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Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.21.0270    [Accepted] Published online September 15, 2021.
Effect of lactic acid bacteria and yeast supplementation on anti-nutritional factors and chemical composition of fermented total mixed ration containing cottonseed meal or rapeseed meal
Hassan Ali Yusuf1,2  , Minyu PIAO1  , Tao MA1  , Ruiying HUO1  , Yan TU1,* 
1Institute of Feed Research of Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, 100193 Beijing, China
2Faculty of Veterinary Medicine and Animal Husbandry, Somali National University, P.O Box 15 Mogadishu, Somali
Correspondence:  Yan TU,Email: tuyan@caas.cn
Received: 13 June 2021   • Revised: 28 July 2021   • Accepted: 5 September 2021
This study aimed to determine the appropriate supplementation level of lactic acid bacteria (LAB; Lactobacillus plantarum and Bacillus clausii), yeast (Saccharomyces cariocanus and Wickerhamomyces anomalus) for degrading free gossypol and glucosinolate in the fermented total mixed ration (TMR) containing cottonseed meal (CSM) or rapeseed meal (RSM), to improve the utilization efficiency of these protein sources.
For LAB, Lactobacillus plantarum or Bacillus clausii was inoculated at 1.0 × 108, 1.0 × 109, 1.0 × 1010, and 1.0 × 1011 CFU/kg DM, respectively. For yeast, Saccharomyces cariocanus or Wickerhamomyces anomalus was inoculated at 5 × 106, 5 × 107, 5 × 108, and 5 × 109 CFU/kg DM, respectively. The TMR had 50% moisture and was incubated at 30°C for 48 h. After fermentation, the chemical compositions, and the contents of free gossypol and glucosinolate were determined.
The results showed that the concentration of free gossypol content was reduced (P<0.05), while that of the crude protein content was increased (P < 0.05) in the TMR containing CSM inoculated by B. clausii (1 × 109 CFU/kg DM) or S. cariocanus (5 × 109 CFU/kg DM). Similarly, the content of glucosinolate was lowered (P < 0.05) and the crude protein content was increased (P < 0.05) in TMR containing RSM inoculated with B. clausii (1 × 1010 CFU/kg DM) or S. cariocanus (5 × 109 CFU/g DM).


This study confirmed that inclusion of B. clausii with 1.0 × 109 or 1.0 × 1010 CFU/kg DM, or S. cariocanus (5 × 109 CFU/kg DM) to TMR containing CSM/RSM improved the nutritional value and decreased the contents of anti-nutritional factors.
Keywords: Cottonseed Meal; Rapeseed Meal; Total Mixed Ration; Lactic Acid Bacteria; Yeast; Free Gossypol; Glucosinolates

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