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Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 2008;21(7): 972-979.
DOI: https://doi.org/10.5713/ajas.2008.70534    Published online July 1, 2008.
An Antisense Oligodeoxynucleotide to the LH Receptor Attenuates FSH-induced Oocyte Maturation in Mice
Jiange Yang, Maoyong Fu, Songbo Wang, Xiufen Chen, Gang Ning, Baoshan Xu, Yuzhen Ma, Meijia Zhang, Guoliang Xia*
Correspondence:  Guoliang Xia,
It has been recently shown that expression of the LH receptor (LHR) in cumulus cells is related with FSH-induced meiotic resumption of mouse cumulus enclosed oocytes (CEOs). However, to date, it is still unclear whether LHR expression in cumulus cells plays a key role during FSH-induced oocyte maturation. The purpose of this study was to characterize the functional role of LHRs in cumulus cells. CEOs were isolated from eCG-primed preovulatory follicles and cultured in hypoxanthine (HX) arrested medium. LHR protein expression in cumulus cells was time-dependent increasing during the process of FSH-induced oocyte maturation. While the sense oligodeoxynucleotide (ODN) had no effect, antisense ODN inhibited FSH-induced LHR expression and meiotic resumption. Moreover, this antisense ODN against LHR could inhibit FSH-induced mitogen-activated protein kinase (MAPK) phosphorylation. This study suggested that LHR expression in cumulus cells is involved in FSH-induced oocyte meiotic resumption, which process is possibly regulated by MAPK cascade.
Keywords: Cumulus-enclosed Oocyte; LH Receptor; Oligodeoxynucleotide; Mouse
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