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Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.22.0274    [Accepted] Published online January 8, 2023.
Effect of dietary supplementation of Bacillus subtilis TLRI 211-1 on laying performance, egg quality and blood characteristics of Leghorn layers
Ming Yang Tsai1,2  , Bor Ling Shih3  , Ren Bao Liaw4  , Tsung Yu Lee3  , Wen Tsen Chen3  , Hsi Wen Hung3  , Kuo Hsiang Hung2,*  , Yih Fwu Lin3,* 
1Animal Industry Division, Livestock Research Institute (LRI), Council of Agriculture (COA), No. 112, Farm Rd, Hsinhua, Tainan, 712009, Taiwan
2Graduate Institute of Bioresources, National Pingtung University of Science and Technology. No. 1, Shuefu Road, Neipu, Pingtung 91201, Taiwan
3Nutrition Division, Livestock Research Institute (LRI), Council of Agriculture (COA), Hsinhua, Tainan, 712009, Taiwan
4Physiology Division, Livestock Research Institute (LRI), Council of Agriculture (COA), Hsinhua, Tainan, 712009, Taiwan
Correspondence:  Kuo Hsiang Hung,Email: khhung424@mail.npust.edu.tw
Yih Fwu Lin,Email: a0927430021@gmail.com
Received: 12 July 2022   • Revised: 10 October 2022   • Accepted: 15 November 2022
Abstract
Objective
TLRI 211-1 is a novel Bacillus subtilis strain. This experiment was to investigate dietary supplementation of TLRI 211-1 on laying performance, egg quality and blood characteristics of layers.
Methods
One hundred and twenty 65-wk-old Leghorn layers were divided into four treatment groups for 8 weeks experiment. Each treatment had three replicates. The basal diet was formulated as control group with CP 17% and ME 2,850 kcal/kg and supplemented with TLRI 211-1 0.1%, 0.3%, and commercial Bacillus amyloliquefaciens 0.1% as treatment 2, 3 and 4 groups, respectively. Both TLRI 211-1 and commercial Bacillus amyloliquefaciens were adjusted to contain 1 x 109 CFU/mL (g), hence the 0.1% supplemental level was 1 x 109 CFU/kg.
Results
The results showed that TLRI 211-1 0.3% and commercial B. amyloliquefaciens groups had higher weight gain than the other groups; TLRI 211-1 0.1% group had better feed to eggs conversion ratio than the control and commercial B. amyloliquefaciens groups (P < 0.05). Bacillus subtilis supplementation increased yolk weight (P < 0.05). In egg quality during storage, TLRI 211-1 0.1% had higher breaking strength than the control group at the second week of storage (P < 0.05). At the third week of storage, TLRI 211-1 0.3% had higher Haugh unit (P < 0.05). Hens fed diets supplemented with TLRI 211-1 0.3% significantly decreased blood triglyceride levels and increased blood calcium levels (P < 0.05). TLRI 211-1 0.3% group had lower H2S (P < 0.05) and hence had less unpleasant odor in excreta of hens.
Conclusion
In conclusion, supplementation with 0.1% TLRI 211-1 can significantly improve feed to eggs conversion ratio. TLRI 211-1 supplementation also can maintain eggs at their optimum quality level during storage. The study showed that B. subtilis TLRI 211-1 can be used as feed additives for improving egg production performance and egg quality.
Keywords: Bacillus subtilis, Egg Quality, Egg Production Performance, Leghorn Layer


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