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Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.23.0543    [Accepted] Published online April 26, 2024.
Effects of prilled fat supplementation in diets with varying protein levels on production performance of early lactating Nili Ravi Buffaloes
Saba Anwar1  , Anjum Khalique2  , Hifzulrahman 3  , Muhammad Naeem Tahir4  , Burhan EAzam5, Muhammad Asim Tausif5  , Sundas Qamar1  , Hina Tahir1  , Mutraza Ali Tipu6  , Muhammad Naveed ul Haque2,* 
1Buffalo Research Institute, Pattoki District Kasur, 53000, Pakistan
2Department of Animal Nutrition, University of Veterinary & Animal Sciences, Outfall road, Lahore 54000, Pakistan
3Department of Livestock Production, University of Veterinary & Animal Sciences, Outfall road, Lahore 54000, Pakistan
4Department of Livestock Management, Faculty of Veterinary and Animal Sciences, The Islamia University Bahawalpur 63100, Pakistan
5Livestock Experiment Station, Bhunikey, Pattoki, District Kasur, 53000, Pakistan
6Department of Livestock and Dairy Development, 16 Cooper Road, Lahore 54000, Pakistan
Correspondence:  Muhammad Naveed ul Haque, Tel: +92-333-4783691, Email: muhammad.naveed@uvas.edu.pk
Received: 26 December 2023   • Revised: 13 February 2024   • Accepted: 1 April 2024
The objective of the current study was to find out the independent and interactive effects of prilled fat supplementation with protein on the production performance of early lactating Nili Ravi buffaloes.
Sixteen early lactating buffaloes (36.75 ± 5.79 d in milk; mean ± SE) received 4 treatments in 4 × 4 Latin-square design according to 2 × 2 factorial arrangements. The dietary treatments were:1) LPLF = low protein low fat, 2) LPHF = low protein high fat, 3) HPLF = high protein low fat, and 4) HPHF = high protein high fat. The dietary treatments contained 2 protein (8.7 and 11.7% CP, crude protein) and fat levels (2.6 and 4.6% EE, ether extract) on a dry matter basis.
The yields of milk and fat increased with increasing protein and fat independently (p≤0.05). Energy-, protein-, and fat-corrected milk yields also increased with increasing protein and fat independently (p≤0.05). Increasing dietary protein increased the protein yield by 3.75% and lactose yield by 3.15% and increasing dietary fat supplies increased the fat contents by 3.93% (p≤0.05). Milk yield and fat-corrected milk to dry matter intake ratios were increased at high protein and high fat levels (p≤0.05). Milk nitrogen efficiency was unaffected by dietary fat (p>0.10), whereas it decreased with increasing protein supplies (p≤0.05). Plasma urea nitrogen and cholesterol were increased by increasing protein and fat levels, respectively (p≤0.05). The values of predicted methane production reduced with increasing dietary protein and fat.
It is concluded that prilled fat and protein supplies increased milk and fat yield along with increased ratios of milk yield and fat-corrected milk yields to dry matter intake. However, no interaction was observed between prilled fat and protein supplementation for production parameters, body weight, BCS and blood metabolites. Predicted methane production decreased with increasing protein and fat levels.
Keywords: Buffalo; Methane; Milk Production; Protein Supplies; Rumen Inert Fat

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