Supplementary prenatal copper increases plasma triiodothyronine and brown adipose tissue uncoupling protein-1 gene expression but depresses thermogenesis in newborn lambs

Objective We tested the hypothesis that increasing dietary copper (Cu) to gravid ewes would enhance brown adipose tissue (BAT) thermogenesis in their offspring. Methods Twin-bearing ewes were assigned on d 70 of gestation to diets containing 3, 10, or 20 ppm dietary Cu (n = 8 per group). Twin lambs were assigned at birth to a cold (6°C) or warm (28°C) environmental chamber for 48 h. Blood was collected from ewes and from lambs and perirenal BAT was collected after 48 h in the environmental chambers. Results Prenatal Cu exposure increased ewe plasma triiodothyronine (T3) and thyroxine concentration (T4) (p<0.01) but prenatal Cu exposure had no effect on lamb plasma concentrations of T3, T4, glucose, or nonesterified fatty acid concentration (p≥0.08). The high level of prenatal Cu exposure depressed 48-h rectal temperature (p = 0.03). Cold exposure decreased BAT norepinephrine (NE) and increased BAT dopamine (p≤0.01), but prenatal Cu exposure had no effect on BAT cytochrome C oxidase activity or BAT NE or dopamine (p≥0.07). However, BAT of lambs from high-Cu ewes maintained higher uncoupling protein-1 (UCP1) gene expression than BAT of lambs from low- and medium-Cu ewes following warm or cold exposure in environmental chambers (p = 0.02). Cold exposure caused near depletion of BAT lipid by 48 h (p<0.001), increased BAT cytochrome c oxidase activity (p< 0.01), and depressed plasma fatty acid concentrations (p<0.001). Conclusion Although prenatal Cu exposure increased BAT UCP1 expression during warm and cold exposure, prenatal cold Cu exposure depressed 48-h rectal temperature. Cold exposure decreased BAT lipid content by over 80% and decreased lamb plasma fatty acid concentration by over 40%, indicating that fuel reserves for thermogenesis were nearly depleted by 48 h of cold exposure.


INTRODUCTION
Approximately one-half of maximal thermogenic response to cold stress in newborn lambs is derived from nonshivering thermogenesis of brown adipose tissue (BAT) [1]. The ability of BAT to generate heat is due to the presence of uncoupling protein-1 (UCP1), found only in BAT mitochondria. When stimulated by cold exposure, UCP1 acts to uncouple oxidative phosphorylation from fatty acid oxidation, thereby causing BAT mitochondria to generate heat rather than ATP. Brown adipose tissue is highly innervated by the sympathetic nervous system [2] which, during cold exposure, releases norepinephrine (NE) to activate BAT thermogenesis by stimulating fatty acid oxidation and UCP1 gene expression. Additionally, NE activates type II thyroxine 5' deiodinase in BAT to convert thyroxine (T 4 ) to the more active thyroid hormone triiodothyronine (T 3 ) [3]. Local synthesis of T 3 is also an important regwww.ajas.info 507 Smith et al (2020) Asian-Australas J Anim Sci 33:506-514 ulator of UCP1 gene expression [4]. Thus, both NE and T 3 play a critical role in regulating BAT thermogenesis in newborn lambs.
Eales et al [5] reported that newborn lambs that were hypothermic due to cold exposure had low plasma Cu concentrations (0.18 μg/mL) compared to plasma Cu concentrations in lambs that did not display hypothermia (0.63 μg/mL), which suggests that maternal Cu deficiency may impair the cold tolerance of newborn lambs. There are several Cu-dependent enzyme systems that play critical roles in regulating BAT thermogenesis, including cytochrome c oxidase (COX; electron transport system) and dopamine-β-hydroxylase (conversion of dopamine to NE in the sympathetic nervous system). Additionally, Lukaski et al [6] reported that Cu deficiency in rats reduced plasma T 3 and T 4 concentrations and reduced BAT deiodinase activity, resulting in hypothermia. Therefore, we hypothesized that increasing dietary Cu to gravid ewes would enhance BAT thermogenesis in their offspring.

Ethics statement
The experimental procedures were approved by the Texas A&M University Animal Care and Use Committee of the Office of Research Compliance.

Animals and diets
Twenty-four Rambouillet ewes were determined to be twinbearing by ultrasound during the first trimester of gestation. Ewes were housed in an open-sided barn, and provided free access to fresh water and coastal bermudagrass hay containing 0.76 mg Cu/kg DM. Ewes were adapted to a high-concentrate diet for 3 wk (Table 1, without supplemental S, Mo, or Cu) that was formulated to meet NRC requirements [7], with the exception of Cu. Thereafter, ewes were assigned on approximately d 50 of gestation to 1 of 3 diets containing 3, 10, or 20 ppm dietary Cu (n = 8 lambs per Cu concentration). Additionally, each diet contained 2 ppm (DM basis) Mo and 0.28% S to limit the absorption of dietary Cu. Accounting for the dietary concentrations of Mo and S, the net available Cu concentrations [8] were 0.12, 0.36 and 0.72 ppm (DM basis) for the low-, medium-and high-Cu diets, respectively. The medium-Cu treatment was formulated to meet the estimated net available Cu requirements for 75 kg twin-bearing ewes, which is 0.63 mg Cu/d [8]. Each prenatal Cu treatment consisted of 4 ewes/pen and 2 pens/treatment. Ewes were fed individually in an open-sided barn. Food intake and body weight were measured at 7-d intervals. Food intake was calculated as total food provided minus food remaining.
Ewes were monitored at 4-h intervals prior to expected lambing. Immediately following parturition, lambs were separated from ewes, dried of amniotic fluid, weighed, and moved to a warm chamber (25°C). Initial blood samples were obtained by venipuncutre and lambs were fed 38 mL/kg BW pooled bovine colostrum. At 2 h of age, lambs were assigned randomly within twin pair to cold (6°C) or warm (28°C) environmental chambers for 48 h. At 8, 14, and 20 h of age, lambs were fed 30 mL/kg BW colostrum. Rectal temperatures were measured at 48 h. Blood was collected obtained by jugular venipuncture in heparinized vacutainer tubes from ewes at 7 d prior to birth between 0700 and 0800 before feeding and from lambs prior to slaughter at 48 h of age, and plasma was analyzed for Cu, ceruloplasmin, T 3 , T 4 , and cortisol. Glucose and nonesterified fatty acids (NEFA) also were measured in lamb plasma. Blood samples were obtained by jugular venipuncture in heparinized vacutainer tubes between 0700 and 0800, before feeding. Equal volumes of 0.9% saline were infused to replace blood volume.

Collection of brown adipose tissue
Following the 48-h environmental-chamber period, lambs were administered an overdose of sodium pentobarbital and exsanguinated, and perirenal BAT was removed completely and weighed. A portion of the BAT was snap-frozen in liquid nitrogen and stored at -80°C.

Histological analysis
Small, unfrozen samples of BAT (~100 mg) were prepared for histological analysis. Tissue samples were sliced into 1-mm size pieces and placed into a primary fixative of 3% glutaraldehyde:0.08 M sodium cacodylate buffer (pH 7.4). One perirenal BAT sample from a low-Cu, warm-chamber lamb, and one sample from a high-Cu, cold-chamber lamb were post-fixed in 20 g/L osmium tetroxide, stained with enbloc stain (2% uranylacetate in methanol), and embedded in Epon/ Araldite [9]. The embedded samples were sectioned to approximately 70-nm thickness and photographed at 60 kV with a transmission electron microscope (Zeiss 10 C, Germany).

Preparation and analysis of RNA
Total RNA was isolated from previously snap-frozen perirenal BAT as described previously [10]. Purity and yield were determined by the ratio of absorbances at 260 and 280 nm, which exceed 1.8 for all samples. A UCP1 cDNA was generated by polymerase chain reaction. The template DNA was the bovine calf UCP1 1.4-kb cDNA (generously provided by L. Casteilla, Centre de Rechere, CNRS, France) linearized with EcoR1. The primers were 5′-CTC AGC GGG CCT AAC GAC-3′ and 5′-GTT TGT TTT TCA CCA GGG-3′. UCP1 mRNA was determined by slot blot analysis as described previously [10].

Isolation of mitochondria
Mitochondria were isolated by differential centrifugation as described by Cannon and Lindberg [11]. Fresh, unfrozen BAT samples were dissected and washed immediately in sucrose buffer (250 mmol/L sucrose, 5 mmol/L TES-HCl, pH 7.2). BAT samples were homogenized in 10 volumes (wt/vol) of sucrose buffer and centrifuged at 800×g for 10 min. The pellet was resuspended to original volume in fresh sucrose buffer. The suspension was centrifuged at 800×g for 10 min to sediment nuclei and cellular debris, and the supernate was centrifuged at 10,000×g for 10 min. The mitochondria pellet was resuspended to 0.5 mL in fresh sucrose buffer. Aliquots of homogenate and mitochondrial preparations were frozen at -80°C for subsequent determination of COX activity [12], DNA [13], protein [14], and lipid [10]. Total mitochondrial protein was determined based on mitochondrial recovery from preparations.

Plasma Cu, hormones, and nonesterified fatty acids
Plasma Cu was determined by flame atomic absorption spectrophotometry (Model S 11, Thermo Jarrell Ash, Franklin, MA, USA). Blood samples were centrifuged at 2,000×g and plasma harvested and stored at -20°C. Plasma ceruloplasmin activity was measured as described previously [15]. Plasma NEFA were measured with a commercial kit (Wako Chemicals, Brentwood, NY, USA). Concentrations of T 3 , T 4 , and cortisol were measured using commercial radioimmunoassay kits (Pantex, Santa Monica, CA, USA). Dopamine and NE were measured by high-performance liquid chromatography [16].

Source of chemicals
Unless otherwise stated, biochemicals were purchased from Sigma Chemical (St. Louis, MO, USA) and Gibco BRL (Gaithersburg, MD, USA). Radiolabeled materials were obtained from Amersham (Arlington Heights, IL, USA).

Analysis of data
The data were analyzed using the Proc Mixed model for analysis of variance to obtain main effects means, subclass means, and standard errors [17]. For ewe performance data, fixed effect was Cu treatment and random effects were ewe and pen; pen was the experimental unit. For lamb data, fixed effects were prenatal Cu treatment and chamber temperature and random effect was lamb. Lambs were housed individually in chambers and individual lamb was the experimental until. The three-way interaction of prenatal dietary Cu (low, medium, and high), chamber temperature (6°C and 25°C) and time in chamber (0 and 48 h) was not significant for rectal temperature, body weight, or any plasma dependent variables. Therefore, the data for lamb body weight, rectal temperature, and lamb plasma dependent variables (as well as BAT dependent variables) were analyzed as a 2×2 factorial with prenatal dietary Cu and chamber temperature as the main effects; the model also tested the Cu×chamber temperature two-way interactions.

Ewes
There was no difference in initial or final body weight, average daily again, or dry matter intake in ewes in response to increasing concentrations of dietary Cu (p≥0.19; Table 2). Dietary Cu treatment had no effect on plasma ceruloplasmin activity and Cu concentration in ewes (p≥0.11) ( Table 3). Plasma T 3 and T 4 concentrations were higher (p<0.01) in ewes fed the high-Cu diet than in ewes fed the low-Cu diet.

Effects of prenatal Cu and cold exposure on lambs
Chamber temperature had no effect on plasma Cu concentration or ceruloplasmin activity (p>0.25), so data for these variables were pooled between warm and cold chamber tem-  (Table 3). At birth, there were no differences among prenatal Cu treatments for Cu concentration or ceruloplasmin activity (p>0.25). Plasma ceruloplasmin activity increased with age for all prenatal Cu treatment groups (p<0.001), and ceruloplasmin activity was greater in lambs from high-Cu ewes than in lambs from low-Cu ewes (p = 0.05). Prenatal Cu exposure, chamber temperature, and the Cu× temperature interaction were not significant for lamb body weight (p>0.25) ( Table 4). Rectal temperature at birth (38.6°C ±0.31°C) was not different among prenatal Cu treatments (p >0.25; data not shown). Rectal temperature was lower (p< 0.001) in cold-exposed than in warm-exposed lambs and the high level of prenatal Cu exposure depressed rectal temperature (p = 0.03) ( Table 4).
Lamb plasma T 3 concentration tended (p = 0.08) to decrease as prenatal Cu level increased (Table 4) and plasma T 3 concentration was greater (p<0.001) in cold-exposed lambs than in warm-exposed lambs. Lamb plasma T 4 concentration was unaffected by prentatal Cu or environmental temperature (p>0.25). Lamb plasma cortisol concentration was higher (p<0.001) in cold-exposed than warm-exposed lambs. The cold-induced increase in plasma cortisol concentrations was greater in lambs born to high-Cu ewes than in lambs born to low-Cu ewes (prenatal Cu×temperature interaction p = 0.05). Cold-exposed lambs had lesser plasma NEFA concentration than warm-exposed lambs (p<0.001). Plasma glucose concentration was unaffected (p>0.25) by prenatal Cu or postnatal temperature treatments.
Postnatal cold exposure decreased (p<0.001) BAT mass and lipid concentration and increased (p<0.01) BAT protein and moisture concentration, mitochondrial protein concentration, and cytochrome c oxidase activity (   3) 5.18 b 7.59 ab 9.88 a 0.14 0.05 SEM, standard error of the mean; ADG, average daily gain; DMI, dry matter intake (individual intake). 1) Low, medium, and high Cu: 3.2, 10, and 20 ppm Cu (DM basis), respectively. 2) Chamber temperature had no effect on plasma Cu or ceruloplasmin activity (p > 0.25), so data for these variables was pooled between warm and cold chamber temperatures.
3) Plasma ceruloplasmin activity increased with age for all prenatal Cu treatment groups (p < 0.001). a,b Values within a row with common superscripts do not differ (p > 0.05). dopamine concentration. The increase in BAT dopamine concentration was significant only for lambs born to high-Cu ewes (prenatal Cu×temperature interaction p = 0.04). Relative UCP1 gene expression was higher (p<0.001) in BAT from cold-exposed lambs than in BAT from warm-exposed lambs; UCP1 gene expression was virtually undetectable in BAT from lambs held at 28°C, except for BAT from high-Cu lambs (Figure 1). UCP1 gene expression was higher in BAT of lambs from high-Cu ewes than in BAT of lambs from lowand medium-Cu ewes (p = 0.02). The increase in UCP1 gene expression due to cold exposure was not affected by prenatal Cu treatment (prenatal Cu×chamber temperature interaction p>0.25).

Lamb brown adipose tissue morphology
Transmission electron micrographs were generated only for the two temperature/Cu extremes, i.e., warm-exposed lambs from low-Cu ewes and cold-exposed lambs from high-Cu ewes. Brown adipocytes from warm-exposed lambs contained large, central lipid droplets, whereas brown adipocytes from cold-exposed lambs contained virtually no visible lipid droplets ( Figure 2). The cristae of mitochondria from cold-exposed lambs were more evident than cristae in mitochondria of warm-exposed lambs.

DISCUSSION
An early report [5] suggested that increasing maternal dietary Cu may attenuate hypothermia in lambs during cold exposure. Because several Cu-dependent enzyme systems are involved in regulating BAT thermogenesis, including COX and dopamine-β-hydroxylase, we hypothesized that increas-ing Cu intake in gravid ewes would improve cold tolerance in newborn lamb. However, opposite to our hypothesis, increased Cu intake by ewes resulted in more rapid heat loss in

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Smith et al (2020) Asian-Australas J Anim Sci 33:506-514 cold-exposed lambs, which may have been caused by increased UCP1 expression in lambs from high-Cu ewes.

Copper status
The net availabilities of Cu were estimated to be 0.12, 0.36, and 0.72 mg Cu/kg DM for the low-, medium-and high-Cu prenatal treatments, respectively [8]. Based on ewe dry matter intake, net Cu intakes for the three treatments were 0.21, 0.62, and 1.22 mg/d, respectively. Estimated net Cu requirements for 75-kg twin-bearing ewes during late gestation are 0.63 mg Cu/d [8]. Thus, dietary Cu concentration was adequate for the medium-Cu ewes, and deficient and excessive for low-and high-Cu ewes, respectively. However, the prenatal Cu treatments had no effect on plasma Cu concentration of the ewes and lambs, which would suggest that the ewes likely had adequate hepatic Cu stores prior to entry into the study.
Previous research [18] demonstrated that plasma Cu concentration and ceruloplasmin activity are low at birth and increase early postnatally, and the current study also demonstrated a postnatal increase in ceruloplasmin activity. Thus, by all measures, neither the ewes fed the low-Cu diets nor their lambs were Cu-deficient.

Thyroid hormones and brown adipose tissue thermogenesis
Rapid accumulation of lipid in fetal lamb perirenal BAT occurs from d 70 to 120, whereas increased mitochondrial biogenesis and sympathetic innervation of BAT occurs thereafter until parturition at d 150 [19]; this was confirmed in bovine BAT [20]. Relative UCP1 expression in ovine perirenal BAT reaches a peak at birth and then declines rapidly postnatally in lambs [21] and calves [20]. Giralt et al [22] examined ontogenic changes in type II thyroxine 5'-deiodinase activity in bovine BAT, which first appeared in fetal perirenal BAT at d 60 of gestation and increased rapidly until reaching a peak at d 210 of gestation. Giralt et al [22] hypothesized that endogenous production of T 3 may be involved in prenatal induction of UCP1 expression in BAT; this subsequently was confirmed [23].
Treating rats with T 3 for 5 d and then holding them at 22°C increased UCP1 expression in interscapular BAT [24]. Cold exposure increased interscapular BAT UCP1 expression, but under these conditions, T 3 did not increase UCP1 expression further. In the current study, lambs from ewes fed the lowest supplemental Cu had the highest rectal temperatures and concomitantly the highest plasma T 3 . However, UCP1 expression was highest in lambs from ewes fed the high-Cu diets and in fact, UCP1 expression in BAT from the warmexposed lambs was detectable only in BAT of calves from high-Cu ewes. Furthermore, the electron micrographs suggest loss of cristae integrity in BAT of lambs from warm-exposed ewes. Thus, circulating T 3 concentrations in the lambs may have been responsible for the elevated rectal temperatures in the low-Cu lambs, but did not appear to regulate UCP1 gene expression.

Norepinephrine and brown adipose tissue thermogenesis
One of the key changes that occurs in BAT late in gestation is the development of sympathetic innervation [25], which increases the activity of thyroxine 5′-deiodinase [26,27]. In addition to its role in acute activation of BAT thermogenesis, NE also is involved in long-term modulation of BAT growth and development during cold stress by enhancing differentiation of BAT precursor cells, mitochondrial proliferation, and UCP1 expression via β 3 -and α 1 -adrenergic receptor pathways [28]. Norepinephrine increases the activity of type II thyroxine 5′-deiodinase and thereby promotes local production T 3 , which in turn increases UCP1 expression in BAT [26].
Consistent with the higher plasma T 3 concentration in low-Cu lambs, NE concentration also were higher in BAT from the low-Cu lambs. Unexpectedly, NE concentration was higher, and dopamine concentration was lower, in BAT from warmexposed lambs than in BAT from cold-exposed lambs. This may have been in response to increased NE secretion caused by elevated sympathetic stimulation.

Cold exposure and brown adipose tissue thermogenesis
We have investigated the postnatal changes in perirenal BAT in neonatal calves, and reported that COX activity of perirenal BAT was highest at birth and decreased substantially after 7 d of warm exposure [29]. Trayhurn et al [30] reported a similar postnatal decline in COX activity in warm-exposed, neonatal goats. The results of the current study confirm earlier observations, in that COX activity was approximately threefold higher in BAT of cold-exposed lambs than in warmexposed lambs. However, COX activity was unaffected by prenatal Cu supplementation.
Especially noteworthy was the greater decline in rectal temperature in lambs from high-Cu ewes (data pooled across chamber temperatures), in spite of their elevated BAT UCP1 gene expression. Cold exposure drastically depleted BAT lipid regardless of prenatal Cu exposure, and the electron micrographs suggest that any remaining lipid in BAT after cold exposure resided primarily in membrane phospholipids. The data indicate that BAT lipid stores of all cold-exposed lambs had been depleted rapidly, leading to an inability to further generate heat via BAT nonshivering thermogenesis. Plasma NEFA concentration was lower in cold-exposed lambs, which is consistent with the depletion of BAT lipid stores seen by 48 h.
In conclusion, prenatal supplementation of Cu increased plasma T 3 concentration and BAT UCP1 expression, but this may have caused reduced tolerance to cold exposure. The results of this study suggest that the inability of lambs to maintain body temperatures during extended cold exposure is due to depletion of lipid stores in BAT. The data of the current study indicate that, if lambs are born at a time when ambient temperatures are near freezing, they have only limited ability to maintain body temperature via non-shivering, BAT thermogenesis. Conversely, lambs born in warm environments maintain their lipid stores, but rapidly lose capacity for BAT thermogenesis, as reflected by loss of UCP1 expression by 48 h of age. Prenatal Cu supplementation delays the loss of UCP1 expression, but that may exacerbate body heat loss during cold exposure. What could not be determined from the current study was the mechanism of action by which increasing maternal intake of Cu enhanced UCP1 gene expression in BAT of the newborn lambs.

CONFLICT OF INTEREST
We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.