Profiling of skeletal muscle tissue for lncRNAs related to muscle metabolism in the QingYu pig at the growth inflection point

¹College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China
²College of Animal Science and Technology, Southwest University, Chongqing, China
³Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan, Chengdu, China
⁴Sichuan Province General Station of Animal Husbandry, Chengdu, China
⁵College of Life Science, Sichuan Agricultural University, Ya’an, China

Correspondence: Shunhua Zhang,Email: zhangsh1919@163.com
Li Zhu, Tel: 13982083385, Fax: 13982083385, Email: zhuli7508@163.com

Received: 22 June 2020 • Revised: 9 September 2020 • Accepted: 28 September 2020

Abstract

Objective
s: Investigation of muscle growth at different developmental stages is an appropriate strategy for studying the mechanisms underlying muscle development and differences in phenotypes. In particular, the muscle development mechanisms and the difference between the fastest and slowest growth rates. Methods: In this study, we used a growth curve model to fit the growth inflection point of QingYu pigs and compared differences in the lncRNA transcriptome of muscle both at the growth inflection point (IP) and plateau phase (PP). Results: The growth curve of the QingYu pig had a good fit (R2=0.974) relative to a typical S-curve and reached the IP at day 177.96. At the PP, marbling, intramuscular fat and monounsaturated fatty acids (MUFAs) had increased significantly and the percentage of lean muscle and polyunsaturated fatty acids (PUFAs) had decreased. A total of 1199 mRNAs and 62 lncRNAs were differentially expressed at the IP compared with the PP. Additional to Gene Ontology and KEGG pathway analyses, these differentially expressed protein coding genes were principally related to muscle growth and lipid metabolism. Conclusion: Our results suggest that the identified differentially expressed lncRNAs, could play roles in muscle growth, fat deposition and regulation of fatty acid composition at the IP and PP.

Keywords: lncRNA; Growth Curve; Inflection Point; Muscle; Metabolism; QingYu Pig