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Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.22.0165    [Accepted] Published online November 13, 2022.
Comprehensive analysis of miRNAs, lncRNAs and mRNAs profiles in backfat tissue between Daweizi and Yorkshire pigs
Chen Chen1,*  , Yitong Chang2  , Yuan Deng1  , Qingming Cui1  , Yingying Liu1  , Huali Li1  , Huibo Ren1  , Ji Zhu1  , Qi Liu3  , Yingling Peng1,2,* 
1Hunan Institute of Animal & Veterinary Science, Changsha, 410131, China
2College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, China
3Hunan Tianfu Ecological Agricultural Limited Company, Changsha, 410144, China
Correspondence:  Chen Chen,Email: 2004chch@163.com
Yingling Peng, Tel: +86-731-84615328, Fax: +86-731-84611342, Email: Ylpeng_1965@163.com
Received: 24 April 2022   • Revised: 2 August 2022   • Accepted: 11 September 2022
Abstract
Objective
Daweizi (DWZ) is a famous indigenous pig breed in China and characterized by tender meat and high fat percentage. However, the expression profiles and functions of transcripts in DWZ pigs is still in infancy. The object of this study was to depict the transcript profiles in DWZ pigs and screen the potential pathway influence adipogenesis and fat deposition,
Methods
Histological analysis of backfat tissue was firstly performed between DWZ and lean-type Yorkshire pigs, and then RNA sequencing technology was utilized to explore miRNAs, lncRNAs and mRNAs profiles in backfat tissue. 18 DE transcripts were randomly selected for quantitative real-time polymerase chain reaction (QPCR) to validate the reliability of the sequencing results. Finally, GO and KEGG enrichment analysis were conducted to investigate the potential pathways influence adipocyte differentiation, adipogenesis and lipid metabolism, and a schematic model was further proposed.
Results
A total of 1625 differentially expressed transcripts were identified in DWZ pigs, including 27 upregulated and 45 downregulated miRNAs, 64 upregulated and 119 downregulated lncRNA, 814 upregulated and 556 downregulated mRNAs. QPCR analysis exhibited strong consistency with the sequencing data. GO and KEGG analysis elucidated that the differentially expressed transcripts were mainly associated with cell growth and death, signal transduction, PPAR, AMPK, PI3K-Akt, adipocytokine and foxo signaling pathways, all of which are strongly involved in cell development, lipid metabolism and adipogenesis. Further analysis indicated that the BGIR9823_87926/miR-194a-5p/AQP7 network may effective in the process of adipocyte differentiation or adipogenesis.
Conclusion
Our study provides comprehensive insights into the regulatory network of backfat deposition and lipid metabolism in pigs from the point of view of miRNAs, lncRNAs and mRNAs.
Keywords: Backfat; DWZ Pigs; Yorkshire Pigs; RNA Sequencing; Transcript Profile; Pathway


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