Anti-idiotypic Antibodies against Bovine Growth Hormone

Verma, N. K.; Sodhi, R.; Rajput, Y. S.

doi:2003.16.5.732

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 2003;16(5): 732-737.
https://doi.org/10.5713/ajas.2003.732 Published online January 1, 2003.

Anti-idiotypic Antibodies against Bovine Growth Hormone

N. K. Verma, R. Sodhi, Y. S. Rajput

Abstract

Anti-antibodies against three mouse monoclonal antibodies viz. IIB5D6, VIA6E8 and VIC1F9 (specific to bovine growth hormone) in rabbits have been generated and characterized. Ammonium sulfate fractionated and affinity-purified monoclonal antibodies were used for producing anti-antibodies. The generated anti-antibodies were against common as well as uncommon antigenic determinants present in mouse monoclonal antibodies. The raised anti-antibodies replaced [ I125 ]bGH bound to goat liver microsomes indicating production of anti-idiotypic antibodies against bovine growth hormone. These antibodies can have profound implications in vivo in lactating bovines for enhancing milk yield.

Keywords: Anti-idiotypic Antibodies; Bovine Growth Hormone; Bovine Somatotropin; Receptor Assay; Antibody Purification