Influence of Sunflower Whole Seeds or Oil on Ruminal Fermentation, Milk Production, Composition, and Fatty Acid Profile in Lactating Goats

Article information

Asian-Australas J Anim Sci. 2015;28(8):1116-1122
1Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, C.P. 50000, Estado de México, México.
2Animal Production Research Institute, Agriculture Research Center, Dokki, Cairo, 12618, Egypt.
*Corresponding Author: A. Z. M. Salem Tel: +20-521-722-296-55-42, Fax: +20-521-722-180-61-94, E-mail:
Dairy Science Department, National Research Center, Dokki, Giza, 12311, Egypt
Received 2014 November 03; Revised 2015 February 02; Accepted 2015 February 11.


This study aimed to investigate the effect of sunflower seeds, either as whole or as oil, on rumen fermentation, milk production, milk composition and fatty acids profile in dairy goats. Fifteen lactating Damascus goats were divided randomly into three groups (n = 5) fed a basal diet of concentrate feed mixture and fresh Trifolium alexandrinum at 50:50 on dry matter basis (Control) in addition to 50 g/head/d sunflower seeds whole (SS) or 20 mL/head/d sunflower seeds oil (SO) in a complete randomized design. Milk was sampled every two weeks during 90 days of experimental period for chemical analysis and rumen was sampled at 30, 60, and 90 days of the experiment for ruminal pH, volatile fatty acids (tVFA), and ammonia-N determination. Addition of SO decreased (p = 0.017) ruminal pH, whereas SO and SS increased tVFA (p<0.001) and acetate (p = 0.034) concentrations. Serum glucose increased (p = 0.013) in SO and SS goats vs Control. The SO and SS treated goats had improved milk yield (p = 0.007) and milk fat content (p = 0.002). Moreover, SO increased milk lactose content (p = 0.048) and feed efficiency (p = 0.046) compared to Control. Both of SS and SO increased (p<0.05) milk unsaturated fatty acids content specially conjugated linolenic acid (CLA) vs Control. Addition of SS and SO increased (p = 0. 021) C18:3N3 fatty acid compared to Control diet. Data suggested that addition of either SS or SO to lactating goats ration had beneficial effects on milk yield and milk composition with enhancing milk content of healthy fatty acids (CLA and omega 3), without detrimental effects on animal performance.


Goat milk has been identified as an alternative for human consumers who are either sensitive or allergic to cow’s milk (Dixit et al., 2012). Lipid composition is one of the most important components of the nutritional quality of goat milk. The nutritional advantage of goat milk fat compared to cow’s milk may been attributed to the high content of C6:0 to C10:0 fatty acids (FA), the high percentage of the short- and medium-chain FA, and to the small size of fat globules; hence making goat’s dairy product easily digestible than cow’s dairy product (Chilliard et al., 2006). However, goat milk contains a high proportion of saturated FA (SFA); the consumption of which is considered a risk factor for human cardiovascular disease (Ney, 1991). Ruminant milk FA composition can be changed significantly in response to feeding regimen, especially lipid supplementation, which has variable effects depending on the lipid added and its interaction with the type and proportion of forage and concentrate in the diet (Chilliard et al., 2007; Kholif et al., 2014).

Addition of seeds oil to lactating dairy cows ration was one of the methods to change the proportion of total unsaturated FA (TUFA) in milk fat by about 40%, although extensive biohydrogenation occurs normally in the rumen (Dschaak et al., 2009). Sunflower whole seeds would be a good choice from a consumer point of view, as it is rich in polyunsaturated FA (PUFA). Sunflowers being a source of linoleic acid (66% of total FA), which is an n-6 FA (Ebrahimi et al., 2014). Whole seeds of sunflowers do not have antinutritional factors such as those found in the other major oilseeds, including soybeans, cottonseeds and rapeseeds. For this reason, it is considered to be a safe feed ingredient for all species.

Due to high oil content, sunflower seeds are used as an energy source. However, they are particularly valued if used as a supplement for the dietary management of the FA profile of dairy and meat products. This may be since sunflower seeds whole (SS) and sunflower seeds oil (SO) are rich in PUFA and conjugated linoleic acid (CLA), which have proven beneficial effects on human health (Peng et al., 2010). Feeding SS resulted an increase of CLA, C18:2 and omega-6 content in milk and meat of ruminant animals (Ebrahimi et al., 2014). Beauchemin et al. (2009) noted increased lag time of fiber degradation in the rumen and in methane emissions reduction with sunflower seeds as a feed additive. Seeds of sunflower can improve rumen bacterial activity and increase total bacteria and subsequent bacterial amino acid flow through reducing the rumen protozoa and rumen bacteria predators (Ivan et al., 2004). Therefore, the objective of this study was to evaluate the impact of SS or SO on lactating Damascus goat’s milk yield and CLA content, in order to develop a practical feeding strategies for goat production systems.


Study location

The present study was carried out at the dairy science department, National Research Centre, Egypt and the experimental farm of Animal Production Research Institute, Gemazza, Egypt.

Animals housing and feeding

Fifteen lactating Damascus goats with an average live body weight of 44.8 kg and in the 3rd or 4th lactating seasons were randomly divided into three equal and similar groups to be fed a basal diet individually.

Goats were fed a basal diet (concentrate feed mixture [CFM]) and fresh Trifolium alexandrinum (1:1, on dry matter [DM] bases) of 142.0 g/kg crude protein (CP) and 142.0 g/kg crude fiber (CF). Three treatments (5 goats/treatment) were (Control) (basal diet with no additive; the same control treatment of Kholif et al., 2015), SS (Control plus a daily dose of 50 g/head/d of SS) or SO (Control plus a daily dose of 20 mL/head/d of SO). The additives (i.e., whole seeds or oil) were mixed with about 100 g of the concentrates before morning feeding at 08.00 h and fed to goats. The offered feeds were assessed to cover the maintenance and production requirements for each goat (NRC, 2007). Chemical composition of CFM and Trifolium alexandrinum is shown in Table 1. The CFM was offered individually for each animal once daily at 08.00 h, while fresh Trifolium alexandrinum was offered at 10.00 and 16.00 h for 90 days. Dry matter intake was recorded daily throughout the experimental period by weighing the offered diets and refusals from the previous day. Drinking water was available at all time.

Chemical composition (%) of the concentrate feed mixture (CFM1), fresh Trifolium alexandrinum, sunflower seeds whole2 or oil3 and the basal diet4 (on DM basis)

Samples of feed ingredient were analyzed for DM (ID 930.15), ash (ID 942.05), CP (ID 984.13), CF (ID 962.09) and ether extract (ID 945.16) according to methods of AOAC (1997). Nitrogen-free extract and organic matter were calculated by difference.

Samples of SS and oil were analyzed for FA content according to the method of AOAC (2007) using FA methyl esters prepared by base-catalyzed methanolysis of the glycerides (KOH in methanol). On a Perkin-Elmer chromatograph (model 8420, Beaconsfield, Perkin Elmer, Beaconsfield, UK) equipped with a flame ionization detector, the FA were separated. Helium was the carrier gas at 30 cm/sec, where column temperature was initially 150°C for 1 min, and then increased by 4°C/min to 200°C, and was then held at 200°C for 10 min. Individual FA methyl esters were identified by retention time with reference to methyl esters standards.

Sampling and analysis of rumen liquor

Rumen liquor samples were collected from three animals within each group by a stomach tube. Collection was performed four hours after morning feeding of concentrates, after 30, 60, and 90 days of the experiment. Rumen samples were filtered through four layers of cloth and used quickly as possible for the measurement of pH using a digital pH-meter. Strained rumen liquor was stored in glass bottles (25 mL) with few drops of toluene and paraffin oil just to cover the surface, and stored at a deep freeze (−18°C) till chemical analysis. The concentration of ruminal ammonia-N was determined according to AOAC (2007). Ruminal total volatile FA (tVFA) and fractions of volatile fatty acids were determined by gas chromatography (Varian 3700; Varian Specialties Ltd, Brockville, ON, Canada).

Sampling and analysis of blood serum

Blood samples were withdrawn from the jugular vein of the same three animals which used for rumen liquor collection, after 30, 60, and 90 days after four hours of concentrates morning feeding. The collected blood samples were centrifuged at 4,000×g at 4°C for 20 min. Serum was separated into clean dried glass vials and frozen at −20°C till analysis. Blood serum samples were analyzed for concentrations of total protein, albumin, glucose, cholesterol and triglycerides using specific kits (Stanbio Laboratory, Boerne, TX, USA) as manufacturer instructions. Globulin concentration and albumin/globulin ratio (A/G ratio) were calculated.

Sampling and milk composition and fatty acids analysis

Individual milk samples were collected from all goats every two weeks during the experimental period (i.e., 90 days). Goats were hand milked twice daily at 08:00 and 16:00 h and the milk yield was recorded. The sample of each animal represents a mixed sample of constant percentage of the evening and morning yield. Milk samples were analyzed for total solids, fat, protein, non-protein N and lactose using infrared spectrophotometry (Foss 120 Milko-Scan, Foss Electric, Hillerød, Denmark). The ash content of milk was determined after heating milk sample in a muffle furnace at 550°C for 16 h, and the solids not fat content was calculated by difference. Fatty acids in milk were determined as described previously in Kholif et al. (2014) using methyl esters prepared by base-catalyzed methanolysis of the glycerides (KOH in methanol) according to International Standards (ISO-IDF, 2002). Fatty acid methyl esters were separated using a Cp-Sil 88 fused-silica capillary column (100 m×0.25 mm i.d.×0.2 μm film thickness, Chrompack, Middelburg, Netherlands) on a Perkin-Elmer chromatograph (model 8420, Beaconsfield, Perkin Elmer, Beaconsfield, UK) equipped with a flame ionization detector.

Statistical analysis

Statistical analysis for the obtained data was carried out using the general linear model producers of SAS (SAS Inst. Inc. Cary, NC, 2006) in a complete randomized design using the following model:


Where Yij expressed every observation of the jth animal in the ith treatment, μ expressed the general mean, Ti expressed the treatment effect, and Eij expressed the experimental error. Comparison among means was carried out using Duncan’s Multiple Range Test (Duncan, 1955). Significance was declared at a level of p<0.05 and trend of p≤0.10 (Steel and Torrie, 1980).


Dry matter intake and rumen activity

Live body weight of goats was not different (p>0.05) among experimental groups which reflects the random distribution of goats into the treatments.

Goats of all experimental groups consumed approximately (p>0.05) the same amount of feeds during the experimental period. These results were similar to that obtained by Petit et al. (2004) and Mohammed et al. (2011) who not found different in dry matter intake (DMI) among treatments have been reported in lactating cows fed whole, unprocessed sunflower and flaxseed seeds. Moreover, feeding sunflower whole seeds at 10% of cow’s diet had no effect on DMI (Rafalowski and Park, 1982). However, greater DMI for cows fed whole flaxseed compared with those fed whole sunflower seeds have been previously reported (Petit, 2003).

Decreased ruminal pH values were occurred (p = 0.017) due to SO and SS addition compared to Control (Table 2). Ruminal pH values for all treatments ranged between 5.8 and 6.2, which were within the range considered acceptable for fiber digestion (Ørskov and Ryle, 1990). Decreased ruminal pH values with SS and SO addition may be association with increased energy density (Fulton et al., 1979) or the increased concentrations of tVFA in both treatments compared to Control goats (Baile and Forbes, 1974).

Goats weight, feed intake, and rumen fermentation kinetics of lactating Damascus goats fed basal diet with sunflower seeds whole (SS) or sunflower seeds oil (SO)

Increased tVFA (p<0.001) and acetate (p = 0.034) concentrations were obtained as a result of SO and SS addition to goats diet. No differences (p>0.05) were noted for propionate and butyrate concentrations among SS and SO or control goats. Total VFA concentration in the rumen depends on many factors including nutrient digestibility, rate of absorption, rumen pH, rate of digesta passage from rumen, as well as the microbial population in the rumen and their activities (Flatt et al., 1956). The higher concentrations of tVFA with SS or SO indicate the more efficient anaerobic fermentation, which may be due to increased organic matter and fibers digestibility (Khattab et al., 2011). The higher of acetate proportion of treated groups may be possibly resulted from an increased cellulolytic bacteria activity in rumen with rations contained SS or SO.

Decreased concentrations of ruminal ammonia-N concentration (p = 0.046) were occurred when SS or SO were added to goats diets (Table 2). Ruminal ammonia-N concentrations were ranged between 269 to 283 mg/L which were above the range (0.60 to 1.59 mM ammonia-N) considered by Satter and Slyter (1974) to be sufficient for microbial N production. Ammonia-N level depends on many factors, including the dietary CP solubility as the main one. Reduction of ammonia-N concentrations in the SS and SO goats was paralleled with increased ruminal tVFA which considered as an indicator of higher fermentation rate and increased microbial protein synthesis (Kholif et al., 2014). Moreover, decreased ammonia-N with SS and SO reflects increased ammonia consumed by ruminal bacteria for production of microbial protein in the rumen.

Blood biochemical parameters

Addition of SS and SO to goat diets did not alter (p>0.05) blood chemistry whereas serum glucose concentration was increased (p = 0.013) with the two additions in goats diets (Table 3). Increased serum glucose concentration with SS and SO may be due to increased tVFA concentration in the rumen of goats. It is of interest that blood serum glucose of the SS and SO groups followed the same trend as their milk yield, which may confirm results of Clark et al. (1977) who claimed a positive relationship between blood serum glucose and milk yield.

Blood serum parameters of lactating Damascus goats fed basal diet with sunflower seeds whole (SS) or sunflower seeds oil (SO)

Milk yield, composition and fatty acids profile

Increased milk yield (p = 0.007) and milk fat content (p = 0.002) was obtained as a result of SO and SS addition to goat diets. Moreover, increased lactose content (p = 0.048) with improved feed efficiency (milk yield/DMI; p = 0.046) was obtained with SO vs SS and control. No effects were occurred (p>0.05) for milk content of protein, total solids, solids not-fat, ash, and urea N as a result of SS and SO addition in the goat diets (Table 4).

Average daily milk yield and composition of lactating Damascus goats fed basal diet with sunflower seeds whole (SS) or sunflower seeds oil (SO)

Milk production was increased with SO and SS goats without increasing DMI; this phenomena reflects better utilization of feeds. The increased milk yield obtained with SS and SO diets may be due to the increased tVFA concentration in rumen of goats fed diets added with SS or SO, and apparent increased in the efficiency of N utilization, as well as an increased conversion and availability of nutrients for milk synthesis. The current results are in agreement with those of Abu Ghazaleh and Holmes (2007) and Castro et al. (2009) who reported that addition of sunflower seeds oil to cows ration increased milk yield. However, other studies reported a reduction in milk production by fed on sunflower whole seeds (Petit et al., 2004; Mohammed et al., 2011) or no change as a result of addition sunflower oil (Ollier et al., 2009) or sunflower whole seeds (Beauchemin et al., 2009).

Increased milk fat concentration for SS or SO goats could be related to more C2 produced in the rumen of goats fed diets with SS or SO addition. Increased milk lactose and fat contents with the SO addition may be due to the increased blood serum glucose concentration and increased rumen fermentation with SO goats (Ollier et al., 2009). In addition, Sanz Sampelayo et al. (2002) reported a high correlation coefficient (r = 0.95) between lactose production and fat supplementation in goats fed isocaloric diets.

Increased concentrations of milk C6:0, C8:0, C14:1, and C16:1, C18:1n9c FA and TUFA with decreasing C17:0 concentrations were obtained with SO goats. Moreover, both of SO and SS had increased C18:2 trans-10, cis-12, C18:3N3 and total CLA concentrations compared to Control (Table 5). Fatty acids can be originated from plasma (60%) or by the synthesis in the mammary gland from acetate and hydroxybutyrate originated from rumen fermentation involving acetyl CoA carboxylase enzymes and fatty acid synthetase (Mesquita et al., 2008; Kholif et al., 2014). Polyunsaturated FA are not synthesized by ruminants, so that their concentration in milk depends on the amount of PUFA absorbed from the intestines. Milk from goats receiving sunflower seeds or oil supplementation displayed an improvement in the FA profile, with an increased PUFA and monounsaturated FA contents and a decreased in SFA content. These results are in agreement with other studies (Castro et al., 2009; Mohammed et al., 2011), which reported similar changes in milk fat when sunflower seeds supplements were used. In the present study, the decreased C14:0 and C16:0 FA contents in milk from goats fed SS or SO may be a positive goal from a human health perspective, because high proportions of C14:0 and C16:0 FA has been associated with human cardiovascular problems (Noakes et al., 1996). Moreover, the increased C18:1 content with SO and SS can be a result of partial biohydrogenation of C18:2 and C18:3 FA and of the desaturation of C18:0 in the mammary gland (Kennelly, 1996). Most of CLA isomers levels were increased when the goat fed diet added with sunflower whole seeds or oil. Mir et al. (1999) suggest that substantial increase in CLA content in milk of small ruminants can be achieved by feeding PUFA rich oilseeds. The increased dietary intake of C18:3 with SS or SO resulted an increasing levels of C18:1 trans-11 and increased CLA C18:2 cis-9, trans-11 by Δ9-desaturase activity. There are some studies tried to increase milk content from CLA using a dietary addition with sunflower oil or sunflower whole seeds (Petit et al., 2004; Castro et al., 2009; Mohammed et al., 2011). Moreover, Omega 6 FA concentrations in milk were increased in SS or SO goats compared to Control goats. The omega 6 to omega 3 FA ratio in milk was affected by the diet, with treatments ranking from the highest to the lowest ratio as follows: Control >SS>SO.

Milk fatty acids profile of lactating Damascus goats fed basal diet with sunflower seeds whole (SS) or sunflower seeds oil (SO)


It could be concluded that feeding diets added with a daily dose of SS (50 g/h/d) or SO (20 mL/h/d) increased milk production and milk fat content. Moreover, both additives decreased total saturated fatty acid and increased total CLA and TUFA in milk with more beneficial effects for SO than SS. More studies are required for the long term beneficial effects of sunflower whole seeds and seeds oil in the diets of dairy goats.


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Table 1

Chemical composition (%) of the concentrate feed mixture (CFM1), fresh Trifolium alexandrinum, sunflower seeds whole2 or oil3 and the basal diet4 (on DM basis)

CFM Trifolium alexandrinum Sunflower whole seeds2 Basal diet4
Dry matter 91.3 13.3 91.6 52.3
Organic matter 89.9 88.2 96.5 89.1
Crude protein 14.2 14.2 21.7 14.2
Ether extract 4.1 2.6 40.3 3.35
Crude fiber 15.3 27.5 22.6 21.4
Nitrogen free-extract 56.4 43.9 11.9 50.2

The CFM consisted of 25% undecorticated cotton seed meal, 35% wheat bran, 30% corn, 3% rice bran 3% molasses, 2% limestone, 1% urea and 1% salt (NaCl).


Sunflower whole seeds contain (individual fatty acids, % of total fatty acids): C16:0, 6.4; C18:0, 5.7; C18:1, 22.6; C18:2, 65.0, C18:3, 0.3.


Sunflower oil seeds contain (individual fatty acids, % of total fatty acids): C16:0, 5.4; C18:0, 4.6; C18:1, 21.0; C18:2, 69.0.


The control diet consisted of CFM and fresh Trifolium alexandrinum at 1:1 (on DM basis); the same used in Kholif et al. (2015).

Table 2

Goats weight, feed intake, and rumen fermentation kinetics of lactating Damascus goats fed basal diet with sunflower seeds whole (SS) or sunflower seeds oil (SO)

Diets1 SEM p-value

Control SS SO
Live body weight (kg) 44.7 44.3 44.5 4.88 0.960
Dry matter intake (kg/d) 1.36 1.42 1.39 0.018 0.357
Ruminal fermentation kinetics
 pH value 6.20a 6.06a 5.80b 0.068 0.017
 Total volatile fatty acids (tVFA, mmol/L) 78.1b 90.9a 92.2a 2.32 <0.001
 Individual VFA (mol/dL)
  Acetate (C2) 58.1b 66.0a 68.5a 3.09 0.034
  Propionate (C3) 28.8 30.7 31.3 1.33 0.779
  Butyrate (C4) 10.2 12.6 14.2 0.97 0.280
  C2/C3 2.03 2.15 2.17 0.041 0.368
 Ammonia-N (mg/L) 283a 271b 269b 15.7 0.046

SEM, standard error of the mean.


Diets consisted of concentrate feed mixture and fresh Trifolium alexandrinum (1:1; Control), or Control plus 50 g SS/head/d or Control+20 mL SO/head/d.

Means at the same row with different superscript are significantly (p<0.05) different.

Table 3

Blood serum parameters of lactating Damascus goats fed basal diet with sunflower seeds whole (SS) or sunflower seeds oil (SO)

Diets1 SEM p-value

Control SS SO
Total protein (g/dL) 6.43 6.74 6.76 0.721 0.392
Albumin (g/dL) 3.44 3.61 3.67 0.494 0.587
Globulin (g/dL) 2.99 3.13 3.08 0.410 0.871
A/G ratio 1.16 1.20 1.20 0.045 0.946
Triglyceride (mg/dL) 186 182 179 23.7 0.101
Cholesterol (mg/dL) 196 190 188 24.9 0.699
Glucose (mg/dL) 62.2b 66.2a 70.3a 1.23 0.013

SEM, standard error of the mean; A/G, albumin/globulin.


Diets consisted of concentrate feed mixture and fresh Trifolium alexandrinum (1:1; Control), or Control plus 50 g SS/head/d or Control+20 mL SO/head/d.

Means at the same row with different superscript are significantly (p<0.05) different.

Table 4

Average daily milk yield and composition of lactating Damascus goats fed basal diet with sunflower seeds whole (SS) or sunflower seeds oil (SO)

Diets1 SEM p-value

Control SS SO
Milk yield (g/d) 1,140b 1,214a 1,240a 60.1 0.007
Milk composition (%)
 Fat 3.96b 4.19a 4.32a 0.050 0.002
 Protein 3.16 3.26 3.26 0.027 0.271
 Lactose 4.65b 4.72ab 4.96a 0.046 0.048
 Total solids 12.8 13.0 13.6 1.699 0.266
 Solids not fat 8.86 8.88 9.30 1.165 0.511
 Ash 0.88 0.87 0.89 0.094 0.276
 Urea N 39.2 38.4 37.6 5.32 0.121
Feed efficiency (Milk yield/DMI) 0.83b 0.85b 0.89a 0.040 0.046

SEM, standard error of the mean; DMI, dry matter intake.


Diets consisted of concentrate feed mixture and fresh Trifolium alexandrinum (1:1; Control), or Control plus 50 g SS/head/d or Control+20 mL SO/head/d.

Means at the same row with different superscripts are significantly (p<0.05) different.

Table 5

Milk fatty acids profile of lactating Damascus goats fed basal diet with sunflower seeds whole (SS) or sunflower seeds oil (SO)

Item Diets1 SEM p-value

Control SS SO
C4:0 1.60 1.50 1.20 0.172 0.185
C6:0 1.26b 1.50ab 1.64a 0.104 0.041
C8:0 2.62b 2.26b 2.84a 0.114 0.039
C10:0 6.63 5.77 5.91 0.577 0.338
C12:0 3.12 3.76 3.29 0.344 0.698
C14:0 9.93 9.28 9.77 0.925 0.762
C14:1 0.14b 0.18b 0.59a 0.017 0.000
C15:0 0.28 0.28 0.37 0.151 0.140
C16:0 28.3 27.1 25.6 3.839 0.718
C16:1 0.17b 0.21b 0.88a 0.027 0.000
C17:0 1.11a 0.39b 0.37b 0.023 0.001
C18:0 14.33 12.51 11.65 3.530 0.547
C18:1N9T 25.1 28.9 29.2 3.82 0.566
C18:1N9C 3.97ab 3.71b 4.98a 0.260 0.041
C18:2 trans-10, cis-12 0.05b 0.08a 0.09a 0.008 0.030
C18:2 cis-9, trans-11 0.10 0.12 0.13 0.020 0.664
C18:3N3 0.10b 0.13a 0.15a 0.012 0. 021
C18:3N6 0.30 0.42 0.43 0.058 0.166
C20:0 0.85 0.82 0.86 0.114 0.988
TSFA 70.0 66.1 63.5 7.26 0.193
TUFA 29.9b 33.8ab 36.4a 5.76 0.031
MUFA 29.4 33.1 35.6 5.68 0.277
PUFA 0.55 0.75 0.80 0.096 0.147
Total CLA 0.15b 0.20a 0.22a 0.017 0.007
N6/N3 3.0 3.23 2.86 0.297 0.736

SEM, standard error of the mean; TSFA, total saturated fatty acids; TUFA, total unsaturated fatty acids; MUFA, mono unsaturated fatty acids; PUFA, poly unsaturated fatty acids; CLA, conjugated linolenic acid.


Diets consisted of concentrate feed mixture and fresh Trifolium alexandrinum (1:1; Control), or Control plus 50 g SS/head/d or Control+20 mL SO/head/d.

Means at the same row with different superscripts are significantly (p<0.05) different.