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DOI: https://doi.org/10.5713/ajas.20.0545    [Accepted] Published online October 14, 2020.
Application of lactic acid bacteria producing antifungal substance and carboxylesterase on whole crop rice silage with different dry matter
Seong Shin Lee1  , Dimas Hand Vidya Paradhipta1,2  , Hyuk Jun Lee1  , Young Ho Joo1  , Hyeon Tak Noh1  , Jeong Seok Choi1  , Keum Bae Ji3  , Sam Churl Kim1,* 
1Division of Applied Life Science (BK21Plus, Insti. of Agri. & Life Sci.), Gyeongsang National University, Jinju 52828, Korea
2Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
3Institute of Technology, Livemac Co. Ltd., Gimje 54329, South Korea
Correspondence:  Sam Churl Kim, Tel: +82-55-772-1947, Fax: +82-55-772-1949, Email: kimsc@gnu.ac.kr
Received: 3 August 2020   • Revised: 9 September 2020   • Accepted: 5 October 2020
This study was conducted to investigate effects of antifungal substance and carboxylesterase-producing inoculant on fermentation indices and rumen degradation kinetics of whole crop rice (WCR) silage ensiled at different dry matter (DM) contents.
Dual-purpose inoculants, Lactobacillus brevis 5M2 and L. buchneri 6M1, confirmed both activities of antifungal and carboxylesterase in the previous study. The WCR at mature stage was chopped, and then wilted to obtain three different DM contents consisting of 35.4%, 43.6%, and 51.5%. All WCR forages were applied distilled water (CON) or mixed inoculants with 1:1 ratio at 1 × 105 cfu/g (INO), and ensiled into 20 L mini silo (5 kg) in quadruplicates for 108 d.
The INO silages had lower lactate (p<0.001) and butyrate (p=0.022) with higher acetate (p<0.001) and propionate (p<0.001) than those of CON silages. Ammonia-N (p<0.001), lactate (tendency; p=0.068), acetate (p=0.030), and butyrate (p<0.001) concentrations of INO silages decreased linearly with increasing DM content of WCR forage. The INO silages presented higher lactic acid bacteria (p<0.001) with lower molds (p<0.001) than those of CON silages. Yeasts (p=0.042) and molds (p=0.046) of WCR silages decreased linearly with increasing DM content of WCR forage. In the rumen, INO silages had higher the total degradable fraction (p<0.001), total volatile fatty acid (tendency; p=0.097), and acetate (p=0.007), but lower the fraction degradation rate (p<0.011) and propionate (p<0.001) than those of CON silage. The total degradable fraction (p<0.001), total volatile fatty acid (p<0.001), iso-butyrate (p=0.036), and valerate (p=0.008) decreased linearly with increasing DM content of WCR forage, while the lag phase (p<0.001) was increased linearly.
This study concluded that application of dual-purpose inoculants on WCR silage confirmed antifungal and carboxylesterase activities by inhibiting mold and improving rumen digestibility, while increase of wilting times decreased organic acids production and rumen digestibility.
Keywords: Haylage; Lactic Acid Bacteria; Rumen Fermentation; Silage; Whole Crop Rice
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