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Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.22.0227    [Accepted] Published online November 14, 2022.
Novel polymorphisms of dopa decarboxylase (DDC) gene and their association with lamb quality traits in Indonesian sheep
Ratna Sholatia Harahap1  , Ronny Rachman Noor2  , Yuni Cahya Endrawati2  , Huda Shalahudin Darusman3  , Asep Gunawan2,* 
1Graduate School of Animal Production and Technology, Faculty of Animal Science, IPB University, Jl. Agatis, Kampus IPB Darmaga Bogor 16680, Indonesia
2Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Jl. Agatis, Kampus IPB Darmaga Bogor 16680, Indonesia
3Department of Anatomy Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University, Jl. Agatis, Kampus IPB Darmaga Bogor 16680, Indonesia
Correspondence:  Asep Gunawan, Tel: +62-251-8622841, Fax: +62-251-8622841, Email: agunawan@apps.ipb.ac.id
Received: 9 June 2022   • Revised: 6 August 2022   • Accepted: 2 November 2022
Abstract
Objective
This study aimed to investigate the polymorphisms of the dopa decarboxylase (DDC) gene and association analysis with lamb quality and expression quantification of the DDC gene in phenotypically divergent Indonesian sheep.
Methods
The totals of 189 rams with an average body weight of 24.12 kg at 10-12 months were used to identify DDC gene polymorphism using PCR-RFLP. Among 189 rams, several rams representing various sheep genotypes were used for an association study between genotypes and phenotypic traits with proc GLM analysis. In addition, the gene expression analysis of the DDC mRNA in the phenotypically divergent sheep population was analyzed using qRT-PCR.
Results
The DDC gene (g. 5377439 G>A) showed polymorphisms that indicated three genotypes: AA, AG, and GG. The DDC gene polymorphism was significantly associated (P≤0.05) with carcass characteristics including carcass percentage, carcass length, hot and cold carcass; physical properties of lamb quality including pH value; retail cut carcass; fatty acid composition such as fat content, pentadecanoic acid (C15:0), tricosylic acid (C23:0), lignoceric acid (C24:0), oleic acid (C18:1n9c), elaidic acid (C18:1n9t), nervonic acid (C24:1), linoleic acid (C18:2n6c), arachidonic acid (C20:4n6), cervonic acid (C22:6n3); and mineral content including potassium (K). The GG genotype of the DDC gene had the best association with lamb quality traits. The DDC gene expression analysis mRNA showed no significant difference (P≥0.05) between lamb quality traits.
Conclusion
The DDC gene could be used as a potential candidate gene to improve lamb quality.
Keywords: Carcass Characteristics; DDC Gene; Fatty Acid Composition; Lamb Quality; Retail Meat Cut


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