTT AAG G |
Skillman et al. (2006) |
Treatments | SEM | p-value | ||||||
---|---|---|---|---|---|---|---|---|
|
||||||||
Control | Pomegranate | Birch | Ginkgo | Camellia | Cudrania tricuspidata | |||
----------------------------------------------------------- pH ------------------------------------------------------ | ||||||||
6 h | 7.43a | 7.31a | 7.39a | 7.45a | 7.47a | 7.52a | 0.07 | 0.061 |
12 h | 7.07a | 7.06a | 7.04a | 7.08a | 7.15a | 7.15a | 0.04 | 0.143 |
24 h | 6.67 a | 6.71 a | 6.71 a | 6.69 a | 6.69 a | 6.71 a | 0.04 | 0.637 |
48 h | 6.45 a | 6.46 a | 6.47 a | 6.47 a | 6.52 a | 6.49 a | 0.05 | 0.523 |
72 h | 6.48b | 6.47b | 6.68a | 6.65a | 6.74a | 6.66a | 0.07 | 0.002 |
---------------------------------------------------- tVFA (mM) --------------------------------------------------- | ||||||||
6 h | 30.96 a | 37.02 a | 30.66 a | 32.46 a | 30.90 a | 28.74 a | 4.73 | 0.424 |
12 h | 37.99b | 39.66ab | 39.09b | 40.05ab | 38.27b | 42.28a | 1.45 | 0.037 |
24 h | 64.08a | 60.46a | 59.93a | 61.72a | 62.49a | 62.16a | 1.87 | 0.168 |
48 h | 78.21a | 73.37abc | 74.79ab | 69.03bc | 67.43c | 70.87bc | 3.77 | 0.041 |
72 h | 75.80a | 76.05a | 64.92ab | 66.36ab | 57.51b | 70.21a | 6.49 | 0.033 |
--------------------------------------------------- DM degradability (%) ---------------------------------------- | ||||||||
6 h | 22.61a | 22.17a | 21.91a | 22.10a | 21.90a | 20.58b | 0.57 | 0.018 |
12 h | 25.94 a | 25.59 a | 24.18 a | 24.65 a | 23.90 a | 27.67 a | 2.83 | 0.617 |
24 h | 31.19a | 30.67a | 30.90a | 29.85a | 29.72a | 28.50a | 1.10 | 0.096 |
48 h | 40.69 a | 41.88 a | 41.78 a | 39.65 a | 41.03 a | 40.80 a | 1.29 | 0.362 |
72 h | 41.59b | 46.17a | 32.82c | 41.78b | 34.72c | 43.04b | 1.57 | 0.000 |
Treatments | SEM | p-value | ||||||
---|---|---|---|---|---|---|---|---|
|
||||||||
Control | Pomegranate | Birch | Ginkgo | Camellia | Cudrania tricuspidata | |||
---------------------------------------------- Total gas (mL/g DM) --------------------------------------- | ||||||||
6 h | 142.9ab | 143.0ab | 143.7a | 144.0a | 144.0a | 142.0b | 0.46 | 0.061 |
12 h | 170.3b | 179.1a | 181.1a | 179.2a | 177.1a | 175.5ab | 2.02 | 0.033 |
24 h | 218.9 a | 221.0 a | 220.3 a | 220.4 a | 220.4 a | 219.5 a | 2.33 | 0.989 |
48 h | 258.5 b | 272.2 a | 237.8 c | 277.9 a | 245.1 c | 275.5 a | 3.97 | <.0001 |
72 h | 274.3b | 294.9a | 237.8c | 278.1b | 245.1c | 276.8b | 3.18 | 0.006 |
---------------------------------------------------- CH4 (mL/g DM) ------------------------------------------ | ||||||||
6 h | 1.70 a | 1.63 a | 2.09 a | 1.57 a | 1.60 a | 1.47 a | 0.26 | 0.610 |
12 h | 6.24a | 6.25a | 5.46a | 5.59a | 5.63a | 5.47a | 0.45 | 0.632 |
24 h | 15.87a | 8.31b | 9.59b | 8.46b | 8.27b | 8.12b | 1.35 | 0.010 |
48 h | 29.90ab | 22.81ab | 16.99b | 32.91a | 22.61ab | 35.70a | 5.20 | 0.170 |
72 h | 39.64ab | 44.96a | 30.16c | 40.27ab | 35.82bc | 37.69abc | 2.36 | 0.017 |
---------------------------------------------------- CO2 (mL/g DM) ----------------------------------------- | ||||||||
6 h | 19.73a | 16.55ab | 18.90ab | 15.42ab | 16.04ab | 14.81b | 1.72 | 0.384 |
12 h | 44.08a | 52.74a | 48.39a | 49.05a | 49.75a | 48.43a | 3.37 | 0.643 |
24 h | 77.47a | 59.12a | 74.65a | 64.28a | 67.89a | 62.48a | 9.23 | 0.705 |
48 h | 102.1ab | 109.0ab | 74.99d | 93.65bc | 78.14cd | 116.6a | 5.41 | 0.001 |
72 h | 104.4a | 115.6a | 103.9a | 110.5a | 107.81a | 100.5a | 6.30 | 0.613 |