Improvement of rooster semen freezability and fertility rate after sericin supplementation in freezing semen extender

Ratchamak, Ruthaiporn; Authaida, Supakorn; Boonkum, Wuttigrai; Chankitisakul, Vibuntita

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Anim Biosci > Accepted Articles

Article

https://doi.org/10.5713/ab.23.0016 [Accepted] Published online May 2, 2023.

Improvement of rooster semen freezability and fertility rate after sericin supplementation in freezing semen extender

Ruthaiporn Ratchamak^1,2

, Supakorn Authaida^1,2

, Wuttigrai Boonkum^1,2

, Vibuntita Chankitisakul^1,2,*

¹Department of Animal Science, Faculty of Agricultural, Khon Kaen University, Khon Kaen 40002, Thailand
²The Research and Development Network Center of Animal Breeding and Omics, Khon Kaen University, Khon Kaen 40002, Thailand

Correspondence: Vibuntita Chankitisakul, Tel: +66-43202362, Fax: +66-43202362, Email: vibuch@kku.ac.th

Received: 20 January 2023 • Revised: 3 March 2023 • Accepted: 21 March 2023

Abstract

Objective
The effects of semen cryopreservation result in decreased sperm parameters and fertilization ability. Sericin exhibits antioxidant activity by reducing lipid peroxidation resulting from free radicals, which can potentially improve cryopreservation outcomes. The present study aimed to examine the efficacy of various sericin concentrations supplemented with a rooster semen-freezing extender on post-thaw semen quality and fertilizing ability of sperm after cryopreservation.
Methods
Semen samples were collected from 40 roosters (5 reps), then were pooled and divided into four groups by the levels of sericin supplementation (0, 0.25, 0.50, and 0.75%) in a freezing extender. Semen suspensions were loaded in medium straw (0.5 mL) and cryopreserved with the traditional liquid nitrogen vapor method. Post-thawed semen was evaluated for sperm motility, sperm viability, and lipid peroxidation. Also, the fertility test was determined.
Results
The results showed that supplementation of the freezing extender with 0.50-0.75% sericin resulted in greater total motility and progressive motility and lower MDA levels than the other groups after cryopreservation (p<0.05). However, the viability of 0.75% decreased compared with the value of 0.50% (p<0.05). Moreover, the fertility and hatchability of total eggs were significantly higher in the 0.50% sericin group than in the other groups (p<0.05).
Conclusion
In conclusion, 0.50% sericin is recommended as an alternative component of the freezing extender to improve cryopreserved rooster semen.

Keywords: Cryopreservation; Frozen-thaw Semen; Lipid Peroxidation; Thai Native Chicken