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Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.21.0300    [Accepted] Published online January 5, 2022.
The efficiency of different types of extenders for semen cooling in stallions
Zuzana Rečková1  , Radek Filipčík1  , Katarína Soušková1  , Tomáš Kopec1,*  , Martin Hošek1  , Vojtěch Pešan1 
Mendel University in Brno (FA). Zemědělská 1, 613 00 Brno, Czech Republic
Correspondence:  Tomáš Kopec, Tel: +420-725-150-490, Fax: +420-545-133-213, Email: tomas.kopec@mendelu.cz
Received: 28 June 2021   • Revised: 3 August 2021   • Accepted: 7 December 2021
This study was focused on the influence of the skimmed milk-based extender (SM), the INRA 96 extender and BotuSemen Gold extender on parameters of stallions’ ejaculate during storage.
In this study, 14 stallions between 4 and 20 years of age were monitored. Total and progressive motility, viability and morphology of sperm were evaluated at time intervals of 24, 48 and 72 hours after collection.
The total motility, progressive motility, and values of sperm with normal morphology were significantly higher in the INRA 96 and BotuSemen Gold extenders than in the skimmed milk-based extender (p < 0.01). The sperm viability differed significantly in all extenders (p < 0.01). The highest value of sperm viability was in INRA 96 (64.69 ± 0.67%) and lowest in skimmed milk-based extender (59.70 ± 0.81%). The highest differences occurred at 72 hours of storage. Values of total motility, progressive motility and sperm viability decreased over time (p < 0.01). In case of sperm morphology there was no statistically significant decrease between 48- and 72-hour time intervals.


It can be concluded that the extenders with a chemically defined composition have shown better indicators of insemination capabilities in ejaculates than the skimmed milk-based extender. The BotuSemen Gold extender is a suitable alternative to the INRA 96, when used within 48 hours; after 72 hours of storage, however, the INRA 96 showed a higher share of viable spermatozoa.
Keywords: Fertilizing Capacity; Semen Cooling; Semen Extender; Stallion

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