Milk Production, Milk Composition, Live Weight Change and Milk Fatty Acid Composition in Lactating Dairy Cows in Response to Whole Linseed Supplementation

Wisitiporn Suksombat; Chayapol Meeprom; Rattakorn Mirattanaphrai

doi:10.5713/ajas.2013.13027

Suksombat, Meeprom, and Mirattanaphrai: Milk Production, Milk Composition, Live Weight Change and Milk Fatty Acid Composition in Lactating Dairy Cows in Response to Whole Linseed Supplementation

Article

Asian-Australasian Journal of Animal Sciences (AJAS) 2013; 26(8): 1111-1118.

https://doi.org/10.5713/ajas.2013.13027

Milk Production, Milk Composition, Live Weight Change and Milk Fatty Acid Composition in Lactating Dairy Cows in Response to Whole Linseed Supplementation

Wisitiporn Suksombat^*, Chayapol Meeprom, Rattakorn Mirattanaphrai

School of Animal Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, University Avenue, Amphur Muang, Nakhon Ratchasima, 30000, Thailand

^*Corresponding Author: Wisitiporn Suksombat.
Tel: +66-44-224152, Fax: +66-44-224150, E-mail: wisitpor@sut.ac.th

Received January 11, 2013 Accepted March 12, 2013 Revised March 27, 2013

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/ which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The objective of this study was to determine the effects of whole linseed supplementation on performances and milk fatty acid composition of dairy cows. Thirty six Holstein Friesian crossbred lactating dairy cows were blocked by milking days first and then stratified random balanced for milk yields and body weight into three groups of 12 cows each. The control group received 300 g of palm oil. The second group was supplemented with 344 g/d of top-dressed whole linseed plus 150 g of palm oil and the third group was supplemented with 688 g/d of top-dressed whole linseed. All cows also received ad libitum grass silage (Brachiaria ruziziensis), had free access to clean water and were individually housed in a free-stall unit and individually fed according to treatments. Residual feeds were collected on 2 consecutive days weekly and at the end of the experiment. Feed samples were pooled to make representative samples for proximate and detergent analyses. Daily milk yields were recorded. Milk samples were collected on 2 consecutive days weekly. Live weights were recorded at the start and at the end of the experiment. Milk samples were taken on d 56 of the experiment and subjected to milk fatty acid composition. The results showed no statistical significant differences in intakes, live weight change, milk yields and milk compositions, however, C18:1, C18:3 and unsaturated FAs were increased while saturated FAs were reduced by whole linseed supplementation. It is recommended that the addition of 300 g/d oil from whole linseed could be beneficial to lactating dairy cows in early lactation.

Keywords: Whole Linseed; Milk Production; Milk Composition; Milk Fatty Acids; Dairy Cows

INTRODUCTION

Linseed, also known as flaxseed, is a seed obtained from the flaxplant (Linum usitatissimum). The oil in linseed contains high levels of α-Linoleic acid (a particular form of Omega-3 fatty acid), it is used as a nutritional supplement. The α-linolenic acid (ALA) in flaxseed oil is suitable for cooking, as it can withstand temperatures up to 177°C for two hours. Regular flaxseed oil contains between 52% and 63% ALA (C18:3 n-3). Studies (Ramon et al., 2000; Brouwer et al., 2004) have shown a relationship between ALA and an increased risk of prostate cancer. This risk was found to be irrespective of source (e.g., meat, vegetable oil; De Stéfani et al., 2000). Alternatively, at least one meta analysis has found a weak protective association between dietary ALA intake and prostate cancer risk (Simon et al., 2009). High levels of polyunsaturated fatty acids (particularly eicosapentaenoic C20:5 (EPA) and docosahexaenoic C22:6 (DHA) acids) are thought to protect against cardiovascular disease. There is some evidence that α-linolenic acid (C18:3 n-3) can be elongated and desaturated by mammalian enzyme systems to produce small amounts of C20:5 and C22:6.

Many papers were published during the last 2–3 decades which describe FA profile of the milk from cows fed on different types of diets using different sources of fats and FA (Glasser et al., 2008; Moallem, 2009; Petit and Côrtes, 2010). However, despite the large amount of data published in a number of reviews, no clear conclusions have yet been drawn on the way in which the factors depending on feeding or animals influence milk fat and milk FA. Milk fat contains over 400 individual fatty acids and their isomers. Cow milk contains large amounts of SFA, particularly C14:0 and C16:0, which determine physiological dysfunctions including higher plasma cholesterol, and small amounts of MUFA, PUFA and omega-3 fatty acids with beneficial effects on human health. The problem is how to modify milk FA profile to make it suitable for human health. One solution is to feed whole oleaginous plants or oils from them to the cows. Canola oil and flaxseeds oil may influence milk FA level as follows: i) decrease SFA, ii) increase UFA, iii) increase the proportion of the conjugated linoleic acid (CLA) and of the α-linolenic acid, and iv) decrease the FA omega 6 to FA omega 3 ratio. These possible changes rely on the very high level of the canola oil in C18:1 (50 to 55%), particularly C 18:1 n-9 and of the flaxseeds oil in C18:3 (40 to 45%), particularly C 18:3 n-3.

Health-conscious consumers are interested in buying dairy products that are rich in mono- and polyunsaturated fatty acids. Dietary saturated fatty acids are perceived to be less healthy than polyunsaturated fatty acids. For example, myristic (C14:0) and palmitic (C16:0) acids have been demonstrated to have undesirable hypercholesterolemic effects and to increase the risk of coronary heart disease (Berner, 1993). On the other hand, conjugated linoleic acid and the long-chain polyunsaturated fatty acids, especially linoleic (C18:2n-6) and α-linolenic (C18:3n-3), have anticarcinogenic (Parodi, 1997) and potentially cardioprotective roles in humans (Massaro et al., 1999). As a result, there has been a great deal of interest in manipulating the fatty acid profile of milk fat to respond to consumers’ concerns.

Flaxseed contains a high oil level (40% of total seed weight) with a-linolenic acid constituting approximately 55% of oil’s total fatty acids (Mustafa et al., 2002; Petit, 2002, 2003). Research has shown several health benefits of omega-3 fatty acids (including α-linolenic acid) to humans including a decrease in the incidence of cancer, cardiovascular diseases, hypertension, and arthritis and an improvement in visual ability (Simopoulos, 1996; Wright et al., 1998). Moreover, diets rich in omega-3 fatty acids (including a-linolenic acid) reduce platelet aggregation, blood triglycerides and cholesterol levels and the occurrence of blood clots, and show both antithrombotic and anti-inflammatory effects (Nash et al., 1995; Simopoulos, 1996). Reducing concentrations of C12:0 to C16:0, and replacing them with mono- and polyunsaturated fatty acids, particularly C18:3n3, could be beneficial for consumer acceptance of milk fat. Feeding cows a supplement such as flaxseed could therefore be a natural feeding option to consider when looking at modification of milk fatty acid profile. The present study aimed to determine the effect of whole linseed supplementation to lactating dairy cows on performance and fatty acid composition in milk.

MATERIALS AND METHODS

Animals and treatments

Thirty six Holstein Friesian crossbred lactating dairy cows, averaging 32±5 d in milk, 17.0±0.8 kg of milk and 435±12 kg body weight, were blocked by milking days first and then stratified random balanced for milk yields and body weight into three groups of 12 cows each. The first group (control) received approximately 8 kg of 21% CP concentrate plus 300 g of palm oil. The second group was fed the same basal diet as the control group and supplemented with 344 g/d of top-dressed whole linseed plus 150 g of palm oil and the third group was fed the same basal diet as the control group and supplemented with 688 g/d of top-dressed whole linseed. Whole linseed contained 43.7% fat, thus the supplementation of 344 and 688 g/d of whole linseed provided 150 and 300 g/d of linseed oil, respectively. Palm oil was added to balance the energy concentration in the diets. All cows also received ad libitum grass silage (Brachiaria ruziziensis; 50 d cutting age), had free access to clean water and were individually housed in a free-stall unit and individually fed according to treatments. The experiment lasted for 10 weeks with the first 2 wks as the adjustment period, followed by 8 wks of measurement period.

Measurements, sample collection, and chemical analysis

Residual feeds were weighed for two consecutive days of each period and samples were taken and dried at 60°C for 48 h. At the end of the experimental period, feed samples were composited and subsamples were taken for further chemical analysis. Samples were ground through a 1 mm screen and subjected to proximate analysis. The crude protein content was determined by Kjeldahl analysis (AOAC, 1998). Ether extract was determined by using petroleum ether in a Soxtec System (AOAC, 1998). Neutral detergent fiber and acid detergent fiber were determined by using the method described by Van Soest et al. (1991), adapted for Fiber Analyzer. Chemical analysis was expressed on the basis of the final DM.

Cows were milked twice daily at 05.00 and 15.00 h and milk yields were recorded for each cow. Milk samples (evening and morning) were collected at each milking for two consecutive days weekly and stored at 4°C with a preservative (bronopol tablet; D&F Control System, San Ramon, CA, USA) until analyzing for fat, protein, lactose and solid-not-fat contents using a Milko-Scan S50 analyzer (Tecator, Denmark). All cows were weighed at the start and end of the experiment.

Milk fatty acid analysis

Milk samples were collected from individual cows on d 56 of the experiment. Milk samples from each period were centrifuged at 2,000×g to fat cake and extraction. Lipid extraction was that of the procedures described by Hara and Radin (1978), using a volume of 18 ml of hexane and isopropanol (3:2, vol/vol)/g of fat cake. After vortexing, a sodium sulfate solution (6.7% NaSO₄ in distilled H₂O) was added at a volume of 12 ml/g of fat cake. The hexane layer was transferred to a tube containing 1 g of NaSO₄ and the hexane layer was removed after 30 min and then stored at −20°C until methylation.

Fatty acid methyl esters (FAME) were prepared by procedure described by Ostrowska et al. (2000). The procedure briefly involved that approximately 30 mg of the extracted oil was placed into a 15 ml reaction tube fitted with a teflon-lined screw cap. 1.5 ml of 0.5 M sodium hydroxide in methanol was added. The tubes were flushed with nitrogen, capped, heated at 100°C for 5 min with occasional shaking and then cooled to room temperature. 1 ml of C17:0 internal standard (2 mg/ml in hexane) and 2 ml of 14% boron trifluoride in methanol were added and heated at 100°C for 5 min with occasional shaking. After methylation was completed, 10 ml of deionized water was added. The solution was transferred to a 40 ml centrifuge tube and 5 ml of hexane was added for FAME extraction. The solution was centrifuged at 2,000×g, at 10°C for 20 min and then the hexane layer was dried over sodium sulfate and was taken into vial for analysis by gas chromatography (GC) (Hewlett Packard GC system HP6890 A; Hewlett Packard, Avondale, PA) equipped with a 100 m×0.25 mm fused silica capillary column (SP2560, Supelco Inc, Bellefonte, PA, USA). Injector and detector temperatures were 240°C. The column temperature was kept at 70°C for 4 min, then increased at 13°C/min to 175°C and held at 175°C for 27 min, then increased at 4°C/min to 215°C and held at 215°C for 31 min.

Statistical analysis

Measurements of intake, milk production, milk composition, milk fatty acids and body weight change were analyzed by ANOVA for a randomized complete block design using the Statistical Analysis System (SAS, 1996). Differences between treatment means were statistically compared using Least Significant Differences (Steel and Torrie, 1980).

RESULTS AND DISCUSSION

Chemical composition of feeds is presented in Table 1. The crude fat content and energy values of grass silage were low. This is probably because forage was harvested at a more mature stage (50 d cutting age) and, consequently, resulting in low DM and CP degradability (46.2 and 53.1%, respectively). Fatty acid composition of feeds is given in Table 2. Grass silage was rich in C16:0 while concentrate was rich in C18:1. Palm oil was rich in C16:0 and C18:1 whereas whole linseed was rich in C18:3, C18:1 and C18:2. DM intakes of the experimental cows were similar (p>0.05) while CP and net energy for lactation (NELP) intakes of supplemented groups were significantly higher (p<0.05) than the control group (Table 3). CP intakes increased with increasing WLS supplementation and NELP intakes increased with increasing WLS/oil supplementation. Petit et al. (2005) suggested that the high-oil seed in the diet might depress the DM intake; however, addition of FA from oilseeds (approximately 30 g/kg DM) has no effect on DM intake (Allen, 2000). Martin et al. (2008) concluded that lactating dairy cows fed diet supplemented with linseed oil had significantly lower DM intake compared to the control diet, however, no negative effects were found as cows were supplemented with crude linseed or extruded linseed.

In general, whole linseed is readily accepted by dairy cows, and feeding up to 15% of the total dietary dry matter had no effect on dry matter intake of dairy cows in the early (Petit, 2002), mid (Kennelly and Khorasani, 1992; Secchiari et al., 2003), or late (Martin et al., 2008) stages of lactation. Kennelly (1996) suggested that the addition of fat to ruminant diets in the form of oilseeds would have less detrimental effects on dry matter intake due to a slower release of oil from the seed, thus resulting in no decrease in dry matter intake when feeding whole linseed. In fact, declines in dry matter intake with fat-supplemented diets appear to be related to negative effects of fats. Feeding whole (Petit et al., 2002), rolled or extruded (Doreau et al., 2009) linseed had no effect on ruminal concentrations of ammonia N, and total and individual volatile fatty acids, thus explaining the general lack of effect on dry matter intake.

Milk yields were not significantly increased by linseed supplementation in the present study (Table 4). The higher milk yields were published in some studies (Petit et al., 2001, Petit, 2002, Petit et al., 2004). Moallem (2009) reported that the average daily milk production was 1.2 kg (2.7%) higher in the dairy cows supplemented with extruded linseed at 40 g/kg DM compared to the control diet. However, this finding disagreed with previous studies where linseed was used in dairy cow diets (Petit et al., 2002; Ward et al., 2002; Gonthier et al., 2005). In those studies, the linseed was supplemented more than 100 g/kg DM in the diet. Martin et al. (2008) found that milk yields of dairy cows supplemented with linseed oil was decreased while there was no negative effect on dairy cows supplemented with crude linseed or extruded linseed. Soita et al. (2003) found no difference in milk yields of cows in the early stage of lactation fed a diet of 1.0% whole linseed and those fed no linseed. On the other hand, dairy cows in the early stage of lactation fed 9.6% of whole linseed in the dietary dry matter had a 8.1% decrease in milk yield compared with those fed a control diet with no linseed (Khorasani and Kennelly, 1994). Whole linseed intake was increased in short-term experiments (Petit et al., 2004) and milk production of cows in the early stage of lactation was decreased (Khorasani and Kennelly, 1994) although there was no difference in long-term experiments (Petit, 2002; Petit and Benchaar, 2007).

Inclusion of whole linseed in the diet of dairy cows had no effect on milk fat concentration and milk fat yield (Table 4). Feeding 10.4% of whole linseed to dairy cows in the early stage of lactation (Petit, 2002) and 1.8% of whole linseed to those in the late stage of lactation (Secchiari et al., 2003) had no effect on milk fat concentration and milk fat yield compared with cows fed a diet with no linseed. Milk fat concentration of dairy cows in the early stage of lactation fed diets of 9.7% whole linseed was similar to that of cows fed a control diet with no added fat but 4% fat-corrected milk yield and milk fat yield were higher (Petit et al., 2004). Inclusion of whole linseed in the diet of dairy cows in the mid stage of lactation at levels ranging from 5 to 15% (Kennelly and Khorasani, 1992) and at 11.1% (Petit et al., 2009) of the total dry matter had no effect on fat percentage (range 3.4 to 3.6% and 3.96 to 4.06%, respectively) and milk fat yield. Similar results were observed for dairy cows in the early stage of lactation fed diets of 1.0% (Soita et al., 2003), 10.0% (Khorasani and Kennelly, 1994), and 11.4% (Petit and Benchaar, 2007) whole linseed and cows in the late stage of lactation fed diets of 12.4% whole linseed (Martin et al., 2008) compared with cows fed a control diet with no linseed. In contrast, dairy cows in the mid stage of lactation fed diets of 5, 10 and 15% of whole flaxseed were higher milk fat concentrations than those fed a control diet with no linseed although there was no difference in milk fat yield (Petit and Gagnon, 2009).

Milk protein concentration and milk protein yield were not affected by linseed supplementation (Table 4). Feeding diets of 7 to 12.4% whole untreated linseed to dairy cows in the early stage of lactation had no effect on milk protein concentration compared with feeding a control diet with no linseed (Khorasani and Kennelly, 1994; Petit et al., 2004; Martin et al., 2008), increased by 3.8% (Petit, 2002) milk protein concentration in one study and decreased milk protein concentration by 9.1% in another study (Petit et al., 2005). Moreover, feeding diets of 9.7% whole linseed compared with calcium salts of palm oil resulted in a 5.2% increase in milk protein concentration (Petit et al., 2004). Milk protein yield increased significantly when cows in the early stage of lactation were fed a diet of 9.7 to 10.4% whole linseed as a result of higher milk yield (Petit et al., 2004) and higher milk protein concentration (Petit, 2002). Feeding diets of 5 to 15% whole linseed to cows in the mid stage of lactation had no effect on milk protein concentration and milk protein yield (Petit and Gagnon, 2009; Petit et al., 2009) and similar results were obtained when cows in the early stage of lactation were fed diets of 1% (Soita et al., 2003) and 11.4% (Petit and Benchaar, 2007) whole linseed. However, although there was no difference on milk protein yield, whole linseed inclusion in the diet of dairy cows in the mid stage of lactation at levels ranging from 5 to 15% of the total dry matter resulted in a linear decrease in milk protein concentration (3.21% for control animals compared with 3.13% for cows fed 15% linseed) with increasing level of linseed inclusion (Kennelly and Khorasani, 1992). Moreover, feeding diets of 11.8% whole linseed between weeks 20 and 30 of lactation in diets containing 16 or 18% crude protein had no effect on milk protein concentration and milk protein yield compared with feeding no linseed (Petit et al., 2005). According to Schingoethe et al. (1996), the effect of fat supplementation on milk protein depends on the source of fatty acids being fed. It showed that feeding diets of 12.5 to 12.7% linseed reduced microbial crude protein flow to the duodenum and microbial efficiencies (true and apparent) in dairy cows in the late stage of lactation, thus decreasing the amount of microbial protein supplied for milk protein synthesis (Gonthier et al., 2004). Differences in the basal diets may also explain the discrepancies among studies as different sources of forage were used and various ratios of forage to concentrate.

The present study found no effect of whole linseed supplementation on lactose content and lactose yield (Table 4). The effect of feeding linseed on milk lactose concentration was unclear. Feeding diets of whole linseed at 7 to 10% of the dry matter to dairy cows in the early stage of lactation (Khorasani and Kennelly, 1994), up to 15% to dairy cows in the mid (Kennelly and Khorasani, 1992), and late stage of lactation (Secchiari et al., 2003; Martin et al., 2008) had no effect on milk lactose concentration compared with cows fed a control diet with no linseed. In contrast, milk lactose concentration was decreased when feeding diets of 11.8% whole linseed to dairy cows in the mid stage of lactation (Petit et al., 2005) and increased compared with feeding calcium salts of palm oil although there was no difference between feeding whole linseed and micronized soybeans (Petit, 2002). In the present study, C18:1, C18:3 and Unsaturated FAs were increased whereas saturated FAs were decreased by whole linseed supplementation (Table 5). According to Kennelly (1996), the extent to which oilseed feeding altered the fatty acid composition of milk depended on a number of factors including: i) the degree of ruminal biohydrogenation; ii) composition of the non-lipid component of the diet; iii) influence of the lipid source on microbial fatty acid synthesis and de novo synthesis of fatty acids in the mammary gland; iv) stage of lactation; and v) intestinal and mammary gland desaturase activity. In contrast, milk from cows fed no linseed product contained usually less than 1% α-linolenic acid (Glasser et al., 2008). These data suggest that the ability of the mammary gland to secrete α-linolenic acid in milk is not a limiting factor in feeding strategies designed to alter milk composition, but that protection against biohydrogenation by rumen microbes is the critical point for the transfer of α-linolenic acid from the diet to milk. In general, milk fatty acid profile was slightly improved for better human health by feeding linseed, as shown by higher concentrations of fatty acids (e.g., omega 3) recognized as being beneficial to reduce the incidence of cancer, cardiovascular diseases, hypertension, arthritis and an improvement of visual acuity (Simopoulos, 1996; Wright et al., 1998). Recently, an excellent review has been published which provides quantitative estimates of the impact of linseed and other oilseed supplements on milk fatty acid profile (Glasser et al., 2008). However, concentrations of polyunsaturated fatty acids in milk of cows fed linseed usually did not exceed 3 to 4% of total fatty acids (Kennelly, 1996). The extent of change in the concentration of fatty acids in milk was proportional to the level of inclusion of linseed in the diet (Kennelly, 1996; Petit and Gagnon, 2009). Percentages of trans-18:1 and total conjugated linolenic acid in milk fat was increased linearly as the amount of linseed in the diet was increased (Glasser et al., 2008). Diets with linseed oil given as extruded, micronized, or ground seeds decreased milk fat concentrations of C6 to C8, C10 to C14, and 16:0, and increased those of total C18, trans-18:1, conjugated linoleic acid, and linolenic acid more than whole seeds (Glasser et al., 2008).

Linseed is a potential alternative to fish oil as dietary source of unsaturated fatty acids. Dairy cows offered whole linseed at 72 g/kg DM had higher milk cis-9 C18:1, C18:3n-3, MUFA and PUFA than those cows fed the control diet (Petit and Côrtes, 2010). Similar results were also found as the dairy cows fed the basal diet supplemented with extruded linseed at 28 g/kg DM (Moallem, 2009). Fuentes et al. (2008) reported that dairy cows supplemented with 5.5% DM extruded linseed for 90 d had greater C18:1 cis9, CLA, Ω3, MUFA and PUFA compared to the control diet.

CONCLUSIONS

The present study clearly indicated that supplementation of whole linseed to lactating dairy cows had no effect on milk yield and milk composition, however, C18:1, C18:3 and unsaturated fatty acids were increased and saturated fatty acids were decreased by WLS supplementation. Therefore, 300 g/d of oil from whole linseed should be supplemented to lactating dairy cows’ diets.

ACKNOWLEDGMENTS

Authors would like to express special thanks to the Dairy Farming Promotion Organization of Thailand (DPO), the Center for Scientific and Technological Equipment for their great support. Financial support was provided by the Institute of Research and Development, Suranaree University of Technology.

The work was supported by the Institute of Research and Development, Suranaree University of Technology.

Table 1

Chemical composition of concentrate and grass silage used in the experiment

% Dry matter	Concentrate	Grass silage	Palm oil	Whole linseed
Dry matter	92.13	26.37	-	91.70
Crude protein	21.24	7.52	-	21.34
Crude fat	3.92	2.13	99.00	43.70
Ash	8.56	10.08	-	3.30
Crude fiber	12.31	29.16	-	6.21
Non fiber carbohydrate	35.03	28.18	-	3.15
Neutral detergent fiber	37.69	53.28	-	36.20
Acid detergent fiber	15.64	35.37	-	22.30
Acid detergent lignin	3.92	4.73	-	4.27
NDIN	1.03	0.19	-	1.23
ADIN	0.42	0.16	-	0.48
TDN_1X (%)¹	65.80	56.50	178.20	71.98
DE_P (Mcal/kg)²	3.04	2.57	5.06	5.11
ME_P (Mcal/kg)³	2.63	2.14	5.06	4.71
NE_LP (Mcal/kg)⁴	1.66	1.31	4.05	3.12
dgDM⁵	65.80	46.20	-	-
dgCP⁵	67.50	53.10	-	-

NDIN = Neutral detergent insoluble nitrogen; ADIN = Acid detergent insoluble nitrogen; dgDM = Effective degradability of dry matter; dgCP = Effective degradability of crude protein; _1X = at maintenance level; _P = at production level; NE_LP = Net energy for lactation at production level (NRC, 2001).

¹ TDN_1X (%) = tdNFC+tdCP+(tdFA×25.25)+tdNDF-7)

² DE_P (Mcal/kg) = (((TDN_1X −((0.18×TDN_1X) −10.3))×Intake)/TDN_1X)×DE_1X

³ ME_P (Mcal/kg) = (1.01×(DE_P)−0.45)+(0.0046×(EE−3))

⁴ NE_LP (Mcal/kg) = (0.703×ME_P)−0.19, (EE>3%)

⁴ NE_LP (Mcal/kg) = (0.703×ME_P)−0.19)+((0.097×ME_P)/97)×(EE−30), (EE>3%)

⁵ Obtained from nylon bag technique (Ørskov and McDonald, 1981).

Ingredient composition of concentrate: 32% cassava distillers dried meal, 20% soybean meal, 17.5% corn distillers dried grains with solubles, 10% rice bran, 10% wheat bran, 8% molasses, 2.5% mineral and vitamin mix.

Mineral and vitamin mix: provided per kg of concentrate: vitamin A, 5,000 IU; vitamin D₃, 2,200 IU; vitamin E, 15 IU; Ca 8.5 g; P 6 g; K 9.5 g; Mg 2.4 g; Na 2.1 g; Cl 3.4 g; S 3.2 g; Co 0.16 mg; Cu 100 mg; I 1.3 mg; Mn 64 mg; Zn 64 mg; Fe 64 mg; Se 0.45 mg.

Table 2

Fatty acid composition of feeds (% of total fatty acids)

Fatty acid profile	Concentrate	Palm oil	WLS	Grass silage
C12:0	32.86	0.23	0.00	4.29
C14:0	13.37	0.51	0.04	2.87
C16:0	12.28	43.44	3.53	28.23
C18:0	2.67	4.66	7.23	15.34
C18:1	18.59	41.91	20.59	14.62
C18:2	11.27	8.63	18.47	12.28
C18:3	0.00	0.32	49.62	2.27
Others	8.96	0.30	0.52	20.10

WLS = Whole linseed.

Table 3

Effects of whole linseed supplementation on DM, CP and NE_LP intakes of dairy cows

Intake	Control (300 g PO/d)	344 g WLS+150 g PO/d	688 g WLS/d	SEM	p-value
DM (kg)
Concentrate	7.37	7.37	7.37
Grass silage	6.24	6.43	6.14	0.23	0.54
PO/WLS	0.30	0.49	0.69
Total	13.91	14.29	14.20	0.25	0.52
CP (g/d)
Concentrate	1,565	1,565	1,565
Grass silage	469	484	462	15.28	0.56
PO/WLS	0	73	147
Total	2,035^c	2,122^b	2174^a	15.58	0.03
NE_LP (Mcal/d)
Concentrate	12.23	12.23	12.23
Grass silage	8.17	8.42	8.04	0.32	0.56
PO/WLS	1.22	1.68	2.15
Total	21.62^b	22.33^a	22.43^a	0.30	0.045

PO = Palm oil; WLS = Whole linseed; SEM = Standard error of the mean; NE_LP = Net energy for lactation at production level.

Table 4

Effects of whole linseed supplementation on milk yield, milk composition, final liveweight and live weight change

Yields	Control (300 g PO/d)	344 g WLS+150 g PO/d	688 g WLS/d	SEM	p-value
3.5% FCM (kg/d)	18.3	18.8	19.7	1.01	0.265
% Fat	3.67	3.79	3.87	0.25	0.680
% Protein	2.91	2.97	3.02	0.17	0.584
% Lactose	4.98	4.86	4.91	0.32	0.926
% Solid-not-fat	8.98	8.64	8.50	0.38	0.854
% Total solid	12.65	12.43	12.37	0.46	0.893
Fat yield (g/d)	653	682	720	56	0.203
Protein yield (g/d)	518	535	562	49	0.981
Lactose yield (g/d)	886	875	913	73	0.742
Solid-not-fat yield (g/d)	1,598	1555	1581	106	0.738
Total solid yield (g/d)	2,252	2237	2301	131	0.715
Initial live weight (kg)	432	431	435	12.3	0.638
Final live weight (kg)	446	441	457	13.6	0.827
LW change (g/d)	+250	+179	+393	106	0.835

PO = Palm oil; WLS = Whole linseed; SEM = Standard error of the mean; FCM = Fat-corrected-milk.

Table 5

Effects of WLS supplementation on fatty acid composition in milk (% of total fatty acid)

Fatty acids	Control (300 g PO/d)	344 g WLS+150 g PO/d	688 g WLS/d	SEM	p-value
C4:0	4.41	4.56	4.47	0.602	0.593
C6:0	2.84	2.73	2.44	0.396	0.525
C8:0	1.78	1.63	1.42	0.294	0.737
C10:0	4.33	3.71	3.32	0.707	0.654
C12:0	4.65	3.81	3.33	0.644	0.456
C14:0	12.40	11.50	10.60	1.120	0.895
C14:1	1.21	1.18	1.31	0.261	0.413
C16:0	32.70	29.40	26.80	2.220	0.693
C16:1	1.65	1.54	1.69	0.376	0.351
C18:0	7.80	8.91	9.00	1.390	0.323
C18:1	18.13^c	22.08^b	25.25^a	0.382	0.034
C18:2	1.73	1.87	1.91	0.246	0.613
C18:3	0.23^b	0.44^ab	0.67^a	0.112	0.048
C20:0	0.07	0.07	0.13	0.069	0.277
C20:1	0.03	0.03	0.03	0.010	0.594
Others	6.04	6.53	7.63	1.023	0.342
Saturated FA	70.98^a	66.32^b	61.51^c	0.654	0.036
Unsaturated FA	22.98^c	27.14^b	30.86^a	0.542	0.037

PO = Palm oil; WLS = Whole linseed; SEM = Standard error of the mean.

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