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Swine Nutrition and Feed Technology
Asian-Australasian Journal of Animal Sciences 2007;20(3): 412-417.
DOI: https://doi.org/10.5713/ajas.2007.412    Published online January 24, 2007.
Nitrogen-15 Determination in Tissues of Laying Hens Fed on Different Levels of 15N-Chlorocholine Chloride (15N-CCC) Diets
Nurhayati*, Grete Thinggaard, S. Chakeredza, A. Reineking, R. Langel, U. ter Meulen
Correspondence:  Nurhayati,
Abstract
An experiment was conducted to determine the distribution of nitrogen-15 in tissues of laying hens receiving different levels of 15N-CCC in diets. Twenty brown laying hens were divided into four groups and randomly assigned into one of four dietary treatment groups consisting of 0, 5, 50 and 100 ppm 15N-CCC inclusion. The hens were individually fed with the 15N-CCC diets in battery cages for 11 days and then all hens restored to feeding on the control diet for 7 days. After eleven days, eight hens were slaughtered, and the others were slaughtered seven days after 15N-CCC diets withdrawal. Samples of blood, liver, heart and meat were collected and their 15N contents were determined. The δ15N excess (δ15N-ex) and atom percentage excess in 15N were calculated. The δ15N-ex and atom percentage excess 15N increased significantly (p<0.05) with increasing levels of 15N-CCC in diets in all tissues after feeding 15N-CCC diets for eleven days. The highest concentration of δ15N-ex and atom percentage excess 15N were detected in blood, followed in order by liver, heart and thigh meat. The concentrations reduced significantly (p<0.05) after 15N-CCC diets were withdrawn. Comparison between treatment groups showed that δ15N-ex and atom percentage excess 15N were still higher in hens that had been fed diets with higher levels of 15N-CCC. This study showed that nitrogen-15 was distributed in blood, liver, heart and meat of laying hens.
Keywords: Atom Percentage Excess Nitrogen-15; Delta Nitrogen-15 Excess; Laying Hen Tissues; Nitrogen-15
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