The variation of IGF2 maker is associated with growth traits in Thai native (Kradon) pigs

Ampaporn, Kessara; Suwannasing, Rattikan; Phongphanich, Pitchayanipa; Tunim, Supanon; Duangjinda, Monchai

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Anim Biosci > Accepted Articles

Article

https://doi.org/10.5713/ab.22.0431 [Accepted] Published online May 4, 2023.

The variation of IGF2 maker is associated with growth traits in Thai native (Kradon) pigs

Kessara Ampaporn^1,*

, Rattikan Suwannasing²

, Pitchayanipa Phongphanich²

, Supanon Tunim²

, Monchai Duangjinda³

¹Department of Animal Science, Faculty of Natural Resources, Rajamangala University of Technology Isan Sakon Nakon Campus, Sakon Nakon 47160, Thailand
²Animal Production and Management Division, Faculty of Natural Resources, Prince of Songkla University, Songkla 90112, Thailand
³Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand

Correspondence: Kessara Ampaporn, Tel: +66-42771440, Fax: +66-42771460, Email: kessara.am@rmuti.ac.th

Received: 11 November 2022 • Revised: 14 February 2023 • Accepted: 1 May 2023

Abstract

Objective
To investigate polymorphisms of the MC4R and IGF2 genes and to evaluate the growth traits affected by such polymorphisms in Thai native (Kradon) pigs.
Methods
Blood samples and productive data from 91 Kradon pigs were collected. DNA was extracted and quantified, the IGF2 and MC4R genes were amplified, and the polymerase chain reaction (PCR) produces were digested using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique. Genotyping was performed, and the association between genotypes and growth traits on the birth and weaning weights were evaluated.
Results
The IGF2 intron7 g.162G>C variations in Kradon pigs were found in three genotypes: (1) GG, (2) GC, and (3) CC. The GG genotype frequency was the highest followed by the GC and CC genotypes. The frequencies of the G and C alleles were 0.703 and 0.297, respectively. The MC4R genotype was found in only one genotype (GG). The IGF2 gene pattern was not associated with birth weight traits, whereas the IGF2 gene pattern was related to the weaning weight trait in Kradon pigs. Pigs with the CC and GC genotypes had higher weaning weights than ones with the GG genotype (p < 0.001).
Conclusion
Thai native Kradon pigs with the CC and GC genotypes of the IGF2 gene have higher weaning weights than pigs with the GG genotype.

Keywords: IGF2 Gene; Kradon Pig; M4CR Gene; Thai Native Pig