Anim Biosci > Volume 36(3); 2023 > Article |
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Item | Hydrochloric acid (d) | Distilled water (d) | SEM | p-value3) | ||||||||||||||
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0 | 2 | 4 | 6 | 8 | 10 | 0 | 2 | 4 | 6 | 8 | 10 | HCl | L | Q | HCl×L | HCl×Q | ||
Urine weight (g) | 248 | 238 | 231 | 223 | 214 | 199 | 251 | 237 | 228 | 222 | 211 | 203 | 2 | 0.868 | <0.001 | 0.970 | 0.927 | 0.014 |
Nitrogen concentration (%) | 0.574 | 0.602 | 0.626 | 0.643 | 0.668 | 0.707 | 0.563 | 0.478 | 0.442 | 0.403 | 0.366 | 0.326 | 0.071 | <0.001 | 0.002 | 0.198 | <0.001 | 0.451 |
Nitrogen amount (g) | 1.43 | 1.44 | 1.45 | 1.43 | 1.43 | 1.40 | 1.41 | 1.13 | 1.01 | 0.90 | 0.77 | 0.66 | 0.16 | <0.001 | <0.001 | 0.234 | <0.001 | 0.051 |
Corrected nitrogen4) (%) | 0.574 | 0.571 | 0.575 | 0.574 | 0.572 | 0.571 | 0.563 | 0.452 | 0.404 | 0.359 | 0.311 | 0.265 | 0.064 | <0.001 | <0.001 | 0.124 | <0.001 | 0.130 |
GE concentration (kcal/kg) | 70.8 | 77.2 | 74.1 | 77.7 | 83.7 | 92.4 | 48.3 | 45.7 | 42.6 | 44.2 | 46.5 | 48.9 | 7.3 | <0.001 | <0.001 | 0.197 | 0.001 | 0.800 |
GE amount (kcal) | 17.6 | 18.4 | 17.1 | 17.3 | 18.0 | 18.4 | 12.2 | 10.8 | 9.7 | 9.8 | 9.8 | 9.9 | 1.6 | <0.001 | 0.199 | 0.399 | 0.053 | 0.363 |
Corrected GE5) (kcal/kg) | 70.8 | 73.2 | 68.1 | 69.3 | 71.7 | 74.6 | 48.3 | 43.2 | 39.0 | 39.4 | 39.6 | 39.7 | 6.6 | <0.001 | 0.259 | 0.276 | 0.042 | 0.564 |
3) HCl, supplementation of 6 N HCl in urine; L, linear effects of day; Q, quadratic effects of day; HCl×L, the interaction between supplementation of 6 N HCl in urine and linear effects of day; HCl×Q, the interaction between supplementation of 6 N HCl in urine and quadratic effects of day.
BW (kg) | Number of observations | Maximum nitrogen (g/d) | Minimum nitrogen (g/d) | Mean (g/d) | Required 6 N HCl2) (mL/d) |
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0 to 20 | 88 | 12.6 | 2.1 | 4.6 | 48 |
20 to 40 | 101 | 29.6 | 2.8 | 15.1 | 113 |
40 to 60 | 270 | 49.6 | 5.3 | 20.0 | 190 |
60 to 80 | 225 | 59.1 | 7.0 | 23.2 | 227 |
80 to 110 | 121 | 62.7 | 7.1 | 27.9 | 240 |
1) Data were from 9 published experiments and 8 unpublished experiments that measured daily urinary nitrogen excretion.
2) The amount of 6 N HCl required for urinary nitrogen preservation was calculated by multiplying the maximum quantity of daily nitrogen excretion for each BW range with the required amount of 6 N HCl for nitrogen preservation per gram of urinary nitrogen (3.83 mL/g) which is the slope in Figure 3.