|
Two Maternal Lineages Revealed by Mitochondrial DNA D-loop Sequences in Chinese Native Water Buffaloes (Bubalus bubalis) |
|
Chu-Zhao Lei, Wei Zhang, Hong Chen, Fan Lu, Qing-Lan Ge, Ruo-Yu Liu, Rui-Hua Dang, Yun-Yi Yao, Li-Bo Yao, Zi-Fan Lu*, Zhong-liang Zhao |
|
|
Correspondence:
Zi-Fan Lu,
|
|
|
|
|
|
| Abstract |
|
Little is known about the origin and genetic diversity of swamp buffaloes in China. To obtain more knowledge on genetics of the water buffalo in China, the complete mitochondrial D-loop sequences of 30 samples from 6 native types were investigated. The results revealed 12 mitochondrial haplotypes with 50 polymorphic sites. Among these polymorphic sites, there were 49 transitions and 1 transversion. The average nucleotide diversity and haplotype diversity estimated from mtDNA D-loop region in 6 Chinese water buffalo types were 0.00684 and 0.798, respectively, showing rather abundant mitochondrial genetic diversity. The Neighbor-Joining (NJ) tree of mtDNA of Chinese water buffaloes was constructed according to the 12 haplotypes. The NJ tree indicated two lineages being designated lineage A and lineage B, in which lineage A was predominant, and lineage B was at low frequency. The new lineage B was first discovered and defined in 6 Chinese water buffalo types. These results showed that two different maternal lineages were involved in the origin of domestic swamp buffaloes in China and the lineage B was probably an introgression from Southeast Asian buffaloes. |
| Keywords:
Chinese Water Buffalo; Mitochondrial DNA D-loop; Diversity; Haplotype; Origin |
|
PDF Links
Full text via DOI
Download Citation
