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Mitochondrial morphology and energy metabolism in reprogrammed porcine expanded potential stem cells |
Yun Ju Lee1,2 
, Jae Hoon Song1
, Je Woo Lee1
, Tae Kyung Hong1
, Sang Jun Uhm3
, Kwonho Hong1
, Jeong-Tae Do1,*
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1Department of Stem Cell and Regenerative Biotechnology, Konkuk Institute of Technology, Konkuk University, Seoul 05029, Korea
2Biotechnology Research Institute, MGENSolutions Co., Ltd., Seoul 06591, Korea
3Department of Animal Science, Sangji University, Wonju 26339, Korea |
Correspondence:
Jeong-Tae Do, Tel: +82-24503673, Fax: +82-24551044, Email: dojt@konkuk.ac.kr
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Received: 22 July 2024 • Revised: 17 August 2024 • Accepted: 26 August 2024 |
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| Abstract |
Objective
Expanded potential stem cells (EPSCs) are stem cells that can differentiate into embryonic and extraembryonic lineages, including extraembryonic endoderm and trophoblast lineages. Therefore, EPSCs have great potential in advancing regenerative medicine, elucidating disease mechanisms, and exploring early embryonic development. However, the generation and characterization of EPSCs in pigs have not been thoroughly explored. In this study, we successfully generated porcine EPSCs (pEPSCs).
Methods
We reprogrammed porcine fetal fibroblasts (PFFs) using an integration-free method with Sendai virus vectors.
Results
The resulting pEPSCs expressed key pluripotency markers and demonstrated the ability to differentiate between embryonic and extraembryonic lineages. Notably, reprogramming into pEPSCs was associated with a transformation of mitochondrial morphology from the elongated form observed in PFFs to a globular shape, reflecting potential alterations in energy metabolism. We observed significant remodeling of mitochondrial morphology and a subsequent shift towards glycolytic energy dependence during the reprogramming of PFFs into pEPSCs.
Conclusion
Our findings provide valuable insights into the characteristics of EPSCs in pigs and highlight their potential applications in regenerative medicine, disease modeling, and emerging fields such as cell-based meat production.
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| Keywords:
Cellular Metabolism; Cellular Reprogramming; Extended Pluripotent Stem Cells (EPSCs); Mitochondrial Dynamics; Pig Cells |
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