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Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.21.0272    [Accepted] Published online August 25, 2021.
Evaluation of recent changes in genetic variability in Thoroughbred horses based on microsatellite markers parentage panel in Korea
Chul Sung Park1  , Sun Young Lee2  , Gil Jae Cho1,* 
1College of Veterinary Medicine and Institute of Equine Medicine, Kyungpook National University, Daegu 41566, Korea
2Racing Laboratory, Korea Racing Association, Gwacheon 13822, Korea
Correspondence:  Gil Jae Cho, Tel: +82-53-950-5978, Fax: +82-53-950-5955, Email: chogj@knu.ac.kr
Received: 11 June 2021   • Revised: 30 July 2021   • Accepted: 12 August 2021
Abstract
Objective
In this study, we aimed to investigate the recent changes such as allele frequencies and total probability of exclusion (PE) in Thoroughbred horses in Korea using short tandem repeat (STR) parentage panels between 2006 and 2016.
Methods
The genotype was provided for 5,988 horse samples with 15 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3 and VHL20).
Results
In our study, the observed number of alleles per locus ranged from 3 (HMS1) to 9 (ASB17) in 2006 and 4 (HMS1) to 9 (ASB2) in 2016, with a mean value of 6.28 and 6.40, respectively. Of the 15 markers, HMS2, HTG4, and CA425 loci had relatively low polymorphism information content (<0.5000) in the Thoroughbred population. Mean levels of genetic variation in 2006 and 2016 were observed heterozygosity (HO) = 0.708, and expected heterozygosity (HE) = 0.685, as well as and HO = 0.699 and HE = 0.682, respectively. The PE was calculated for each group based on the allele frequencies of 14 or 15 STRs. The 2006 survey analyzed that PE was 0.9998, but it increased to 0.9999 in 2016 after the HMS2 marker was added in 2011. The current STR panel is still a powerful tool for parentage verification that contributes to the maintenance of integrity in the Thoroughbred population.

Conclusion

The current STR panel is still a powerful tool for parentage verification that contributes to the maintenance of integrity in the Thoroughbred horses. However, continuous monitoring genetic variability is necessary.
Keywords: Individual Identification; Microsatellite Marker; Parentage Verification; South Korea; Thoroughbred Horse


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