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https://doi.org/10.5713/ab.21.0174    [Accepted] Published online August 25, 2021.
GOT1 regulates adipocyte differentiation by altering NADPH content
Yang Yang1  , Zhimin Cheng1,2  , Wanfeng Zhang1  , Wei Hei1  , Chang Lu1  , Chunbo Cai1  , Yan Zhao1  , Pengfei Gao1  , Xiaohong Guo1  , Guoqing Cao1,*  , Bugao Li1,* 
1College of Animal Science, Shanxi Agricultural University, Taigu 030801, China
2Shanxi Academy of Advanced Research and Innovation, Taiyuan 030032, China
Correspondence:  Guoqing Cao,Email: anniecao710502@aliyun.com
Bugao Li, Tel: +86-138-3540-1655, Fax: +86-0354-6289295, Email: jinrenn@163.com
Received: 14 April 2021   • Revised: 24 May 2021   • Accepted: 27 July 2021
Abstract
Objective
This study was performed to examine whether the porcine GOT1 gene had important functions in regulating adipocyte differentiation.
Methods
Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipocytic 3T3-L1 cells. Lipid droplet levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of MDH1 and ME1 and the cellular NADPH content.
Results
GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (P<0.01), whereas GOT1 overexpression significantly increased lipid accumulation (P<0.01). At the same time, GOT1 knockout significantly decreased the NADPH content and the expression of MDH1 and ME1 in the 3T3-L1 cells. Overexpression of GOT1 significantly increased the NADPH content and the expression of MDH1 and ME1, suggesting that GOT1 regulated adipocyte differentiation by altering the NADPH content.

Conclusion

The results preliminarily revealed the effector mechanisms of GOT1 in regulating adipose differentiation. Thus, a theoretical basis is provided for improving the quality of pork and studies on diseases associated with lipid metabolism.
Keywords: Adipogenesis; Intramuscular Fat; Lipid Metabolism; GOT1, NADPH
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