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Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 2009;22(2): 168-173.
https://doi.org/10.5713/ajas.2009.80225    Published online January 6, 2009.
Production of Bovine Nuclear Transfer Embryos Using Fibroblasts Transfected with Single-Chain Human Follicle-Stimulating Hormone Gene
Ji Young Yoon, Mo Sun Kwon, Jee Hyun Kang, Kwang Sung Ahn, So Seob Kim, Nam-Hyung Kim, Jin-Hoi Kim, Teoan Kim, Hosup Shim*
Correspondence:  Hosup Shim,
Human follicle-stimulating hormone (hFSH) is a pituitary glycoprotein that regulates follicular development and ovulation. Clinically, hFSH has been used to induce follicular growth in infertile women. The hormone is composed of heterodimers, including a common  subunit among the gonadotropin family and a hormone-specific  subunit. Since assembly of the heterodimer is a rate-limiting step in the production of functional hFSH, transgenic clone cows carrying a single-chain hFSH transgene may efficiently produce functional hormone. Genes encoding the  and  subunits of hFSH were linked using the C-terminal peptide sequence from the  subunit of human chorionic gonadotropin. Bovine fetal fibroblasts were transfected with the gene construct, including the goat -casein promoter and a single-chain hFSH coding sequence. Transfected fibroblasts were transferred into enucleated oocytes, and individual nuclear transfer (NT) embryos developed to the blastocyst stage were analyzed for the transgene by polymerase chain reaction. Seventy eight blastocysts (30.8%) were developed from 259 reconstructed embryos. Among these blastocysts, the hFSH gene was detected in 70.8% (34/48) of the embryos. Subsequent transfer of hFSH-transgenic clone embryos to 31 recipients results in 11 (35.5%) early pregnancies. However, all fetuses were lost before reaching day 180 of gestation. The results from this study demonstrated that bovine NT embryos carrying single-chain hFSH could be produced, and further extensive studies in which NT embryos are transferred to more recipients may give rise to single chain hFSH-transgenic cows for biomedical applications.
Keywords: Single-chain hFSH; Transgenic; Nuclear Transfer; Cow

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