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Dietary Mulberry leaf 1-deoxynijirimycin supplementation shortens villus height and improves intestinal barrier in fattening rabbits |
Shaocong Li1 
, Tao Li1, Zijie Jiang1
, Wenyu Hou1
, Qirui Hou1
, Boris Ramos Serrano2
, Adileidys Ruiz Barcenas2
, Weiguo Zhao1,*
, Yuhua Wang1
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1Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China
2Plant Protein and Bionatural Products Research Center, Havana, Cuba |
Correspondence:
Weiguo Zhao, Tel: +86+13775327390, Fax: none, Email: wgzsri@126.com
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Received: 23 February 2024 • Revised: 4 April 2024 • Accepted: 20 May 2024 |
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| Abstract |
Objective
The current study investigated the effects of mulberry 1-deoxynijirimycin (DNJ) on the digestion ability, intestinal morphology, and intestinal barrier of rabbits.
Methods
A total of 36 New Zealand White rabbits (male) about 45 days old (mean body weight of 1.05 0.04 kg) were reared and commercial diets were employed, and afterwards divided into three groups (n= 12) with different levels of DNJ extract additive in feed: T0 (0 g/kg), T1 (0.35 g/kg), T2 (0.7 g/kg) for 28 d.
Results
The results demonstrated that T2 decreased the average daily gain (ADG) (P < 0.05). T1 and T2 decreased villus height and inflammatory factor levels as compared with T0 (P < 0.05). DNJ significantly decreased the content of valeric acid (P < 0.05). The content of acetic acid, propionic acid, iso butyric acid, iso valeric acid in T1 were higher than those in T0 and T2 (P < 0.05). The content of butyric acid in T2 was lower than it in T0 and T1 (P < 0.05). The content of caproic acid was firstly improved then reduced as the DNJ concentration improved (P < 0.05). T2 significantly increased the abundance of dgA-11_gut_group and Christensenellaceae_R-7_group while decreased Bacteroide and Ralstonia as compared with T0 (P < 0.05). Compared with T0, T1 and T2 significantly improved the gene expression of JAM2, JAM3, mucin4, mucin6 (P < 0.05), T1 significantly decreased the expression of occluding while T2 significantly increased (P < 0.05), T2 significantly increased the expression of claudin1 and claudin2 (P < 0.05).
Conclusion
DNJ at high level changed microbiome compositions, inhibited inflammation, and improved intestinal barrier while it decreased the growth performance and shorted villus height in rabbit jejunum by regulating short chain fatty acid (SCFA) compositions in rabbits.
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| Keywords:
DNJ; Digestion Ability; Gut Health; Microbiome; Mulberry Leaves; Rabbits |
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