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Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.22.0266    [Accepted] Published online January 11, 2023.
Expression of heat shock protein genes in Simmental cattle exposed to heat stress
Luis Felipe Guzmán1  , Guillermo Martínez-Velázquez2,*  , Fernando Villaseñor-González3  , Vicente Eliezer Vega-Murillo4  , José Antonio Palacios-Fránquez2, Ángel Ríos-Utrera5  , Moisés Montaño-Bermúdez6,* 
1National Genetic Resources Center, National Institute of Forestry, Agricultural and Livestock Researches, Tepatitlán, Jalisco, Postal Code 47600, Mexico
2C.E. Santiago Ixcuintla, National Institute of Forestry, Agricultural and Livestock Researches, Santiago Ixcuintla, Nayarit, Postal Code 63600, Mexico
3C.E. Centro Altos de Jalisco, National Institute of Forestry, Agricultural and Livestock Researches, Tepatitlán, Jalisco, Postal Code 47600, Mexico
4Faculty of Veterinary Medicine and Zootechnics, Universidad Veracruzana, Veracruz, Veracruz, Postal Code 91710, Mexico
5C.E. La Posta, National Institute of Forestry, Agricultural and Livestock Researches, Medellín, Veracruz, Postal Code 94277, Mexico
6National Disciplinary Research Center for Physiology and Animal Improvement, National Institute of Forestry, Agricultural and Livestock Researches, Ajuchitán, Queretaro, Postal Code 76280, Mexico
Correspondence:  Guillermo Martínez-Velázquez, Tel: +52-55-38-71-8700 ext. 84411, Email: martinez.guillermo@inifap.gob.mx
Moisés Montaño-Bermúdez, Tel: +52-55-38-71-8700 ext. 84411, Email: martinez.guillermo@inifap.gob.mx
Received: 8 July 2022   • Revised: 8 September 2022   • Accepted: 8 July 2022
Abstract
Objective
In tropical, subtropical and arid zones, heat stress is the main cause of productivity reduction in cattle. When climate stressors occur, animals become thermal adapted through differential expression of some genes, including HSP family. The aim of this study was to determine levels of expression of HSP60, HSP70 and HSP90 genes in Simmental cattle raised in tropical environments of Mexico.
Methods
In this study, expression of HSP60, HSP70 and HSP90 genes was analyzed in 116 Simmental cattle from three farms with tropical climate located in western Mexico. Animals were sampled twice a day, in the morning and noon. Gene expression was evaluated by qPCR using probes marked with fluorescence. The MIXED procedure of SAS with repeated measures was used for all statistical analysis.
Results
HSP60 gene expression differences were found for sex (p=0.0349). HSP70 gene differences were detected for sampling hour (p=0.0042), farm (p<0.0001), sex (p=0.0476) and the interaction sampling hour x farm (p=0.0002). Gene expression differences for HSP90 were observed for farm (p<0.0001) and year (p=0.0521). HSP70 gene showed to be a better marker of heat stress than HSP60 and HSP90 genes.
Conclusion
Expression of HSP70 gene in Simmental herds of the tropical region of western México was different during early morning and noon, but the expression of the HSP60 and HSP90 genes was similar. Identification of resilient animals to heat stress will be useful in the genetic improvement of the Simmental breed.
Keywords: Gene Expression; Heat Stress; HSP Family; Simmental Cattle


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