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Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 1995;8(4): 317-320.
DOI: https://doi.org/10.5713/ajas.1995.317    Published online August 1, 1995.
Effect of granulosa and cumulus cells on in vitro development of the bovine follicular oocytes
K. S. Im, H. J. Kim, K. M. Chung, H. S. Kim, K. W. Park, K. Niwa
The effect of co-culture with cumulus cells and granulose cells during maturation and development on in vitro developmental potency of follicular oocytes was examined. TCM-199 supplemented with 15% FCS and hormones was used as maturation medium. Sperm from frozen semen was capaciated in modified mTALP medium containing 0.3% BSA, 10 關g/ml heparin and 5 mM/ml caffeine. The fertilized embryos were co-cultured on monolayer of cumulus cells or granulose cells in TCM-199. The embryo co-cultured with cumulus cells showed higher percentage of embryo developed to morula and blastocyst(73.3%) than the embryo co-cultured without cumulus cells(30.8%). The percentage of oocytes developed to morula and blastocyst among cleaved oocytes was significantly(p<0.05) higher in the oocytes co-cultured with cululus cells during development(62.4%) than in the oocytes co-cultured with granulose cells during maturation and with cumulus cells during development(52.3%), and in the oocytes co-cultured with granulose cells during development(52.8%). The results of this study indicate that co-culture of in vitro fertilized embryos with cumulus cells in the development medium increased the rate of embryos developed to morula and blastocyst among cleaved oocytes.
Keywords: Follicular Oocyte; Co-culture; Cumulus Cells; Granulosa Cells; Blastocyst

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