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Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.22.0058    [Accepted] Published online May 2, 2022.
Age quadratically affects intestinal calcium and phosphorus transporter gene expression in broiler chickens
Xianliang Lv1,2  , Junfang Hao2  , Lihua Wu1,2  , Mengyuan Liu1,2  , Lei He2,3  , Yingying Qiao4  , Yanyan Cui2  , Guan Wang2  , Chunmei Zhang2  , Hongxia Qu2  , Jincheng Han2,* 
1College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
2Department of Animal Science, College of Biology and Food, Shangqiu Normal University, Shangqiu 476000, China
3College of Life Sciences, Henan Normal University, Xinxiang 453007, China
4Faculty of Biology and Technology, Sumy National Agrarian University, Sumy 19500, Ukraine
Correspondence:  Jincheng Han, Tel: +86-0370-3115-990, Fax: +86-0370-3115-990, Email: j.c.han@hotmail.com
Received: 16 February 2022   • Revised: 3 April 2022   • Accepted: 28 April 2022
Abstract
Objective
This research aimed to evaluate the effects of age on growth, tibia development, and intestinal calcium (Ca) and phosphorus (P) transporter gene expressions in broiler chickens.
Methods
A total of 224 male Arbor Acres broilers were fed with nutrient-adequate diets and reared in eight cages (28 broilers per cage). Eight broilers (one broiler per cage) were selected and killed at 5, 10, 15, 20, 25, 30, 35, and 40 days of age, respectively.
Results
Body weight continuously increased with age of broiler chickens from 5 to 40 days. The bone weight, ash weight, diameter, and length of the tibia also increased with broiler age. By contrast, the tibia ash, Ca, and P percentages quadratically changed with age (p<0.001), and the highest values of mineral contents were observed at 20, 25, and 25 days of age, respectively. The mRNA abundances of calcium-binding protein 28-kDa (CaBP-D28k), sodium-calcium exchanger 1 (NCX1), and plasma membrane ATPase 1b (PMCA1b) increased from 5 to 25 days and then decreased up to 40 days. Similar results were noted in the mRNA abundances of IIb sodium-phosphate cotransporter (NaPi-IIb), inorganic phosphate transporter 1 (PiT-1), inorganic phosphate transporter 2 (PiT-2), nuclear vitamin D receptor (nVDR), and membrane vitamin D receptor (mVDR). The mRNA abundances of Ca and P transporters and VDRs were the highest at 25 days of age.

Conclusion

These data indicate that age quadratically affects intestinal Ca and P transporter gene expression and mineral absorption capacity in broiler chickens.
Keywords: Age; Broiler Chicken; CaBP-D28k; PMCA1b; NaPi-IIb; PiT-1


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