|
Passive Immunization Approach to Reduce Body Fat in Pigs Using Fat-specific Polyclonal Antiserum |
|
Kyung Hoon Baek, Chang Weon Choi, Chang Bon Choi, Byong Seak Lee |
|
|
|
|
|
|
|
| Abstract |
|
Plasma membrane proteins from pig adipocytes, brain, heart, kidney, liver and spleen were isolated using a 32% sucrose gradient. An adult male sheep was immunized three times at 3-wk intervals with the purified pig adipocyte plasma membrane (APM) proteins. Blood samples were taken from the immunized sheep 12 d after the third immunization. Antiserum showed strong reactivity with APM proteins determined by ELISA, and the reactivity could be detected at dilutions in excess of 1:128,000. Antiserum showed very low binding affinity with proteins isolated from brain, heart, kidney, liver or spleen. Ninety weanling pigs were allocated randomly to three treatment groups and were injected i.p. with 40 ml of antiserum (n = 30) or 20 ml of lyophilized antiserum (21.5 mg/ml; n = 30). A control group (n = 30) received 40 ml of saline, and all pigs were slaughtered at 24 wk of age. The polyclonal antiserum did not change BW or ADG. Carcass percentage of pigs was numerically increased by the antiserum treatment compared with control. Both antiserum treatments did not significantly (p>0.05) affect body composition, including body fat content, relative to the control group. |
| Keywords:
Pig; Adipocytes; Polyclonal Antiserum; ELISA; Carcass Percentage; Body Composition |
|
PDF Links
Full text via DOI
Download Citation
