Go to Top Go to Bottom
Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 2009;22(1): 26-34.
https://doi.org/10.5713/ajas.2009.80343    Published online January 6, 2009.
Efficient Derivation and Long Term Maintenance of Pluripotent Porcine Embryonic Stem-like Cells
Hye-Young Son, Jung-Eun Kim, Sang-Goo Lee, Hye-Sun Kim, Eugene Lee, Jin-Kyu Park, Hakhyun Ka, Hyun-Jong Kim, Chang-Kyu Lee*
Correspondence:  Chang-Kyu Lee,
Porcine embryonic stem (ES) cells have a great potential as tools for transgenic animal production and studies of regulation of differentiation genes. Although several studies showed successful derivation of porcine ES-like cells, these cells were not maintained long-term in culture. Therefore, this study was conducted to establish porcine pluripotent ES-like cells using in vivo fertilized embryos and to maintain these cells in long term culture. Porcine ES-like cells from in vivo embryos obtained by immuno-surgery or whole explant culture were successfully cultured for over 56 passages. Morphology of porcine ES-like cells was flat-shaped with a monolayer type colony. These cells stained for alkaline phosphatase throughout the culture. Furthermore, porcine ES-like cells reacted with antibodies against Oct-4, SSEA-1, SSEA-4, Tra-1-60, and Tra-1-81, which are typical markers of undifferentiated stem cells. To characterize the ability of porcine ES-like cells to differentiate into three germ layers, embryoid body formation was induced. After plating of these cells, porcine ES-like cells were spontaneously differentiated into various cell types of all three germ layers. In addition, porcine ES-like cells were successfully derived from IVF blastocysts in media containing human recombinant basic fibroblast growth factor.
Keywords: Porcine; Embryonic Stem Cells; Pluripotent; IVF; Basic Fibroblast Growth Factor
METRICS Graph View
  • 7 Crossref
  • 7 Scopus
  • 3,884 View
  • 40 Download
Related articles

Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul 08776, Korea   
TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : editor@animbiosci.org               

Copyright © 2024 by Asian-Australasian Association of Animal Production Societies.

Developed in M2PI

Close layer
prev next