Ileal Endogenous Amino Acid Flow Response to Nitrogen-free Diets with Differing Ratios of Corn Starch to Dextrose in Pigs

C. Kong; D. Ragland; O. Adeola

doi:10.5713/ajas.2014.14232

Kong, Ragland, and Adeola: Ileal Endogenous Amino Acid Flow Response to Nitrogen-free Diets with Differing Ratios of Corn Starch to Dextrose in Pigs

Article

Asian-Australasian Journal of Animal Sciences (AJAS) 2014; 27(8): 1124-1130.

https://doi.org/10.5713/ajas.2014.14232

Ileal Endogenous Amino Acid Flow Response to Nitrogen-free Diets with Differing Ratios of Corn Starch to Dextrose in Pigs

C. Kong, D. Ragland¹, O. Adeola^*

¹Department of Veterinary Clinical Sciences, Purdue University, West Lafayette, IN 47907-2054, USA.

^*Corresponding Author: O. Adeola.
Tel: +1-765-494-4848, Fax: +1-765-494-9346, E-mail: ladeola@purdue.edu

Department of Animal Sciences, Purdue University, West Lafayette, IN 47907-2054, USA

Received March 31, 2014 Revised May 06, 2014 Accepted May 11, 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/ which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The objective of this study was to determine the responses in the digestibility of dry matter (DM) and amino acid (AA) composition of ileal endogenous flow (IEF) of pigs (initial body weight, 69.1±6.46 kg) fed N-free diets (NFD) formulated with different ratios of corn starch to dextrose. Fifteen pigs fitted with a T-cannula at the distal ileum were fed 5 diets according to a triplicated 5×2 incomplete Latin-square design. Each period consisted of a 5-d adjustment period and 2 d of ileal digesta collection for 12 h on each of d 6 and 7 and between each period, there was a 5-d recovery period to avoid abnormal weight loss. The ratios of corn starch to dextrose investigated were 0:879, 293:586, 586:293, 779:100, and 879:0 for diet numbers 1, 2, 3, 4 and 5, respectively, and chromic oxide (5 g/kg) was used as an indigestible index. Ileal DM digestibility was greater in Diet 1 than that in Diet 4 (89.5% vs 87.3%, p<0.01) but they were not different from Diet 2, 3, or 5. The IEF for most of indispensable AA were not different among diets with the exception of Met, in which a lack of corn starch or dextrose gave lower (p = 0.028) IEF of Met than diets containing corn starch and dextrose. Likewise, the dispensable AA and total AA in the IEF did not differ among diets. The respective IEF of AA (mg/kg of dry matter intake) in pigs fed Diets 1, 2, 3, 4, or 5 were 301, 434, 377, 477,or 365 for Lys, 61, 89, 71, 87, or 61 for Met, and 477, 590, 472, 520, or 436 for Thr. Proline was the most abundant AA in the IEF followed by Gly, Glu, and Asp and together accounted for approximately 50% of the total ileal AA flows of pigs fed NFD. In conclusion, the variation in proportion of corn starch and dextrose in a NFD does not largely affect estimates of IEF of N and AA for growing-finishing pigs.

Keywords: Ileal Digestibility; Basal Endogenous Amino Acid Loss; Swine

INTRODUCTION

Due to the existing limitations for apparent ileal digestibility including lack of additivity and representativeness of specific endogenous losses, respectively, there has been a growing consensus that standardized counterpart is more accurate data for estimating available amino acid (AA) contents in feed ingredients and diets for pigs (Stein et al., 2007). However, it should be considered that values for standardized ileal digestibility (SID) are affected by the estimates of basal endogenous losses (BEL) which may be dependent on several factors including the differences associated with feed ingredients, diet composition and mixing, and sampling and processing methods as well as analytical methods (Jansman et al., 2002; Stein et al., 2007). Depending on dietary AA intake, the relative proportion of basal endogenous loss to total ileal flow varies and increases when dietary AA intake decreases, indicating the difference in the apparent ileal digestibility (AID) of AA possibly caused by the variation of basal endogenous loss. Therefore, there has been a demand to measure the basal endogenous AA losses correctly and several methods have been suggested (Stein et al., 2007; Moughan and Rutherfurd, 2012). Due to the methodological simplicity, the N-free diet (NFD) feeding method has been commonly used for the estimation of basal endogenous AA losses in pigs and corn starch and dextrose or sucrose are widely used as major ingredients in NFD. Although there is a suggested composition of NFD, the ratio of corn starch to dextrose or sucrose varies across studies (Stein et al., 2007; Cozannet et al., 2010; Zhai and Adeola, 2011; Li et al., 2013). The variability in formulation of NFD may influence the estimates of the basal endogenous AA losses in pigs (Moughan and Rutherfurd, 2012) and consequently the values for SID of AA. For broilers, Kong and Adeola (2013) reported variations in the ratios of corn starch to dextrose affected estimates of ileal endogenous flows (IEF) of N and some AA. However, there is a scarcity of data on the variation in AA composition of BEL in pigs fed NFD varying the ratio of corn starch to dextrose. Thus, the objective of the current study was to determine the response in digestibility of dry matter (DM) and the AA composition of IEF of pigs fed NFD formulated with different ratios of corn starch to dextrose.

Materials and Methods

All animal procedures were approved by the Purdue Animal Care and Use Committee.

Pigs and management

A total of 15 crossbred (Hampshire×Duroc×Yorkshire ×Landrace) barrows with average initial body weight (BW) of 69.1±6.46 kg were surgically fitted with a simple T-cannula at the distal ileum as described by Dilger et al. (2004). All the pigs were housed in metabolism crates (1.22 by 1.22 m) equipped with low-pressure, automatic waterers and 12-h light-dark cycle.

Experimental diets were fed to 15 pigs according to a triplicated 5×2 incomplete Latin square design with each period lasting 7 d. There were 15 pigs in 3 replicates of 5 pigs per replicate fed the experimental diets over 2 periods of 7 d per period. Between each period, there was a 5-d recovery period in which pigs received a standard grower-finisher diet to avoid changes in physiology that may arise from prolonged feeding of NFD. Daily feed allowance was set as 3% of the BW of the lightest pig at the beginning of each period and was divided into 2 equal portions at 0700 and 1700 h. Ileal digesta were collected over 12 h on each of d 6 and 7. Collection bags were prefilled with 10 mL of 5% formic acid to reduce microbial proliferation, and digesta samples were stored at −20°C before processing. The digesta samples were subsequently thawed and pooled within pig in each period, and then subsampled, freeze dried, and ground before chemical analyses.

Diets

Ingredient composition of the diets is shown in Table 1. The experimental diets consisted of 5 NFD and the ratios of corn starch to dextrose investigated in the NFD were 0:879, 293:586, 586:293, 779:100, and 879:0 for diet numbers 1, 2, 3, 4, or 5, respectively. All diets contained calculated dietary electrolyte balance of 57.9 mEq/kg, and solka floc, soybean oil, monocalcium phosphate, MgO, limestone, NaCl, selenium premix, and K₂CO₃ at 20, 30, 30, 1, 5, 3, 0.5, and 4 g/kg, respectively. Vitamin and mineral premixes were supplemented according to NRC (1998) requirements and chromic oxide was incorporated into diets (5 g/kg) as an indigestible index.

Chemical analysis

Dietary and ileal digesta samples were analyzed for AA (method 982.30 E [a, b, c]; AOAC, 2006) and N (method 990.03; AOAC, 2006) at the University of Missouri Experiment Station Chemical Laboratory, USA. Duplicate proximate analyses were performed at Purdue University on diets and ileal digesta samples. Dry matter analysis of samples was performed by drying the samples in a drying oven at 100°C for 24 h (method 934.01; AOAC, 2006). Chromium concentration in the diets and ileal samples was determined using the method of Fenton and Fenton (1979).

Calculations

Apparent ileal digestibility (%) of DM and ileal endogenous N and AA flow (IEF, mg/kg of DM intake) in pigs were calculated using following equations (Kong and Adeola, 2013):

AID(%)=[1–(Cri/Cro)×(No/Ni)]×100IEF(mg/kg DMI)=(Cri/Cro)×(No)

where Cr_i is the concentration of chromium in the diet in g/kg DM intake; Cr_o is the concentration of chromium in the ileal digesta in g/kg DM output; N_o is the concentration of DM, N, or AA in the ileal digesta in mg/kg DM; N_i is the concentration of DM, N, or AA in the diet in mg/kg DM.

Statistical analysis

Apparent ileal DM digestibility and IEF of N and AA data were analyzed by using the MIXED procedure of SAS. The model consisted of Diet (1 to 5), collection period (1, 2), replicate (1 to 3), and pig (1 to 15) nested within replicate. Tukey’s honestly significant difference test was used to compare treatment means with an alpha level of 0.05.

Results

Nitrogen content in the Diet 1, 2, 3, 4, 5, corn starch, and dextrose was analyzed to be 0.5, 0.5, 0.6, 0.9, 0.9, 0.7, and 0.3 g/kg, respectively (Table 2). The analyzed individual AA content in the 5 experimental NFD was less than 0.6 g/kg of diet.

The apparent ileal DM digestibility and basal endogenous N and AA losses of pigs fed NFD with varying proportion of corn starch and dextrose are shown in Table 3. The apparent ileal DM digestibility was grater in Diet 1 than Diet 4 (89.5% vs 87.3%, p<0.01) but Diet 1 and 4 were not different from Diet 2, 3, or 5.

Ileal endogenous N flow (mg/kg of DMI) ranged from 1,776 (Diet 1) to 2,513 (Diet 4) and did not differ by various NFD. The IEF of all indispensable AA were not different among diets with the exception of Met, which was different among diets (p = 0.028) but their means were not separated by Tukey’s test. The respective IEF of AA (mg/kg of DMI) in pigs fed Diets 1, 2, 3, 4, or 5 were 301, 434, 377, 477,or 365 for Lys, 61, 89, 71, 87, or 61 for Met, and 477, 590, 472, 520, or 436 for Thr. Similar to the result for the IEF of indispensable AA, there was no difference in the IEF of dispensable and total AA among diets. Table 4 shows the ratio of individual AA to Lys in IEF of pigs fed NFD with different proportions of corn starch and dextrose. Met (0.17 to 0.21) and Pro (6.01 to 8.14) are the least and the most abundant AA in IEF for all the dietary treatments, respectively.

Discussion

The factors affecting the variation in the basal endogenous AA losses between and within laboratories have been described by several researchers (Jansman et al., 2002; Stein et al., 2007; Adedokun et al., 2011), which include the differences associated with feed ingredients, diet composition and mixing, and sampling and processing methods as well as analytical methods. Based on the reported possible variations caused by the structural differences between major ingredients of NFD (Kong and Adeola, 2013), the IEF of AA in pigs fed NFD with various corn starch to dextrose ratios were evaluated in the current study.

The analyzed individual AA contents of 5 NFD used in the current study ranged 0 to 0.6 g/kg which was a trace amount and close to the formulated value at 0 g/kg. Although there is a starch separating process which separates residual protein from starch by using multistep-hydrocyclone washing process, trace residual protein still exists (<0.5%) in the final product of corn starch (Corn Refiners Association, 2013).

The results obtained from the current study showed that AID of DM was greater in Diet 1 than Diet 4 but they were not different from Diet 2, 3, or 5. Because of the relative simple chemical structure of dextrose compared with corn starch (monosaccharide vs polysaccharide) and the digestion and absorption process of these major ingredients, it was hypothesized that a linear decrease in the AID of DM as the proportion of corn starch in NFD increased from 0 to 879 g/kg. Our previous broiler chicken study (Kong and Adeola, 2013) showed that the AID of DM decreased as the proportion of corn starch in NFD increased. The inconsistent results may be attributed to the differences in the digesta transit time between the 2 species. In general, pigs have a longer total tract digesta transit time than that of broilers when the transit time was determined with an indigestible marker (Kim et al., 2007; Wilfart et al., 2007; Hughes, 2008) and the transit time in pigs may be long enough to assimilate the corn starch in NFD to smaller molecules before absorption. In addition, the difference between the lowest and highest DM digestibility was determined to be 10.1% and 2.2% for broiler and pig studies, respectively. The average AID of DM (88.3%) in the current study was very close to the highest values determined in the previous study at 88.0%.

In the current study, the IEF of indispensable AA ranged from 61 (Met, Diet 1) to 590 mg/kg (Thr, Diet 2), which was close to the average range obtained in data from 34 reported studies that used ileal cannulated pigs and NFD to measure the IEF of crude protein and AA (Park et al., 2013). The results from the current study indicated that the variation in proportion of corn starch and dextrose in the experimental NFD did not affect the IEF of N and AA at the terminal ileum of growing-finishing pigs. This is not consistent with the results from the broiler study which showed the difference in the IEF of AA (Kong and Adeola, 2013). In the previous study, the inefficient digestive enzyme reutilization (Rothman et al., 2002) was speculated to explain the variation in the IEF of AA for broilers fed various NFD, however the speculation may not extend to pigs due to the relatively longer transition time of digesta in the digestive tract of pigs compared with broilers. Kong and Adeola (2013) showed the impact of variation in the IEF on the correction for the apparent ileal AA digestibility. Although the current study showed that there was no difference in the IEF of AA in pigs fed various NFD, there is still a considerable risk when the numerically different values (e.g., values from Diet 1 vs 4) are applied to correct the apparent ileal AA digestibility to standardized counterpart. Therefore, caution should be exercised when the values are practically used and it would be still worth having a standard NFD to minimize any possible variation in IEF of AA between studies until more information becomes available.

In the current study, the analysis of variance indicated that the IEF of Met differ by the variation of NFD, however Tukey’s test used for mean separation showed a lack of difference among the means because of the large variation in the data set. Park et al. (2013) showed that the BEL of Met had the greatest variability among experiments which used NFD to determine the BEL of AA. This may be due to the determination limit for Met in concert with the relatively low concentration of Met in the IEF compared with other AA.

Proline was the most abundant AA in the IEF followed by Gly, Glu, and Asp which was in agreement with the data reported from studies used growing-finishing pigs fed NFD (Zhang et al., 2002; Zhai and Adeola, 2011). Those abundant AA together accounted for 49.6, 51.6, 55.3, 55.8, and 56.9% of the total AA flows for Diets 1, 2, 3, 4, and 5, respectively, and these values were comparable to the results from previous studies (48.3%, Zhang et al., 2002; 57.2%, Zhai and Adeola, 2011). The sources of endogenous AA includes salivary and digestive secretion, epithelial cell turnover as well as mucin secretion in the gut (Nyachoti et al., 1997), thus the AA composition of the IEF would be dependent on the AA composition of these sources. Although there is an indication of the overestimation of IEF for Pro and Gly (de Lange et al., 1989; Moughan and Rutherfurd, 2012), Hofmann et al. (2010) and Bennick (1982) indicated that glycoproteins excreted with bile and saliva contain relatively large amounts of Gly and Pro. In addition, mucins accounted for approximately 11% of endogenous protein in ileal digesta of pigs with Thr contributing 30% to mucin protein (Lien et al., 1997). In the current study, Thr was the most abundant AA followed by Leu among indispensable AA regardless of the NFD used, and this was in agreement of the collective data derived from 34 research papers (Park et al., 2013). Moreover, the AA profile in relation to Lys in the IEF determined with 5 NFD in the current study was fairly comparable to the respective ratios determined with same methodology in the same laboratory (Zhai and Adeola, 2011) indicating that the ratio of AA to Lys in IEF may be stable with the same methodology regardless of the ratio of corn starch to dextrose in the NFD.

In conclusion, the results in the current study showed that the variation in the composition of a NFD, especially the proportion of corn starch and dextrose did not affect the IEF of AA in pigs. However, considering that the impact of variation in the IEF observed in the current study could be important when IEF is used in correcting AID, it is imperative to standardize the corn starch to dextrose ratio in NFD to minimize any possible variation in the IEF of AA across studies.

Table 1

Ingredient composition of the experimental diets, as-fed basis

Item	Dietary treatment No.
Item	1	2	3	4	5
Corn starch:dextrose	0:879	293:586	586:293	779:100	879:0
Ingredient (g/kg)
Corn starch	0	293	586	779	879
Dextrose	879	586	293	100	0
Solkafloc1	20	20	20	20	20
Soybean oil	30	30	30	30	30
Monocalcium phosphate	30	30	30	30	30
Limestone	5	5	5	5	5
NaCl	3	3	3	3	3
Selenium premix2	0.5	0.5	0.5	0.5	0.5
MgO	1	1	1	1	1
K₂CO₃	4	4	4	4	4
Vitamin premix3	1.5	1.5	1.5	1.5	1.5
Mineral premix4	1	1	1	1	1
Chromic oxide premix5	25	25	25	25	25
Total	1,000	1,000	1,000	1,000	1,000
Calculated composition
DE (kcal/kg)	3,216	3,403	3,591	3,715	3,779
ME (kcal/kg)	3,118	3,330	3,542	3,682	3,755
CP (g/kg)	1.4	2.2	3.0	3.6	3.9
Ca (g/kg)	6.55	6.55	6.55	6.55	6.55
nPP (g/kg)	6.30	6.30	6.30	6.30	6.30
Electrolyte balance, mEq6	57.9	57.9	57.9	57.9	57.9

DE, digestible energy; ME, metabolizable energy; CP, crude protein; nPP, non-phytate phosphorus.

¹ Purified cellulose (International Fiber Corp., North Tonawanda, NY, USA).

² Supplied 0.3 mg of Se per kilogram of diet.

³ Vitamin premix supplied per kilogram of diet: vitamin A, 2,423 IU; vitamin D₃, 242 IU; vitamin E, 17.6 IU; vitamin K activity, 2.4 mg; menadione, 804 μg; vitamin B₁₂, 14.1 μg; riboflavin, 2.8 mg; D-pantothenic acid, 9 mg; niacin,13 mg.

⁴ Mineral premix supplied per kilogram of diet: Cu (as copper sulfate), 9 mg; I (as calcium iodate), 0.34 mg; Fe (as ferrous sulfate), 97 mg; Mn (as manganese oxide), 12 mg; and Zn (as zinc oxide), 97 mg.

⁵ Prepared as 1 g chromic oxide added to 4 g solka floc.

⁶ Calculated as K+Na−Cl in mEq.

Table 2

Analyzed AA, and CP composition of the experimental diets and ingredients, as-fed basis

Item	Dietary treatment No.					Ingredient
Item	1	2	3	4	5	Cornstarch	Dextrose
Corn starch:dextrose	0:879	293:586	586:293	779:100	879:0
N (g/kg)	0.5	0.5	0.6	0.9	0.9	0.7	0.3
Indispensable AA (g/kg)
Arg	0.0	0.0	0.1	0.1	0.2	0.1	0.0
His	0.0	0.0	0.0	0.0	0.1	0.0	0.0
Ile	0.0	0.0	0.1	0.1	0.2	0.1	0.0
Leu	0.0	0.1	0.2	0.2	0.4	0.3	0.0
Lys	0.0	0.1	0.1	0.1	0.2	0.1	0.0
Met	0.0	0.0	0.0	0.0	0.1	0.1	0.0
Phe	0.0	0.1	0.1	0.1	0.2	0.2	0.0
Thr	0.0	0.0	0.1	0.1	0.1	0.1	0.0
Trp	0.3	0.3	0.3	0.3	0.3	0.0	0.0
Val	0.0	0.0	0.0	0.1	0.2	0.2	0.0
Dispensable AA (g/kg)
Ala	0.0	0.1	0.1	0.1	0.2	0.2	0.0
Asp	0.0	0.1	0.1	0.1	0.3	0.2	0.0
Cys	0.0	0.0	0.0	0.0	0.1	0.1	0.0
Glu	0.0	0.1	0.3	0.3	0.6	0.1	0.1
Gly	0.0	0.1	0.1	0.1	0.2	0.1	0.0
Pro	0.2	0.3	0.4	0.4	0.5	0.2	0.0
Ser	0.0	0.0	0.1	0.1	0.1	0.1	0.0
Tyr	0.0	0.0	0.1	0.1	0.1	0.1	0.0
Total AA (g/kg)	0.8	1.6	2.5	2.5	4.3	2.4	0.2

AA, amino acid; CP, crude protein.

Table 3

Ileal DM digestibility and ileal endogenous N and amino acid flow of pigs fed N-free diets with different proportions of corn starch and dextrose

Item	Dietary treatment					SEM	p-value
Item	1	2	3	4	5	SEM	p-value
Corn starch:dextrose	0:879	293:586	586:293	779:100	879:0
Ileal DM digestibility (%)	89.5a	87.9ab	88.0ab	87.3b	88.7ab	0.57	<0.01
Ileal endogenous flow (mg/kg of DMI)
N	1,776	2,207	2,045	2,513	1,925	231.4	0.065
Indispensable AA
Arg	338	454	404	577	413	70.3	0.103
His	138	192	162	183	159	23.2	0.279
Ile	246	333	273	319	250	32.2	0.092
Leu	438	589	473	550	420	64.3	0.157
Lys	301	434	377	477	365	67.0	0.282
Met	61a	89a	71a	87a	61a	8.2	0.028
Phe	253	344	275	322	245	35.8	0.120
Thr	477	590	472	520	436	55.9	0.226
Trp	128	146	148	166	137	11.6	0.126
Val	415	547	447	516	395	52.5	0.107
Dispensable AA
Ala	374	502	431	539	404	55.3	0.069
Asp	586	826	686	777	623	82.7	0.113
Cys	143	187	146	163	133	23.9	0.302
Glu	693	940	782	928	706	101.3	0.134
Gly	800	1,183	996	1,166	1,031	163.9	0.252
Pro	1,812	2,634	2,919	3,668	2,971	773.3	0.325
Ser	422	529	430	489	419	51.5	0.303
Tyr	214	291	234	272	210	31.4	0.173

DM, dry matter; SEM, standard error of the mean; DMI, dry matter intake; AA, amino acid.

^a,b Within a row, means without a common superscript differ (p<0.05).

Table 4

Ratio of individual AA to lysine in ileal endogenous flow of pigs fed N-free diets with different proportions of corn starch and dextrose1

Item	Current data					Published data
Item	1	2	3	4	5	Zhai and Adeola, 2011	Stein et al., 2005	Zhang et al., 2002 2
Corn starch:dextrose	0:879	293:586	586:293	779:100	879:0	768:100	784:100	686:200
Initial BW (kg)			69.1±6.46			47.1±1.0	92.1±3.19	65±1.85
Indispensable AA
Arg	1.12	1.04	1.07	1.21	1.13	1.04	0.92	1.00
His	0.46	0.44	0.43	0.38	0.43	0.39	0.39	0.39
Ile	0.82	0.77	0.73	0.67	0.69	0.63	0.86	0.74
Leu	1.45	1.36	1.26	1.15	1.15	1.08	1.35	1.42
Lys	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00
Met	0.20	0.21	0.19	0.18	0.17	0.15	0.24	0.58
Phe	0.84	0.79	0.73	0.68	0.67	0.63	0.80	0.84
Thr	1.58	1.36	1.25	1.09	1.19	1.00	1.12	1.65
Trp	0.42	0.34	0.39	0.35	0.38	0.24	0.20	1.29
Val	1.38	1.26	1.19	1.08	1.08	0.91	1.08	0.35
Dispensable AA
Ala	1.24	1.16	1.15	1.13	1.11	1.08	0.67	1.90
Asp	1.94	1.90	1.82	1.63	1.71	1.52	0.93	2.03
Cys	0.47	0.43	0.39	0.34	0.37	0.32	0.28	1.32
Glu	2.30	2.16	2.08	1.94	1.94	1.73	1.21	2.68
Gly	2.65	2.72	2.64	2.44	2.83	2.60	1.06	3.29
Pro	6.01	6.07	7.75	7.68	8.14	7.45	2.47	5.45
Ser	1.40	1.22	1.14	1.03	1.15	0.82	0.49	1.42
Tyr	0.71	0.67	0.62	0.57	0.57	0.51	0.35	0.48

AA, amino acid; BW, body weight.

¹ Ratios calculated as basal endogenous losses of individual AA divided by respective Lys loss.

² Corn starch:sucrose.

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