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Anim Biosci > Volume 34(4); 2021 > Article
Nonruminant Nutrition and Feed Processing
Animal Bioscience 2021;34(4): 662-669.
DOI: https://doi.org/10.5713/ajas.20.0030    Published online June 23, 2020.
Linseed oil supplementation affects fatty acid desaturase 2, peroxisome proliferator activated receptor gamma, and insulin-like growth factor 1 gene expression in turkeys (Meleagris gallopavo)
Klaudia Szalai1,*  , Károly Tempfli1  , Eszter Zsédely1  , Erika Lakatos2  , András Gáspárdy3  , Ágnes Bali Papp1 
1Department of Animal Science, Faculty of Agricultural and Food Sciences, Széchenyi István University, 9200 Mosonmagyaróvár, Hungary
2Department of Food Science, Faculty of Agricultural and Food Sciences, Széchenyi István University, 9200 Mosonmagyaróvár, Hungary
3Department of Animal Breeding and Genetics, University of Veterinary Medicine, 1078 Budapest, Hungary
Correspondence:  Klaudia Szalai, Tel: +36-96-566-713, Fax: +36-96-566-620, Email: szalai.klaudia@sze.hu
Received: 15 January 2020   • Revised: 26 March 2020   • Accepted: 17 May 2020
Abstract
Objective
Effects of linseed oil (LO) supplementation on the fat content and fatty acid profile of breast meat, and the expression of three genes in the liver, breast muscle and fat tissues of commercial 154-day-old hybrid male turkeys were investigated.
Methods
The animals in the control group were fed a commercially available feed and received no LO supplementation (n = 70), whereas animals in the LO group (n = 70) were fed the same basic diet supplemented with LO (day 15 to 21, 0.5%; day 22 to 112, 1%). The effect of dietary LO supplementation on fatty acid composition of breast muscle was examined by gas chromatography, and the expression of fatty acid desaturase 2 (FADS2), peroxisome proliferator activated receptor gamma (PPARγ), and insulin-like growth factor 1 (IGF1) genes was analysed by means of quantitative reverse transcription polymerase chain reaction.
Results
The LO supplementation affected the fatty acid composition of breast muscle. Hepatic FADS2 levels were considerably lower (p<0.001), while adipose tissue expression was higher (p<0.05) in the control compared to the LO group. The PPARγ expression was lower (p<0.05), whereas IGF1 was higher (p<0.05) in the fat of control animals. There were no significant (p>0.05) differences in FADS2, PPARγ, and IGF1 gene expressions of breast muscle; however, omega-6/omega-3 ratio of breast muscle substantially decreased (p<0.001) in the LO group compared to control.
Conclusion
Fatty acid composition of breast meat was positively influenced by LO supplementation without deterioration of fattening parameters. Remarkably, increased FADS2 expression in the liver of LO supplemented animals was associated with a significantly decreased omega-6/omega-3 ratio, providing a potentially healthier meat product for human consumption. Increased PPARγ expression in fat tissue of the LO group was not associated with fat content of muscle, whereas a decreased IGF1 expression in fat tissue was associated with a trend of decreasing fat content in muscle of the experimental LO group.
Keywords: Fatty Acid Desaturase 2 (FADS2); Gene Expression; Insulin-like Growth Factor 1 (IGF1); Meleagris gallopavo; Peroxisome Proliferator Activated Receptor Gamma (PPARγ)
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