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Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.22.0046    [Accepted] Published online June 24, 2022.
Effects of intracerebroventricular injection of corticotrophin releasing factor on the gene expression of ghrelin and corticotrophin releasing factor receptors in broiler chickens
Yuanli Cai1  , Zhigang Song2,* 
1College of Life Science, Qilu Normal University, Jinan, Shandong 250200, China
2Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China
Correspondence:  Zhigang Song, Tel: +86-13793808691, Fax: +86-13793808691, Email: naposong@qq.com
Received: 29 January 2022   • Revised: 28 April 2022   • Accepted: 18 May 2022
This study aimed to investigate the effects of corticotropin-releasing factor (CRF) on the feed intake of broiler chickens and explore its influencing mechanism.
The study included two trials. In trial 1, 32 male broiler chickens (Arbor Acres, Gallus gallus domesticus) were ventricle buried tubes, and they were allowed to recover for 3 days. At 8:00 AM, intracerebroventricular (ICV) injection with CRF or normal saline was performed in 10-day-old broiler chickens, which were divided into the 5, 10, and 20 µg and control (normal saline) groups according to the dose of CRF injection. In trial 2, chickens were divided into the 10 µg and control group (physiological saline) to repeat trial 1.
Results of trial 1 showed that the cumulative amount of feed intake in the 10 or 20 µg groups was considerably lower than that of the control group after ICV injection with CRF. The lowest amount of feed intake was obtained with the addition of 10 µg of CRF. In trial 2, the expression of ghrelin in the hypothalamus injected with 10 µg of CRF increased significantly, but the expression of ghrelin in various sections of the small intestine considerably decreased. The expression of CRF receptor subtypes 1 (CRFR1) in the hypothalamus and some parts of the small intestine remarkably increased, and the expression of CRF receptor subtypes 2 (CRFR2) increased only in the duodenum, whereas the expression of growth hormone secretagogue receptor (GHSR-1α) in the jejunum and ileum increased considerably after ICV injection of 10 µg of CRF.


The CRF at 10 µg increased ghrelin expression in the hypothalamus and CRFR1 expression in the small intestine, and this phenomenon was related to the suppressed feed intake of broiler chickens.
Keywords: Broiler chickens; Ghrelin; CRF; Feed Intake
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