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Anim Biosci > Accepted Articles
https://doi.org/10.5713/ab.21.0151    [Accepted] Published online August 22, 2021.
Cysteine improves boar sperm quality via glutathione biosynthesis during the liquid storage
Zhendong Zhu1,2, Yao Zeng2, Wenxian Zeng2,*
1College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China
2Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Shaanxi, China
Correspondence:  Wenxian Zeng, Tel: +86-029-87092102, Fax: +86-029-87092102, Email: zengwenxian2015@126.com
Received: 31 March 2021   • Revised: 14 May 2021   • Accepted: 4 July 2021
Abstract
Objective
Sperm are particularly susceptible to reactive oxygen species (ROS) stress. Glutathione (GSH) is an endogenous antioxidant that regulates sperm redox homeostasis. However, it is not clear whether boar sperm could utilize cysteine for synthesis GSH to protect sperm quality from ROS damage. Therefore, the present study was undertaken to elucidate the mechanism of how cysteine is involved in protecting boar sperm quality during liquid storage.
Methods
Sperm motility, membrane integrity, lipid peroxidation, 4-hydroxyIlonenal (4-HNE) modifications, mitochondrial membrane potential, as well as the levels of ROS, GSH and, ATP were evaluated. Moreover, the enzymes (GCLC: glutamate cysteine ligase; GSS: glutathione synthetase) that are involved in glutathione synthesis from cysteine precursor were detected by western blotting.
Results
Compared to the control, addition of 1.25 mM cysteine to the liquid storage significantly increased boar sperm progressive motility, straight-line velocity (VSL), curvilinear velocity (VCL), beat-cross frequency (BCF), membrane integrity, mitochondrial membrane potential, ATP level, acrosome integrity, activities of superoxide dismutase (SOD) and catalase, and GSH level, while reducing the ROS level, lipid peroxidation and 4-HNE modifications. It was also observed that the GCLC and GSS were expressed in boar sperm. Interestingly, when we used menadione to induce sperm with ROS stress, the menadione associated damages were observed to be reduced by the cysteine supplementation. Moreover, compared to the cysteine treatment, the γ-GCS activity, GSH level, mitochondrial membrane potential, ATP level, membrane integrity and progressive motility in boar sperm were decreased by supplementing with an inhibitor of GSH synthesis, buthionine sulfoximine (BSO).
Conclusion
These data suggest that boar sperm could biosynthesize the GSH from cysteine in vitro. Therefore, during storage, addition of cysteine improves boar sperm quality via enhancing the GSH synthesis to resist ROS stress.
Keywords: Boar Sperm; Cysteine; GSH Synthesis; ROS Stress


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