Cloning of Chicken Microsomal Glutathione S-transferase 1 Gene (MGST1) and Identification of Its Different Splice Variants

Wang, X.-T.; Zhang, H.; Zhao, C.-J.; Li, J.-Y.; Xu, G.-Y.; Lian, L.-S.; Wu, C.-X.; Deng, X.-M.

doi:2009.22.2.155

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 2009;22(2): 155-161.
https://doi.org/10.5713/ajas.2009.80166 Published online January 6, 2009.

Cloning of Chicken Microsomal Glutathione S-transferase 1 Gene (MGST1) and Identification of Its Different Splice Variants

X.-T. Wang, H. Zhang, C.-J. Zhao, J.-Y. Li, G.-Y. Xu, L.-S. Lian, C.-X. Wu, X.-M. Deng^*

Correspondence: X.-M. Deng,

Abstract

Mammal microsomal glutathione transferase 1 (MGST1) can conjugate many toxic or carcinogenic substances and depress oxidative stress. In this study, Chicken MGST1 and its variants were cloned for the first time and were composed of 956 or 944 nucleotides. The 12 nt deletion in the exon 2 did not alter the GT-AG rule and the ORFs for the two MGST1 variants were the same, which both comprised 465 nucletides and encoded a peptide with 155 amino acids. It was found that the two different splice variants identified using RT-PCR expressed in all three organs investigated of Dwarf Brown Chicken, namely liver, spleen and shell gland. Moreover, the expression level of MGST1 mRNA in the liver of Dwarf Brown chickens was the highest (p<0.01), and there were no significant differences between the spleen and the shell gland. These results provide a base for studying the biological function of Chicken MGST1.

Keywords: Chicken; MGST1; RACE; Splice Variant