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Relationship between Developmental Ability and Cell Number of Day 2 Porcine Embryos Produced by Parthenogenesis or Somatic Cell Nuclear Transfer |
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Sang Jun Uhm, Mukesh Kumar Gupta, Hak-Jae Chung, Jin Hoi Kim, Chankyu Park, Hoon Taek Lee* |
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Correspondence:
Hoon Taek Lee,
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| Abstract |
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In vitro produced porcine embryos have potential application in reproductive biotechnology. However, their development potential has been very low. This study evaluated the in vitro developmental ability and quality of cloned and parthenogenetic porcine embryos having 2-4 cells or 5-8 cells on Day 2 of in vitro culture. Analysis of results showed that 2 to 4 cell embryos had higher ability to form blastocysts than 5 to 8 cell embryos (p<0.05). Blastocysts produced from culture of 2 to 4 cell embryos also contained higher cell numbers and had lower BAX:BCLxL transcript ratio than those produced from 5 to 8 cell embryos (p<0.05), thereby suggesting 2 to 4 cell embryos have higher development potential. Further investigation revealed that 5 to 8 cell embryos had higher incidence (100??.0%) of blastomeric fragmentation than 2 to 4 cell embryos (15.2??.5% for parthenogenetic and 27.7??.1% for cloned embryos). This suggests that low development potential of 5 to 8 cell embryos was associated with blastomeric fragmentation. In conclusion, we have shown that morphological selection of embryos based on cell number on Day 2 of in vitro culture could offer a practical and valuable non-invasive means to select good quality porcine embryos. |
| Keywords:
Blastomeric Fragmentation; Developmental Ability; Embryo Quality; Parthenogenesis; Porcine; Somatic Cell Nuclear Transfer |
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