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https://doi.org/10.5713/ab.22.0389    [Accepted] Published online November 14, 2022.
Reproductive technologies needed for the generation of precise gene-edited pigs in the pathways from laboratory to farm
Ching-Fu Tu1,*  , Shu-Hui Peng1  , Chin-kai Chuang1  , Chi-Hong Wong1  , Tien-Shuh Yang1,* 
1Division of Animal Technology, Animal Technology Research Center, Agricultural Technology Research Institute, No.1, Ln. 51, Dahu Rd., Xiangshan Dist., Hsinchu City 30093, Taiwan
2Department of Biotechnology and Animal Science, National Ilan University, No.1, Sec. 1, Shennong Rd., Yilan City, Yilan County 260, Taiwan
Correspondence:  Ching-Fu Tu, Tel: +886-37-585815,, Fax: +886-37-585830, Email: cftu@mail.atri.org.tw
Tien-Shuh Yang,Email: tsy585960@gmail.com
Received: 11 October 2022   • Revised: 31 October 2022   • Accepted: 7 November 2022
Abstract
Abstract: Gene editing (GE) offers a new breeding technique (NBT) of sustainable value to animal agriculture. There are 3 GE working sites covering 5 feasible pathways to generate GE pigs along with the crucial intervals of GE/genotyping, microinjection/electroporation, induced pluripotent stem cells (iPSCs), somatic cell nuclear transfer (SCNT), cryopreservation, and nonsurgical embryo transfer (NSET). The extension of NBT in the new era of pig breeding depends on the synergistic effect of GE and reproductive biotechnologies; the outcome relies not only on scientific due diligence and operational excellence but also on the feasibility of application on farms to improve sustainability.
Keywords: Farm; Gene-editing; New Breeding Technique; Pigs; Reproductive Biotechnology; Sustainability
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