Go to Top Go to Bottom
Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.21.0265    [Accepted] Published online January 5, 2022.
Mitochondrial sequence based characterization and morphometric assessment of Diara buffalo population
Karan Veer Singh1,*  , Hitesh Purohit2  , Ramesh Kumar Singh2 
1National Bureau of Animal Genetic Resources, Karnal, Haryana 132001 India
2Department of Animal Genetics and Breeding, Bihar Veterinary College, Patna, Bihar, India
Correspondence:  Karan Veer Singh, Tel: +91-01842267153, Fax: +91-01842267153, Email: karan.singh2@icar.gov.in
Received: 8 June 2021   • Revised: 29 July 2021   • Accepted: 7 December 2021
Abstract
Objective
The present study is aimed at phenotypic characterization and mitochondrial d-loop analysis of indigenous “Diara” buffalo population, which are mostly confined to the villages on the South and North Gangetic marshy plains in the Bihar state of India. These buffaloes are well adapted and are best suited for ploughing and puddling the wet fields meant for paddy cultivation.
Methods
Biometric data on 172 buffaloes were collected using a standard flexible tape measure. Animals are medium in size; the typical morphometric features are long head with a broad forehead and moderately long and erect ears. Genomic DNA was isolated from unrelated animals. The mtDNA d‐loop 358‐bp sequence data was generated and compared with 338 sequences belonging to riverine and swamp buffaloes.
Results
Based on the mitochondrial d-loop analysis the Diara buffaloes were grouped along with the haplotypes reported for riverine buffalo. Sequence analysis revealed the presence of 7 mitochondrial D loop haplotypes with haplotype diversity of 0.9643. Five of the haplotypes were shared with established swamp breeds and with Buffalo population of Orissa in India.

Conclusion

Morphometric analyses clearly shows distinguishing features like long and broad forehead which may be useful in identification. The germplasm of Diara buffalo is much adapted to the marshy banks of river Ganga and its tributaries. It constitutes a valuable genetic resource which needs to be conserved on priority basis.
Keywords: Buffalo; Genetic Diversity; Molecular Markers; Morphometric
TOOLS
METRICS Graph View
  • 0 Crossref
  •  0 Scopus
  • 121 View
  • 4 Download
Related articles


Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul 08776, Korea   
TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : animbiosci@gmail.com               

Copyright © 2022 by Animal Bioscience.

Developed in M2PI

Close layer
prev next