Green Cabbage Supplementation Influences the Gene Expression and Fatty Acid Levels of Adipose Tissue in Chinese Wanxi White Geese

Wang, Bin; Liu, Zhengquan; Chen, Xingyong; Zhang, Cheng; Geng, Zhaoyu

doi:.0.0.0

Article

https://doi.org/10.5713/ab.22.0345 [Accepted] Published online May 2, 2023.

Green Cabbage Supplementation Influences the Gene Expression and Fatty Acid Levels of Adipose Tissue in Chinese Wanxi White Geese

Bin Wang¹

¹Tongcheng Teachers College, Tongcheng 231400, China
²College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Correspondence: Xingyong Chen, Tel: +86-551-65785519, Fax: +86-551-65785519, Email: chenxingyong@ahau.edu.cn

Received: 8 September 2022 • Revised: 26 January 2023 • Accepted: 5 March 2023

Abstract

Objective
Dietary green cabbage was evaluated for its impact on fatty acid synthetic ability in different adipose tissues during fattening of Wanxi white geese.
Methods
A total of 256 Wanxi white geese at their70 days were randomly allocated into 4 groups with 4 replicates and fed 0, 15%, 30%, and 45% fresh green cabbage (relative to dry matter), respectively, in each group. Adipose tissues (subcutaneous and abdominal fat), liver and blood were collected from 4 birds in each replicate at their 70, 80, 90, and 100 days for fatty acid composition, relative gene expression and serum lipid analysis. Two-way or three-way ANOVA was used for analysis.
Results
The contents of palmitic acid (C16:0), palmitoleic acid (C16:1), linoleic acid (C18:2), and alpha-linolenic acid (C18:3) were feeding time dependently increased. The C16:0 and stearic acid (C18:0) were higher in abdominal fat, while C16:1, oleic acid (C18:1), and C18:2 were higher in subcutaneous fat. Geese fed 45% green cabbage exhibited highest level of C18:3. Geese fed green cabbage for 30 d exhibited higher level of C16:0 and C18:0 in abdominal fat, while geese fed 30-45% green cabbage exhibited higher C18:3 in subcutaneous fat. The expression of Acsl1 (P = 0.003) and Scd1(P<0.0001) were decreased with green cabbage addition. Interaction between feeding time and adipose tissue affected Elovl6, Acsl1 and Scd1 gene expression levels (P = 0.013, P = 0.003, P = 0.005). Feeding time only affected serum lipid levels of FFA and CM. Higher contents of C16:0, C18:1 and C18:3 were associated with greater mRNA expression of Scd1 (P<0.0001), while higher level of C18:2 was associated with less mRNA expression of Scd1 (P<0.0001).
Conclusion
Considering content of C18:2 and C18:3, 30% addition of green cabbage could be considered for fattening for 30 days in Wanxi white geese.

Keywords: Fattening; Fatty Acid; Green Cabbage; Stearoyl-CoA Desaturase; Wanxi White Goose