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https://doi.org/10.5713/ab.22.0301    [Accepted] Published online November 14, 2022.
Effects of lactic acid bacteria (LAB) fermented feed and three types of LAB (L. plantarum, L. acidophilus, B. animalis) on intestinal microbiota and T cell polarization (Th1, Th2, Th17, Treg) in the intestinal lymph nodes and spleens of rats
Da Yoon Yu1  , Sang Hyon Oh1  , In Sung Kim1  , Gwang Il Kim1  , Jeong A Kim1  , Yang Soo Moon2  , Jae Cheol Jang1  , Sang Suk Lee3  , Jong Hyun Jung4  , Hwa Chun Park5  , Kwang Keun Cho1,* 
1Devision of Animal Science, Gyeongsang National University, Jinju 52725, Korea
2Division of Animal Bioscience & Integrated Biotechnology, Gyeongsang National University, Jinju 52725, Korea
3Department of Animal Science and Technology, Sunchon National University, Sunchon 57922, Korea
4Jung P&C Institute, Yongin 16950, Korea
5Dasan Genetics, Namwon 55716, Korea
Correspondence:  Kwang Keun Cho, Tel: +82-55-772-3286, Fax: +82-55-772-3689, Email: chotwo2@gnu.ac.kr
Received: 5 August 2022   • Revised: 30 August 2022   • Accepted: 16 September 2022
Abstract
Objective
In this study, we investigated the effects of Rubus coreanus-derived LAB fermented feed (RC-LAB fermented feed) and three types of LAB (Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium animalis) on the expression of transcription factors and cytokines in Th1, Th2, Th17 and Treg cells in the intestinal lymph nodes and spleens of rats. In addition, the effect on intestinal microbiota composition and body weight was investigated.
Methods
Five-week-old male rats were assigned to five treatments and eight replicates. The expression of transcription factors and cytokines of Th1, Th2, Th17, and Treg cells in the intestinal lymph nodes and spleens was analyzed using real-time reverse transcriptase polymerase chain reaction (RT-PCR) assays. Intestinal tract microbiota compositions were analyzed by next-generation sequencing (NGS; Illumina MiSeq) and quantitative polymerase chain reaction (qPCR) assays.
Results
RC-LAB fermented feed and three types of LAB increased the expression of transcription factors and cytokines in Th1, Treg cells and Galectin-9, but decreased in Th2 and Th17 cells. In addition, the intestinal microbiota composition changed, the body weight and Firmicutes to Bacteroidetes (F/B) ratio decreased, and the relative abundance of LAB increased.
Conclusion
LAB fermented feed and three types of LAB showed an immune modulation effect by inducing T cell polarization and increased lactic acid bacteria in the intestinal microbiota.
Keywords: Body Weight; Intestinal Microbiota; Lactic Acid Bacteria; Rubus coreanus; T Cell Polarization
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