Go to Top Go to Bottom
Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.22.0432    [Accepted] Published online May 2, 2023.
Genetic diversity and selection of Tibetan sheep breeds revealed by whole-genome resequencing
Dehong Tian1,2,3  , Buying Han1,2,3  , Xue Li1,2,3  , Dehui Liu1,2,3  , Baicheng Zhou4  , Chunchuan Zhao5  , Nan Zhang5  , Lei Wang6  , Quanbang Pei6  , Kai Zhao1,3,* 
1Key Laboratory of Adaptation and Evolution of Plateau Biota, Qinghai Provincial Key Laboratory of Animal Ecological Genomics, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, Qinghai, China
2University of Chinese Academy of Sciences, Beijing 100049, China
3Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
4General Station of Animal Husbandry of Qinghai Province, Xining 810001, Qinghai, China
5Qinghai Conservation and Utilization Center of Livestock and Poultry Genetic Resources, Xining 810000, Qinghai, China
6Qinghai Sheep Breeding and Promotion Service Center, Gangcha 812300, Qinghai, China
Correspondence:  Kai Zhao,Email: peiquanbang@163.com
Received: 12 November 2022   • Revised: 29 January 2023   • Accepted: 5 March 2023
To elucidate the underlying gene regions responsible for productive, phenotypic or adaptive traits in different ecological types of Tibetan sheep and the discovery of important genes encoding valuable traits.
We used whole-genome resequencing to explore the genetic relationships, phylogenetic tree, and population genetic structure analysis. In addition, we identified 28 representative Tibetan sheep single-nucleotide polymorphisms (SNPs) and genomic selective sweep regions with different traits in Tibetan sheep by fixation index (Fst) and the nucleotide diversity (θπ) ratio.
The genetic relationships analysis showed that each breed partitioned into its own clades and had close genetic relationships. We also identified many potential breed-specific selective sweep regions, including genes associated with hypoxic adaptability (MTOR, TRHDE, PDK1, PTPN9 TMTC2, SOX9, EPAS1, PDGFD, SOCS3, TGFBR3), coat color (MITF, MC1R, ERCC2, TCF25, ITCH, TYR, RALY, KIT), wool traits (COL4A2, ERC2, NOTCH2, ROCK1, FGF5, SOX9), and horn phenotypes (RXFP2). In particular, a horn-related gene, RXFP2, showed the four most significantly associated SNP loci (g. 29481646 A>G, g. 29469024 T>C, g. 29462010 C>T, g. 29461968 C>T) and haplotypes.
This finding demonstrates the potential for genetic markers in future molecular breeding programs to improve selection for horn phenotypes. The results will facilitate the understanding of the genetic basis of production and adaptive unique traits in Chinese indigenous Tibetan sheep taxa and offer a reference for the molecular breeding of Tibetan sheep.
Keywords: Haplotypes; Hypoxic Adaptability; Indigenous Breeds; Selective Sweep; Production

Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul 08776, Korea   
TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : animbiosci@gmail.com               

Copyright © 2023 by Asian-Australasian Association of Animal Production Societies.

Developed in M2PI

Close layer
prev next