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Ruminant Nutrition and Forage Utilization
Asian-Australasian Journal of Animal Sciences 1996;9(3): 299-302.
https://doi.org/10.5713/ajas.1996.299    Published online June 1, 1996.
Studies on the early pregnancy determination in cows by using the enzyme-immunoassy and radio-immunoassay in milk
J. M. Lee, H. S. Kim, S. G. Jeong, J. K. Jung
Milk samples(n = 78) were taken 19d, 20d, 21d, 22d after artificial insemination(AI) for early pregnancy diagnosis by using the Enzyme immunoassay(EIA) kit. The progesterone(P4) concentration in the whole milk was measured on the same day of pregnancy diagnosis. Rectal palpation(RP) was accomplished between 60d and 70d after AI to estimate the ovary condition and pregnancy status. Milk progesterone concentrations measured by Radio-immunoassay(RIA) method, in the pregnant cows at 17d, 19d, 21d after insemination were 17.10 0.91, 17.60 0.46 and 18.43 0.79 nmol/l, whereas those in the not-pregnant cows were 6.57 1.03, 2.63 0.29 and 0.67 0.08 nmol/l, respectively. When the progesterone concentration was less than 7 nmol/l, the color of the EIA kit was lighter and when the progesterone concentration was > 16 nmol/l, the color of the EIA kit was darker compared to the standard color. The detection rates of error by judging the color differences were 5.1% and 20.7%, respectively. In the early pregnancy diagnosis by the EIA kit and RIA method, the accuracy rates in the pregnancy of cows were 82% and 87%, and those in not-pregnant cows were 86% and 91%, respectively. For ovarian status estimated by the RIA method and certified by RP, the accuracy rates of the ovarian atrophy, follicular cyst and luteal cyst were 80, 91 and 83% and the progesterone concentrations were 2.51, 2.03 and 26.7 nmol/l, respectively.
Keywords: Milk Progesterone; Pregnancy Diagnosis; Ovarian Status; EIA; RIA
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