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https://doi.org/10.5713/ab.21.0430    [Accepted] Published online January 5, 2022.
Extraction of dietary fibers from cassava pulp and cassava distiller’s dried grains and assessment of their components using Fourier Transform Infrared Spectroscopy to determine on their further use as a functional feed in animal diets
Supattra Okrathok1  , Kanjana Thumanu2  , Chayanan Pukkung1  , Wittawat Molee1  , Sutisa Khempaka1,* 
1School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
2Synchrotron Light Research Institute (Public Organization), Nakhon Ratchasima, 30000, Thailand
Correspondence:  Sutisa Khempaka, Tel: +66-44224378, Fax: +66-44224376, Email: khampaka@sut.ac.th
Received: 20 September 2021   • Revised: 4 November 2021   • Accepted: 12 December 2021
The present study was to investigate the extraction conditions of dietary fiber from dried cassava pulp (DCP) and cassava distiller’s dried grains (CDG) under different NaOH concentrations, and the Fourier Transform Infrared (FTIR) was used to determine the dietary fiber components.
The dried samples (DCP and CDG) were treated with various concentrations of NaOH at levels of 2, 4, 6, and 8% using a completely randomized design with 4 replications of each. After extraction, the residual DCP and CDG dietary fiber were dried in a hot air oven at 55–60 ºC. Finally, the oven dried extracted dietary fiber was powdered to a particle size of 1 mm. Both extracted dietary fibers were analyzed for their chemical composition and determined by FTIR.
The DCP and CDG treated with NaOH linearly or quadratically or cubically (p<0.05) increased the total dietary fiber (TDF) and insoluble fiber (IDF). The optimal conditions for extracting dietary fiber from DCP and CDG were under treatment with 6% and 4% NaOH, respectively, as these conditions yielded the highest TDF and IDF contents. These results were associated with the FTIR spectra integration for a semi-quantitative analysis, which obtained the highest cellulose content in dietary fiber extracted from DCP and CDG with 6% and 4% NaOH solution, respectively. The Principal Component Analysis (PCA) illustrated clear separation of spectral distribution in cassava pulp extracted dietary fiber (DFCP) and cassava distiller’s dried grains extracted dietary fiber (DFCDG) when treated with 6% and 4% NaOH, respectively.


The optimal conditions for the extraction of dietary fiber from DCP and CDG were treatment with 6% and 4% NaOH solution, respectively. In addition, FTIR spectroscopy proved itself to be a powerful tool for fiber identification.
Keywords: Dietary Fiber; FTIR Spectroscopy; Cassava Pulp; Cassava Distiller’s Dried Grains
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