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Zhao, Chen, Yan, Liu, Zhang, Wang, Su, and Li: Effect of Sea Buckthorn Leaves on Inosine Monophosphate and Adenylosuccinatelyase Gene Expression in Broilers during Heat Stress

Abstract

The trial was conducted to evaluate the effects of sea buckthorn leaves (SBL) on meat flavor in broilers during heat stress. A total 360 one-day-old Arbor Acre (AA) broilers (male) were randomly allotted to 4 treatments with 6 replicates pens pretreatment and 15 birds per pen. The control group was fed a basal diet, the experimental group I, II and III were fed the basal diet supplemented with 0.25%, 0.5%, 1% SBL, respectively. During the 4th week, broilers were exposed to heat stress conditions (36±2°C), after which, muscle and liver samples were collected. High performance liquid chromatography (HPLC) was performed to measure the content of inosine monophosphate (IMP); Real-Time PCR was performed to determine the expression of the ADSL gene. The results showed that the content of breast muscle IMP of group I, II and III was significantly increased 68%, 102% and 103% (p<0.01) compared with the control, respectively; the content of thigh muscle IMP of group II and III was significantly increased 56% and 58% (p<0.01), respectively. Additionally, ADSL mRNA expression in group I, II and III was increased significantly 80%, 65% and 49% (p<0.01) compared with the control, respectively. The content of IMP and expression of ADSL mRNA were increased by basal diet supplemented with SBL, therefore, the decrease of meat flavor caused by heat stress was relieved.

INTRODUCTION

Sea buckthorn (Hippophae rhamnoides) is a small shrub comprised of fruit and leaves that are rich in nutrients and bioactive components such as vitamins (Kudritskaya et al., 1989; Zadernowski et al., 2003; Luhua et al., 2004; Ranjith et al., 2006), amino acids (Yushipitsina et al., 1988; Repyakh et al., 1990), lipids (Goncharova and Glushenkova, 1993; Ul’chenko et al., 1995; Bekker and Giushenkova, 1997), sugars and acids (Yang, 2009), and flavonoids (Häkkinen et al., 1999). Recently studies have shown that sea buckthorn has antioxidant (Geetha et al., 2002a; Geetha et al., 2002b; Chawla et al., 2007; Püssa et al., 2007; Geetha et al., 2009), anticancer (Olsson et al., 2004; Ma et al., 2007; Boivin et al., 2007), antiinfective (Larmo et al., 2008) and exerts beneficial effects on liver fibrosis (Gao et al., 2003) and immune function (Dorhoi et al., 2006). Sea buckthorn decreases the damage of enzyme activity or hormonal metabolic organism status caused by immobilization or cold-hypoxia-restraint (Krylova et al., 2000; Saggu and Kumar, 2007), however, its effects on heat stress is unclear. Heat stress is one of the most universal stress factors affecting poultry (Gregory, 2010), and is a major contributor to growth restriction (Liu, 2011; Melesse et al., 2011). In addition, heat stress can also influence the meat quality and flavor of poultry (Miao, 2007; Chiang et al., 2008; Lu et al., 2009).
Muscle inosine monophosphate (IMP) content is an indicator meat flavor and adenylosuccinatelyase (ADSL) is a key enzymes regulating IMP synthesis. Zhang et al. (2006) indicated there was positive correlation between the expression of ADSL gene and muscle IMP content. Therefore, the goal of the current study was to assess the effect of sea buckthorn leaves (SBL) on ADSL gene expression and IMP content were investigated during heat stress in poultry.

MATERIALS AND METHODS

Experimental birds, diets, and treatment

A total of 360 one-day-old male AA broilers (Harbin Delin broiler Co., Harbin, China) were randomly allotted to 4 groups of 90 birds each, with 6 replicates of 15 birds each. SBL that was collected in the Farm 8511 in June (Mishan, China) was shattered into powder with passing 40 screen mesh. The control group was fed a basal diet while the experimental group I, II and III were fed the basal diet supplemented with 0.25%, 0.5%, 1% SBL powder, respectively. Small amounts of the basal diet were first mixed with the respective amounts of SBL powder as a small batch and then mixed with a larger amount of the basal diet until the total amounts of the respective diets were homogenously mixed. The basal maize-soybean diet (Table 1) was formulated to refer to the NY/T 33-2004 (Chinese, 2004) and NRC (1994) nutrient requirement. Diets and fresh water were offered ad libitum. During the 4th week (from the 22th day to the 28th day), birds were exposed to heat stress at 36±2°C for 12 h/d (06:00 to 18:00) and 22°C for 12 h/d (18:00 to 06:00).

Sampling time and procedures

At the 29th day, 2 birds per replicate were randomly killed after fasting 12 h. by cervical dislocation. The carcasses were then opened and the left breast and thigh muscle and liver samples were rapidly harvested and stored at −80°C until analysis. The Committee on Animal Care at Northeast Agricultural University approved the animal care procedures employed.

Assay of IMP content

Assay of IMP content in muscles

One gram of muscle sample was shredded and homogenated two times in chilled 10 ml 3.5% HClO4. The resulting homogenate was centrifuged at 3,500×g for 10 min and the supernatant was collected, the sediment was washed in 5 ml 3.5% HClO4, centrifuged and the supernatant collected and combined with the first supernatant. Double distilled water (ddH2O) was added at twice the volume of the supernatant up to 25 ml. The diluted supernatant was pipetted in 1 ml aliquots and 9 ml distilled water was added. Samples were filtered by 0.45 μm filter membrane and separated by high-performance liquid chromatography (HPLC) equipped with the 600E Multisolvent Delivery System and a UV 2487 Detector with Empower software (Waters Co., Milford, USA). The chromatographic column was Symmetry Shield™ RP18 (C18), 5 μm (Waters Co., Milford, USA). Mobile phase was phosphate triethyl ammonium and methanol (Sigma Co., USA) (95:5), all reagents were chromatographic pure; the flow rate was 1.0 ml/min; the detection wavelength was 254 nm; the chromatographic column temperature was 25°C; the sample size was 10 μl.

Preparation of standard curve

The IMP standard substance (Sigma chemicals, St. Louis, USA) was accurately weighed and then dissolved in the appropriate volume of Mobile phase to produce corresponding standard solutions at six concentrations in the range of 0.25 to 8 μg/ml (multiproportion). The IMP standard curves were plotted after linear regression of the peak areas versus concentrations.

Assay of gene expression

Assay of ADSL gene expression in liver

Total RNA was extracted from liver tissue, referring to the method offered by Shanghai Huashun RNArose kit (Huashun biotechnology Co., Shanghai, China). The RNA quality was detected by formaldehyde gel apomorphosis electrophoresis, the RNA concentration and purity was determined by spectrophotometer at A260/A280, and 2 μg total RNA was reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega Co., Madison, USA). ADSL primers were synthesized by Shanghai biotechnology Co. (China), referring to the GeneBank cDNA sequence (Table 2), and the reference gene was 18sRNA (Yusuke Matsubara et al., 2005).
Amplicons via 1% agarose gel electrophoresis (AGE) chosen for sequencing were purified by using a TIANgel Midi Purification Kit (Tiangen biotechnology Co., Beijing, China) prior to sequencing. After purification, the PCR product was sequenced by Shanghai biotechnology Co. (China), and the sequencing primer sets were the same as those used for Real-Time PCR. Target genes were determined by Real-Time PCR after validating the sequence homologies.
Real-Time PCR procedure referred to SYBR® Premix Ex Taq™ kit instruction obtained from TaKaRa biotechnology Co. (Dalian, China), and 1 μl cDNA template was introduced into the reaction that was carried out in 20 μl ABI PRISM 7500 Real-Time PCR Reaction System (Applied Biosystems Co., USA). Temperature cycles were as follows: 95°C pre-degeneration 10 s, 95°C degeneration 5 s, 60°C renaturation/extension 34 s.

Preparation of amplification efficiency curve

The cDNA was gradient diluted according to Table 3, and various concentrations of cDNA were amplified by Real-Time PCR with the primers of ADSL and 18sRNA genes. The reaction system and procedure were in accordance to above. The amplification efficiency curves were plotted after linear regression of the cycle threshold (Ct value) versus Log 10diluion. Amplification efficiency E = 10−1/slope-1.

Statistical analyses

Data was analyzed by using one-factorial ANOVA procedure of SAS 8.0. Statistical significance was assumed at p<0.01. Data were expressed as mean±pooled SEM. Replicate was considered as the experimental unit. Number (n) used for statistics is note in the tables.

RESULTS

Effects of SBL on muscle IMP content in AA broilers

The effects of SBL on muscle IMP content are summarized in Table 3. Compared with the control, the content of breast muscle IMP of group I, II and III was significantly increased 68, 102, and 103% (p<0.01), respectively; the content of thigh muscle IMP of group II and III was significantly increased 56, and 58% (p<0.01), respectively (Table 4), but there was no significant influence in group I (p>0.05).

Effects of SBL on ADSL mRNA expression in liver in AA broilers

The regression equation of ADSL gene amplification curve is as follows: y = −3.2246x+32.776, R2 = 0.9972; the regression equation of 18sRNA gene amplification curve is as follows: y = −3.2025x+33.081, R2 = 0.9976. The amplification efficiency of the target gene is similar to that of reference gene from amplification efficiency E. The ADSL gene relative expression was expressed as 2−ΔCt (Feng, 2007), and its gene relative expression of group I, II and III were significantly increased 80, 65, and 49% (p<0.01), respectively, compared to chicks fed basal diet (Table 4).

DISCUSSION

Heat stress is one of the most universal and predominant factors that restrict the development of poultry in modern cultivation facilities. A number of bioactive components have been used to reduce heat stress, including tea polyphenols (Wang, 2004; Liu, 2005), extraction of fructus ligustri lucidi (Zhuang, 2007), Chinese magnoliavine fruit (Ma et al., 2005), lycopene (Sahin et al., 2006), and Turkish propolis (TatlıSeven et al., 2007). It has been reported that vitamin E, vitamin C and flavones also reduce the detrimental effects of heat stress (Sahin et al., 2002a; Sahin et al., 2002b; Chen, 2004; Wang, 2004; Huang, 2005). These are major constituents of sea buckthorn, however, there has been no direct study of the anti-heat stress of sea buckthorn. Heat stress not only decreases poultry performance (Chiang et al., 2008), but also the meat flavor. Meat flavor is influenced by flavor precursors, including amino acids, carbohydrates, inorganic salts and nucleotides. ATP is the major nucleotide in living muscle, however, during rigor mortis, it is converted into IMP. IMP is thought to be one of the most important factors in flavor development as it is a source of reducing sugar and nitrogenous base for Maillard reactions and acts as a flavor enhancer (Bayliss et al., 1995). Herein, SBL as a feed additive decreased the damage to meat flavor by increasing the muscle IMP content, but the interaction between IMP and other meat flavor precursors and its organoleptic properties remains to be determined.
One of the mechanisms of action whereby SBL increased IMP content was investigated. ADSL is an important enzyme that catalyzes two similar reactions in the de novo purine biosynthesis pathway. It catalyses the conversion of succinylaminoimidazole carboxamide ribotide (SAICAR) to aminoimidazole carboxamide ribotide (AICAR), as well as, the formation of adenosine monophosphate (AMP) from adenylosuccinate in the purine nucleotide cycle (Kmoch et al., 2000; Toth and Yeates, 2000). The content of IMP in muscle is influenced by pH, temperature and water activity (Vani et al., 2005; Kavitha et al., 2006), however, the background in raw meat is determined by ADSL gene expression (Zhang et al., 2006). All three SBL levels increased the ADSL gene expression. In a previous study, estrogen significantly increased the transcription rate of the ADSL gene (Zhu et al., 2001). The flavones in sea buckthorn are phytoestrogen that can exert estrogenic or anti-estrogenic actions depending on concentration, so it is possible that the SBL increased the ADSL gene expression in liver via its estrogenic activity. Generally, the SBL increased muscle IMP content by increasing ADSL gene expression, but the basal diet supplemented with 0.25% SBL didn’t increase the IMP content of thigh muscle although it increased ADSL gene expression, implying that dosage is an important factor. In addition, Kavitha and Modi (2006) found that the IMP content of fresh breast muscle was higher than that of fresh thigh muscle, suggesting that the synthesis of IMP in the breast muscle was different from thigh muscle maybe or that other ingredients of SBL played a role in part in the deposition of IMP. There are currently no data on the effect of SBL and the underlying mechanism of its action. In light of these results, future studies will evaluate the other mechanisms of SBL involved in improving meat flavor during regular temperature and heat stress.
In a summary, a basal diet supplemented with SBL increased muscle IMP content of AA broilers compared with the control, thereby, decreased the loss of meat flavor substance caused by heat stress. The likelihood was that SBL played a part in improving meat flavor during heat stress in AA broilers via enhancing the ADSL gene expression in livers.

ACKNOWLEDGEMENTS

The investigation was supported by the Special Fund for Technological Innovation Talents of Harbin Science and Technology Bureau (2011RFLXN015), the Program for Backbone Teachers Innovation Ability of Heilongjiang Province (1152G008), and the Program for Innovative Research Team of Northeast Agricultural University (IRTNEAU, CXT006-2-1).

Table 1
Composition and nutrient level of basal diet (air-dry basis)
Items 0–3 weeks 4 weeks
Ingredients (%)
 Corn 60.45 63.90
 Soybean meal 32.05 28.50
 Maize oil 2.50 3.20
 Fish meal 1.10 1.10
 DL-met 0.21 0.09
 Lys 0.03 0.00
 CaHPO4 1.80 1.60
 Limestone 1.33 1.13
 NaCl 0.20 0.15
 Vitamins premix1 0.03 0.03
 Minerals premix2 0.20 0.20
 Choline chloride 0.10 0.10
 Total4 100.00 100.00
Nutrient levels3
 ME (MJ/kg) 12.56 12.96
 CP (%) 21.53 20.01
 Lys (%) 1.15 1.03
 Met (%) 0.55 0.41
 Met+cys (%) 0.91 0.76
 Ca (%) 0.91 0.76
 Total P (%) 1.02 0.90
 Available P (%) 0.70 0.65

1 Provided per kg diet: vitamin A, 8,000 IU; vitamin D3, 1,000 IU; vitamin E, 20 IU; vitamin K, 0.5 mg; vitamin B1, 2.0 mg; vitamin B2, 8 mg; vitamin B12, 0.01 mg; bioepiderm, 0.18 mg; pantothenic acid, 10 mg; niacin, 35 mg; pyridoxine, 3.5 mg; folic acid, 0.55 mg; choline, 1,300 mg supplied by Lovit broiler I complex vitamins (Harbin Lovit animal health Co., Harbin, China).

2 Provided per kg diet: Cu, 8 mg; Mn, 120 mg; Fe, 100 mg; Zn, 100 mg; Se, 0.30 mg; I, 0.7 mg supplied by Luoman broiler H complex minerals (Harbin Lovit animal health Co., Harbin, China).

3 Metabolic energy and crude protein were calculated values.

4 Basal diet (Total 100) with additional SBL powder.

Table 2
Primer sequence, product size and position
Gene Primers Product/bp GenBank No.
ADSL F: 5′-TTGGCTCAAGTGCTATGC-3′
R: 5′-TCCCTCGGAGATGTTCTG-3′
224 bp EU049886
18sRNA F: 5′-TAGATAACCTCGAGCCGATCGCA-3′
R: 5′-GACTTGCCCTCCAATGGATCCTC-3′
312 bp AF173612
Table 3
Effect on IMP content of breast muscle and thigh muscle1,2 unit (mg/g)
Items Control Group I Group II Group III SEM p
breast muscle 1.65c 2.77b 3.34a 3.35a 0.078 <0.001
thigh muscle 1.98b 2.18b 2.57a 2.61a 0.084 0.0012

1 Values are mean±pooled SEM (n = 6).

2 Means without a common letter differ, p<0.01.

Table 4
Effect of SBL on ADSL mRNA expression1,2
Items Control Group I Group II Group III SEM p
Liver 0.0113b 0.0203a 0.0186a 0.0168a 0.00176 0.0050

1 Values are mean±pooled SEM (n = 6).

2 Means without a common letter differ, p<0.01.

REFERENCES

Bayliss P. 1995. Chemistry in the kitchen: the chemistry of flesh foods III. Nutr Food Sci 95:23–28.
crossref
Bekker NP, Giushenkova AI. 1997. Neutral lipids of the bark of Hippophae rhamnoides branches. Chem Nat Compd 29:493.
crossref
Boivin D, Blanchette M, Barrette S, Moghrabi A, Béliveau R. 2007. Inhibition of cancer cell proliferation and suppression of TNF-induced activation of NFkappaB by edible berry juice. Anticancer Res 27:937–948.
pmid
Chawla R, Arora R, Singh S, Sagar RK, Sharma RK, Kumar R, Sharma A, Gupta ML, Singh S, Prasad J, Khan HA, Swaroop A, Sinha AK, Gupta AK, Tripathi RP, Ahuja PS. 2007. Radioprotective and antioxidant activity of fractionated extracts of berries of Hippophae rhamnoides. J Med Food 10:101–109.
crossref pmid
Chen RG, Lochmann R, Goodwin A, Praveen K, Dabrowski K, Lee KJ. 2004. Effects of dietary vitamin C and E on alternative complement activity, hematology, tissue composition, vitamin concentrations and response to heat stress in juvenile golden shiner (Notemigonus crysoleucas). Aquaculture 242:553–569.
crossref
Chiang W, Booren A, Strasburg G. 2008. The effect of heat stress on thyroid hormone response and meat quality in turkeys of two genetic lines. Meat Sci 80:615–622.
crossref pmid
Dorhoi A, Dobrean V, Zăhan M, Virag P. 2006. Modulatory effects of several herbal extracts on avian peripheral blood cell immune responses. Phytother Res 20:352–358.
crossref pmid
Feng YM. 2007. Effects of excessive vitamin A on calcium and phosphorous metabolism in broilers and underlying mechanisms. Ph. D. Thesis. Inner Mongolia Agricultural University; Hohhot, Inner Mongolia:

Gao ZL, Gu XH, Cheng FT, Jiang FH. 2003. Effect of sea buckthorn on liver fibrosis: a clinical study. World J Gastroenterol 9:1615–1617.
crossref pmid pmc
Geetha S, Sai Ram M, Sharma SK, Ilavazhagan G, Banerjee PK, Sawhney RC. 2009. Cytoprotective and antioxidant activity of seabuckthorn (Hippophae rhamnoides L.) flavones against tert-butyl hydroperoxide-induced cytotoxicity in lymphocytes. J Med Food 12:151–158.
crossref pmid
Geetha S, Sai Ram M, Singh V, Ilavazhagan G, Sawhney RC. 2002a. Anti-oxidant and immunomodulatory properties of seabuckthorn (Hippophae rhamnoides)-an in vitro study. J Ethnopharmacol 79:373–378.
crossref pmid
Geetha S, Sai Ram M, Singh V, Ilavazhagan G, Sawhney RC. 2002b. Effect of seabuckthorn on sodium nitroprusside-induced cytotoxicity in murine macrophages. Biomed Pharmacother 56:463–467.
crossref pmid
Goncharova NP, Glushenkova AI. 1993. Lipids of the leaves of Central Asian forms of sea buckthorn. Chem Nat Compd 33:797–798.
crossref
Gregory NG. 2010. How climatic changes could affect meat quality. Food Res Int 43:1866–1873.
crossref
Häkkinen SH, Kärenlampi SO, Heinonen IM, Mykkänen HM, Törrönen AR. 1999. Content of the flavonols quercetin, myricetin, and kaempferol in 25 edible berries. J Agric Food Chem 47:2274–2279.
crossref pmid
Huang JM, Wang GL, Liu ZB, Zhang LJ. 2006. Effect of daidzein on testicle development, sperm quality and serum thyroid hormone of cock under acute heat-stress condition. Reprod Phys 42:23–25.

Kavitha S, Modi VK. 2006. Effect of water activity and temperature on degradation of 5′-inosine monophosphate in a meat model system. LWT-Food Sci Tech 40:1280–1286.
crossref
Kmoch S, Hartmannová H, Stibùrková B, Krijt J, Zikánová M, ebesta I. 2000. Human adenylosuccinate lyase (ADSL), cloning and characterization of full-length cDNA and its isoform, gene structure and molecular basis for ADSL deficiency in six patients. Hum Mol Genet 9:1501–1513.
crossref pmid
Krylova SG, Konovalova ON, Zueva EP. 2000. Correction by common sea buckthorn bark and sprout extracts of hormonal and metabolic disturbances during stress in rats. Eksp Klin Farmakol 63:70–73.

Kudritskaya SE, Zagorodskaya LM, Shishkina EE. 1989. Carotenoids of the sea buckthorn, variety Obil’naya. Chem Nat Compd 25:724–725.
crossref
Larmo P, Alin J, Salminen E, Kallio H, Tahvonen R. 2008. Effects of sea buckthorn berries on infections and inflammation: a double-blind, randomized, placebo-controlled trial. Eur J Clin Nutr 62:1123–1130.
crossref pmid
Liu HJ. 2005. Eeffct of TEA Poly Phenols and Daidein broilers under heat-stress condition. Master’s Thesis. Northeast Agricultural University; Harbin, Heilongjiang:

Liu M. 2011. Effects of acute heat stress on growth performance and lipid metabolism of broilers. Chinese Journal o f Animal Nutrition 23:862–868.

Luhua Z, Ying T, Zhengyu Z, Guangji W. 2004. Determination of alpha-tocopherol in the Traditional Chinese Medicinal preparation Sea buckthorn oil capsule by non-aqueous reversed phase-HPLC. Chem. Pharm. Bull. (Tokyo) 52:150–152.
crossref pmid
Lu QP, Wen J, Zhang HF, Dong YW, Wang QJ. 2009. Effects of high ambient temperature on meat quality and flavor in commercial and local broilers. Acta Veterinaria et Zootechnica Sinica 40:203–207.

Ma DY, Shan AS, Chen ZH. 2005. Influence of Ligustrum Lucidum, Schisandra Chinensis, Si Jun Zi Tang and Daidzein on Antioxidant Staus of Laying Hens Under Heat Stress. Acta Zoonutrimenta Sinica 17:23–27.

Ma G, Yang CL, Qu Y, Wei HY, Zhang TT, Zhang NJ. 2007. The flavonoid component isorhamnetin in vitro inhibits proliferation and induces apoptosis in Eca-109 cells. Chem Biol Interact 167:153–160.
crossref pmid
Matsubara Y, Sato K, Ishii H, Akiba Y. 2005. Changes in mRNA expression of regulatory factors involved in adipocyte differentiation during fatty acid induced adipogenesis in chicken. Comp Biochem Phys A 141:108–115.
crossref
Melesse A, Maak S, Schmidt R, von Lengerken G. 2011. Effect of long-term heat stress on some performance traits and plasma enzyme activities in Naked-neck chickens and their F1 crosses with commercial layer breeds. Livest Sci
crossref pmid pmc
Miao Y. 2007. Effects of acute heat stress on meat quality and peroxidation in broilers fed different sources of fat. Master’s Thesis. Chinese Aeademy of Agricultural Sciences; Beijing:

NY/T 33-2004 (Agriculture Standard of The People’s Republic of China). 2004. Feeding standard of chicken. Ministry of Agriculture of The People’s Republic of China; Beijing:

National Research Council. 1994. Nutrient requirements of poultry. 9th EdNational Academy Press; Washington, DC:

Olsson ME, Gustavsson KE, Andersson S, Nilsson A, Duan RD. 2004. Inhibition of cancer cell proliferation in vitro by fruit and berry extracts and correlations with antioxidant levels. J Agric Food Chem 52:7264–7271.
crossref pmid
Püssa T, Pällin R, Raudsepp P, Soidla R, Rei M. 2007. Inhibition of lipid oxidation and dynamics of polyphenol content in mechanically deboned meat supplemented with sea buckthorn (Hippophae rhamnoides) berry residues. Food Chem 107:714–721.
crossref
Ranjith A, SarinKumar K, Venugopalan VV, Arumughan C, Sawhney RC, Virendra Singh. 2006. Fatty acids, tocols, and carotenoids in pulp oil of three sea buckthorn species (Hippophae rhamnoides, H. salicifolia, and H. tibetana) grown in the Indian himalayas. J Am Oil Chem 83:359–364.
crossref
Repyakh SM, Kargapol’tsev AP, Chuprova NA, Yushipitsina GG. 1990. Amino acid composition and biological value of proteins of the woody verdure of sea buckthorn. Chem Nat Compd 26:110–111.
crossref
Saggu S, Kumar R. 2007. Possible mechanism of adaptogenic activity of seabuckthorn (Hippophae rhamnoides) during exposure to cold, hypoxia and restraint (C-H-R) stress induced hypothermia and post stress recovery in rats. Food Chem Toxicol 45:2426–2433.
crossref pmid
Sahin K, Ondercib M, Sahinb N, Gursuc MF, Khachikd F, Kucuke O. 2006. Effects of lycopene supplementation on antioxidant status, oxidative stress, performance and carcass characteristics in heat-stressed Japanese quail. J Therm Biol 31:307–312.
crossref
Sahin K, Sahin N, Onderci M. 2002a. supplementation can alleviate negative effects of heat stress on egg production, egg quality, digestibility of nutrients and egg yolk mineral concentrations of Japanese quails. Res Vet Sci 73:307–312.
crossref pmid
Sahin K, Sahin N, San M, Gursu MF. 2002b. Effects of vitamins E and A supplementation on lipid peroxidation and concentration of some mineral in broilers reared under heat stress (32°C). Nutr Res 22:723–731.
crossref
TatliSeven P, Seven I, Yilmaz M, Şimşek ÜG. 2007. The effects of Turkish propolis on growth and carcass characteristics in broilers under heat stress. Anim Feed Sci Technol 146:137–148.
crossref
Toth EA, Yeates TO. 2000. The structure of adenylosuccinate lyase, an enzyme with dual activity in the de novo purine biosynthetic pathway. Structure 8:163–174.
crossref pmid
Ul’chenko NT, Zhmyrko TG, Glushenkova AI, Murdakhaev YM. 1995. Lipids of Hippophae rhamnoides pericarp. Chem Nat Compd 31:565–567.
crossref
Vani ND, Modi VK, Kavitha S, Sachindra NM, Mahendrakar NS. 2005. Degradation of inosine-5′-monophosphate (IMP) in aqueous and in layering chicken muscle fibre systems: Effect of pH and temperature. LWT-Food Sci Technol 39:627–632.
crossref
Wang YQ. 2004. Eeffet of tea polyphenols and vitamin E on borilers performance and antioxygen on under heat-srtess condtions. Master’s Thesis. Northeast Agricultural University; Harbin, Heilongjiang:

Yang B. 2009. Sugars, acids, ethyl β-D-glucopyranose and a methyl inositol in sea buckthorn (Hippophae rhamnoides) berries. Food Chem 112:9–97.
crossref
Yushipitsina GG, Chuprova NA, Repyakh SM. 1988. Fractionation and amino acid compositon of proteins of the woody verdure of sea buckthorn. Chem Nat Compd 24:348–350.
crossref
Zadernowski R, Naczk M, Amarowicz R. 2003. Tocopherols in sea buckthorn (hippophaë rhamnoides l.) Berry oil. J Am Oil Chem Soc 80:55–58.
crossref
Zhang XY, Ji CL, Chen GH, Qin J, Shu JT, Su YJ. 2006. The diversity of adenylosuccinate lyase (Ads1) gene and its relationship with Inosinic acid content in chicken. J Yunnan Agr Univ 21:231–234.

Zhu YF, Wang M, Lin H, Li Z, Luo J. 2001. Identification of estrogen-responsive genes in chick liver. Cell Tissue Res 305:357–363.
crossref pmid
Zhuang BF. 2007. Influence of extraction of fruetus ligustri lueidi on meat quality and incretion status of broilers under heat stress. Master’s Thesis. Northeast Agricultural University; Harbin, Heilongjiang:

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