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https://doi.org/10.5713/ab.22.0277    [Accepted] Published online November 14, 2022.
Genome‑wide identification, organization, and expression profiles of the chicken fibroblast growth factor genes in public databases and Vietnamese indigenous Ri chickens against highly pathogenic avian influenza H5N1 virus infection
Anh Duc Truong2  , Ha Thi Thanh Tran2  , Nhu Thi Chu2  , Huyen Thi Nguyen2, Hao Thi Vu1  , Yeojin Hong1  , Ki-Duk Song3  , Hoang Vu Dang2,*  , Yeong Ho Hong1,* 
1Department of Biochemistry and Immunology, National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Ha Noi 100000, Vietnam
2The Animal Molecular Genetics and Breeding Center & Department of Animal Biotechnology, JeonBuk National University, Jeonju 54896, Korea
3Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea
Correspondence:  Hoang Vu Dang,Email: dangvuhoang@nivr.gov.vn
Yeong Ho Hong, Tel: +82-031-670-3025, Fax: +82-031-675-3108, Email: yhong@cau.ac.kr
Received: 16 July 2022   • Revised: 8 September 2022   • Accepted: 16 September 2022
Abstract
Objective
Fibroblast growth factors (FGFs) play critical roles in embryo development, and immune responses to infectious diseases. In this study, to investigate the roles of FGFs, we performed genome-wide identification, expression, and functional analyses of FGF family members in chickens.
Methods
Chicken FGFs genes were identified and analyzed by using bioinformatics approach. Expression profiles and Hierarchical cluster analysis of the FGFs genes in different chicken tissues were obtained from the genome-wide RNA-seq.
Results
A total of 20 FGF genes were identified in the chicken genome, which were classified into seven distinct groups (A–F) in the phylogenetic tree. Gene structure analysis revealed that members of the same clade had the same or similar exon-intron structure. Chromosome mapping suggested that FGF genes were widely dispersed across the chicken genome and were located on chromosomes 1, 4–6, 9–10, 13, 15, 28, and Z. In addition, the interactions among FGF proteins and between FGFs and MAPK proteins are limited, indicating that the remaining functions of FGF proteins should be further investigated in chickens. KEGG pathway analysis showed that FGF gene interacts with MAPK genes and are involved in stimulating signaling pathway and regulating immune responses. Furthermore, this study identified 15 differentially expressed genes (DEG) in 21 different growth stages during early chicken embryo development. RNA-sequencing data identified the DEG of FGFs on 1- and 3-days post infection in two indigenous Ri chicken lines infected with the highly pathogenic avian influenza virus H5N1 (HPAIV). Finally, all the genes examined through qRT-PCR and RNA-Seq analyses showed similar responses to HPAIV infection in indigenous Ri chicken lines (R2 = 0.92–0.95, p < 0.01).
Conclusion
This study provides significant insights into the potential functions of FGFs in chickens, including the regulation of MAPK signaling pathways and the immune response of chickens to HPAIV infections.
Keywords: Chicken; Expression Profile; Fibroblast Growth Factor; Genome-wide Analysis
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