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The cooperative regulatory effect of the miR-130 family on milk fat metabolism in dairy cows |
Xiaofen Li1,* 
, Yanni Wu2
, Xiaozhi Yang1
, Rui Gao2
, Qinyue Lu5
, Xiaoyang Lv3,4
, Zhi Chen2
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1School of Pet Technology, Jiangsu Agri-animal Husbandry Vocational College, Taizhou, 225300, China
2College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
3Joint International Research Laboratory of Agriculture & Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, China
4International Joint Research Laboratory in Universities of Jiangsu Province of China for Domestic Animal Germplasm Resources and Genetic Improvement, Yangzhou 225009, China
5Laboratory of Animal Developmental Biology, Department of Animal Science, Chungbuk National University, Cheongju 28644, Korea |
Correspondence:
Xiaofen Li, Tel: +86-18252713205, Fax: +86-0514-84212634, Email: lxf@jsahvc.edu.cn
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Received: 15 November 2023 • Revised: 2 January 2024 • Accepted: 2 March 2024 |
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| Abstract |
Objective
There is a strong relationship between the content of beneficial fatty acids in milk and milk fat metabolic activity in the mammary gland. To improve milk quality, it is therefore necessary to study fatty acid metabolism in bovine mammary gland tissue. In adipose tissue, peroxisome proliferator-activated receptor gamma (PPARG), the core transcription factor, regulates the fatty acid metabolism gene network and determines fatty acid deposition. However, its regulatory effects on mammary gland fatty acid metabolism during lactation have rarely been reported.
Methods
Transcriptome sequencing was performed during the prelactation period and the peak lactation period to examine mRNA expression. The significant upregulation of PPARG drew our attention and led us to conduct further research.
Results
According to bioinformatics prediction, dual-luciferase reporter system detection, qRT‒PCR and Western blotting, miR-130a and miR-130b could directly target PPARG and inhibit its expression. Furthermore, triglyceride and Oil Red O staining proved that miR-130a and miR-130b inhibited milk fat metabolism in BMECs, while PPARG promoted this metabolism. In addition, we also found that the coexpression of miR-130a and miR-130b significantly enhanced their ability to regulate milk fat metabolism.
Conclusion
In conclusion, our findings indicated that miR-130a and miR-130b could target and repress PPARG and that they also have a functional superposition effect. miR-130a and miR-130b seem to synergistically regulate lipid catabolism via the control of PPARG in BMECs. In the long-term, these findings might be helpful in developing practical means to improve high-quality milk.
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| Keywords:
Dairy Cows, Milk Fat Metabolism; miR-130a; miR-130b; PPARG |
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