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Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.22.0308    [Accepted] Published online January 11, 2023.
Effect of ACADL on the differentiation of goat subcutaneous adipocyte
A Li1,2,3  , YY Li1,2,3  , QB Wuqie1,2,3  , X Li1,2,3  , H Zhang1,2,3  , Y Wang1,2,3  , YL Wang1,2,3  , JJ Zhu1,2,3  , YQ Lin1,2,3,* 
11 Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, China
2Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation of Sichuan Province, Southwest Minzu University, Chengdu, China
3College of Animal & Veterinary Science, Southwest Minzu University, Chengdu, China
Correspondence:  YQ Lin,Email: linyq1999@163.com
Received: 9 August 2022   • Revised: 19 November 2022   • Accepted: 24 December 2022
Abstract
Objective
The aim of this study was to clone the mRNA sequence of the ACADL gene of goats and explore the effect of ACADL on the differentiation of subcutaneous fat cells on this basis.
Methods
We obtained the ACADL gene of goats by cloning and used -qPCR to detect the ACADL expression patterns of different goat tissues and subcutaneous fat cells at different lipid induction stages. In addition, we transfect intramuscular and subcutaneous adipocytes separately by constructing overexpressed ACADL vectors and synthesizing Si-ACADL; Finally, we observed the changes in oil red stained cell levels under the microscope, and qPCR detected changes in mRNA levels.
Results
The results showed goat ACADL gene expressed in sebum fat. During adipocyte differentiation, ACADL gradually increased from 0 to 24 h of culture, and decreased. Overexpression of ACADL promoted differentiation of subcutaneous adipocytes in goat and inhibited their differentiation after interference.
Conclusion
So, we infer ACADL may have an important role in positive regulating the differentiation process in goat subcutaneous adipocytes. This study will provide basic data for further study of the role of ACADL in goat subcutaneous adipocyte differentiation and lays the foundation for final elucidating of its molecular mechanisms in regulating subcutaneous fat deposition in goats.
Keywords: ACADL; Adipocytes; Differentiation; Goat; Subcutaneous


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