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https://doi.org/10.5713/ab.21.0424    [Accepted] Published online January 5, 2022.
In vitro fermentation profiles of different soybean oligosaccharides and their effects on skatole production and cecal microbiota of broilers
Xin Zhu1  , Miao Xu1  , Haiying Liu1  , Guiqin Yang1,* 
College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China
Correspondence:  Guiqin Yang,Email: yguiqin@syau.edu.cn
Received: 16 September 2021   • Revised: 10 November 2021   • Accepted: 22 December 2021
Abstract
Objective
The objective of this study was to investigate the in vitro fermentation profiles of different soybean oligosaccharides and their effects on skatole production and cecal microbiota of broilers.
Methods
Five soybean oligosaccharides (SBOs) with varying main component contents were fermented using an in vitro batch incubation inoculated with broiler cecal microbiota. Gas production was recorded automatically, skatole, indole and short-chain fatty acids (SCFAs) were determined using high-performance liquid chromatography (HPLC), and microbial changes were analyzed using 16S DNA gene sequencing.
Results
The addition of SBOs increased (P < 0.05) gas production, suggesting bacterial growth-stimulating activities. In addition, the concentrations of indole were significantly (P < 0.05) decreased after SBO supplementation, and SBO III, with higher sucrose and stachyose contents, decreased (P < 0.05) the skatole level. Our results also revealed that the fermentation of SBOs by cecal microbiota produced (P < 0.05) SCFAs, which were dominated by propionic acid, butyrate acid and lactic acid compared to the control. In addition, SBO III increased (P < 0.05) the abundance of Firmicutes and Subdoligranulum and decreased that of Bacteroides.

Conclusion

These results suggest that SBOs with higher sucrose and stachyose contents are promising prebiotics in modulating gut microbiota and reducing odor emission in broilers.
Keywords: Broilers; Gut Microbiota; Odors; Oligosaccharides; Short Chain Fatty Acids
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